產(chǎn)品名稱(chēng)： 人精氨酸脫羧酶（ADC）ELISA Kit
 
 英文名稱(chēng)： Human Arginine Decarboxylase,ADC ELISA Kit
 
 貨號(hào)： CSB-E14914h
 
 規(guī)格： 96T
 
 種屬： Human
 
 待測(cè)物名稱(chēng)： arginine decarboxylase
 
 縮寫(xiě)： ADC
 
 蛋白功能1： Biosynthesis/Metabolism
 
 蛋白功能2： biogenic amine biosynthesis and metabolism
 
 蛋白功能3： Polyamine biosynthesis
 
 反應(yīng)時(shí)間： 1-5h
 
 所需樣本體積： 50-100ul
 
 檢測(cè)波長(zhǎng)： 450 nm
 
 用途： For research use only. Not for diagnostic use.
 
 原理 ： This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ADC has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ADC present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADC is added to the wells. After washing, avidin conjugated Horseradish Peroxidase （HRP） is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADC bound in the initial step. The color development is stopped and the intensity of the color is measured. 
 特異性： This assay has high sensitivity and excellent specificity for detection of Human ADC. No significant cross-reactivity or interference between Human ADC and analogues was observed. 
 精密度： Intra-assay Precision （Precision within an assay）: CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision （Precision between assays）: CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
 
 樣本搜集及儲(chǔ)存： Serum: Use a serum separator tube （SST） and allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 15 minutes at 1000 ×g. Remove serum and assay immediay or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles.
Plasma: Collect plasma using EDTA, or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 ×g at 2-8°C within 30 minutes of collection. Assay immediay or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles.
 
 檢測(cè)步驟： Bring all reagents and samples to room temperature before use. Centrifuge the sample again after thawing before the assay. It is recommended that all samples and standards be assayed in duplicate.
1. Prepare all reagents, working standards, and samples as directed in the previous sections.
2. Refer to the Assay Layout Sheet to determine the number of wells to be used and put any remaining wells and the desiccant back into the pouch and seal the ziploc, store unused wells at 4°C.
3. Add 100μl of standard and sample per well. Cover with the adhesive strip provided. Incubate for 2 hours at 37°C. A plate layout is provided to record standards and samples assayed.
4. Remove the liquid of each well, don't wash.
5. Add 100μl of Biotin-antibody （1x） to each well. Cover with a new adhesive strip. Incubate for 1 hour at 37°C. （Biotin-antibody （1x） may appear cloudy. Warm up to room temperature and mix gently until solution appears uniform.）
6. Aspirate each well and wash, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer （200μl） using a squirt bottle, multi-channel pipette, manifold dispenser, or autowasher, and let it stand for 2 minutes, complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining wash Buffer by aspirating ordecanting. Invert the plate and blot it against clean paper towels.
7. Add 100μl of HRP-avidin （1x） to each well. Cover the microtiter plate with a new adhesive strip. Incubate for 1 hour at 37°C.
8. Repeat the aspiration/wash process for five times as in step 6.
9. Add 90μl of TMB Substrate to each well. Incubate for 15-30 minutes at 37°C. Protect from light.
10. Add 50μl of Stop Solution to each well, gently tap the plate to ensure thorough mixing.
11. Determine the optical density of each well within 5 minutes, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. Subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.
 
 結(jié)果計(jì)算： Using the professional soft "Curve Expert 1.3" to make a standard curve is recommended, which can be downloaded from our web.
Average the duplicate readings for each standard and sample and subtract the average zero standard optical density.
Create a standard curve by reducing the data using computer software capable of generating a four parameter logistic （4-PL） curve-fit. As an alternative, construct a standard curve by plotting the mean absorbance for each standard on the x-axis against the concentration on the y-axis and draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the ADC concentrations versus the log of the O.D. and the best fit line can be determined by regression analysis. This procedure will produce an adequate but less precise fit of the data.
If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor.
 
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 Recombinant Escherichia coli Biosynthetic arginine decarboxylase（speA）  CSB-YP001330ENX  1mg  Yeast 
        
 Recombinant Shewanella woodyi Biosynthetic arginine decarboxylase（speA）  CSB-EP001330SYY  1mg  E.coli 
        
 Recombinant Shewanella woodyi Biosynthetic arginine decarboxylase（speA）  CSB-YP001330SYY  1mg  Yeast 
        
 Recombinant Shewanella haliensis Biosynthetic arginine decarboxylase（speA）  CSB-EP001330SYU  1mg  E.coli 
        
 Recombinant Shewanella haliensis Biosynthetic arginine decarboxylase（speA）  CSB-YP001330SYU  1mg  Yeast 
        
 Recombinant Xylella fastidiosa Biosynthetic arginine decarboxylase（speA）  CSB-EP001330XBM  1mg  E.coli 
        
 Recombinant Xylella fastidiosa Biosynthetic arginine decarboxylase（speA）  CSB-YP001330XBM  1mg  Yeast 
        
 Recombinant Arabidopsis thaliana Arginine decarboxylase 2（SPE2）  CSB-EP001330DOA  1mg  E.coli 
        
 Recombinant Arabidopsis thaliana Arginine decarboxylase 2（SPE2）  CSB-YP001330DOA  1mg  Yeast 
        
 Recombinant Geobacter sp. Biosynthetic arginine decarboxylase（speA）  CSB-EP001330GFR  1mg  E.coli 
        
 Recombinant Geobacter sp. Biosynthetic arginine decarboxylase（speA）  CSB-YP001330GFR  1mg  Yeast 
        
 Recombinant Tolumonas auensis Biosynthetic arginine decarboxylase（speA）  CSB-EP001330TOQ  1mg  E.coli 
        
 Recombinant Tolumonas auensis Biosynthetic arginine decarboxylase（speA）  CSB-YP001330TOQ  1mg  Yeast 
        
 Recombinant Hamiltonella defensa subsp. Acyrthosiphon pisum Biosynthetic arginine decarboxylase（speA）  CSB-EP001330HUG  1mg  E.coli 
        
 Recombinant Hamiltonella defensa subsp. Acyrthosiphon pisum Biosynthetic arginine decarboxylase（speA）  CSB-YP001330HUG  1mg  Yeast 
        
 Recombinant Geobacter sp. Biosynthetic arginine decarboxylase（speA）  CSB-EP001330GFP  1mg  E.coli 
        
 Recombinant Geobacter sp. Biosynthetic arginine decarboxylase（speA）  CSB-YP001330GFP  1mg  Yeast 
        
 Recombinant Shewanella baltica Biosynthetic arginine decarboxylase（speA）  CSB-EP001330SYS  1mg  E.coli 
        
 Recombinant Shewanella baltica Biosynthetic arginine decarboxylase（speA）  CSB-YP001330SYS  1mg  Yeast 
        
 Recombinant Shewanella piezotolerans Biosynthetic arginine decarboxylase（speA）  CSB-EP001330SYW  1mg  E.coli 
        
 Recombinant Shewanella piezotolerans Biosynthetic arginine decarboxylase（speA）  CSB-YP001330SYW  1mg  Yeast 
        
 Recombinant Escherichia coli O157:H7 Biosynthetic arginine decarboxylase（speA）  CSB-EP001330EOE  1mg  E.coli 
        
 Recombinant Escherichia coli O157:H7 Biosynthetic arginine decarboxylase（speA）  CSB-YP001330EOE  1mg  Yeast 
        
 Recombinant Salmonella schwarzengrund Biosynthetic arginine decarboxylase（speA）  CSB-EP001330SWV  1mg  E.coli 
        
 Recombinant Salmonella schwarzengrund Biosynthetic arginine decarboxylase（speA）  CSB-YP001330SWV  1mg  Yeast 
        
 Recombinant Salmonella agona Biosynthetic arginine decarboxylase（speA）  CSB-EP001330SWK  1mg  E.coli 
        
 Recombinant Salmonella agona Biosynthetic arginine decarboxylase（speA）  CSB-YP001330SWK  1mg  Yeast 
        
 Recombinant Klebsiella pneumoniae Biosynthetic arginine decarboxylase（speA）  CSB-EP001330KBH  1mg  E.coli 
        
 Recombinant Klebsiella pneumoniae Biosynthetic arginine decarboxylase（speA）  CSB-YP001330KBH  1mg  Yeast 
        
 Recombinant Salmonella dublin Biosynthetic arginine decarboxylase（speA）  CSB-EP001330SWN  1mg  E.coli 
        
 Recombinant Salmonella dublin Biosynthetic arginine decarboxylase（speA）  CSB-YP001330SWN  1mg  Yeast 
        
 Recombinant Salmonella newport Biosynthetic arginine decarboxylase（speA）  CSB-EP001330SWS  1mg  E.coli 
        
 Recombinant Salmonella newport Biosynthetic arginine decarboxylase（speA）  CSB-YP001330SWS  1mg  Yeast 
        
 Recombinant Geobacter bemidjiensis Biosynthetic arginine decarboxylase（speA）  CSB-EP001330GFN  1mg  E.coli 
        
 Recombinant Geobacter bemidjiensis Biosynthetic arginine decarboxylase（speA）  CSB-YP001330GFN  1mg  Yeast 
        
 Recombinant Salmonella heidelberg Biosynthetic arginine decarboxylase（speA）  CSB-EP001330SWQ  1mg  E.coli 
        
 Recombinant Salmonella heidelberg Biosynthetic arginine decarboxylase（speA）  CSB-YP001330SWQ  1mg  Yeast