Recombinant Human Dihydrofolate Reductase
人二氫葉酸還原酶 （DHFR）
Catalogue Number  ENZ-443

Synonyms:
Dihydrofolate reductase, DHFR, DHFRP1. 

Introduction:
Dihydrofolate reductase （DHFR） is an enzyme that reduces dihydrofolic acid to tetrahydrofolic acid, with NADPH as electron donor that can be converted to the kinds of tetrahydrofolate cofactors applied in 1-carbon transfer chemistry. DHFR converts dihydrofolate into tetrahydrofolate, which is a methyl group shuttle required for the de novo synthesis of purines, thymidylic acid, and specific amino acids. Even though the functional DHFR gene is mapped to chromosome 5, numerous intronless processed pseudogenes or dihydrofolate reductase-like genes are identified on separate chromosomes.
DHFR deficiency is associated with megaloblastic anemia.
DHFR knockdown plays a role in the anticancer activity of 2-hydroxyoleic acid.
DHFR gene insertion/deletion polymorphism is linked to variation in serum and red blood cell folate concentrations in women. 

Description:
DHFR Human Recombinant fused with 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 207 amino acids （1-187 a.a.） and having a molecular mass of 23.6kDa.
The DHFR is purified by proprietary chromatographic techniques. 

Source:
Escherichia Coli. 

Physical Appearance:
Sterile Filtered colorless solution. 

Formulation:
The DHFR solution contains 20mM Tris-HCl buffer （pH8.0）, 0.1M Nacl 2mM DTT, and 30% glycerol. 

Stability:
DHFR although stable 4°C for 4 weeks, should be stored desiccated below -18°C. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please prevent freeze-thaw cycles. 

Purity:
Greater than 95.0% as determined by SDS-PAGE. 

Biological activity:
MGSSHHHHHH SSGLVPRGSH MVGSLNCIVA VSQNMGIGKN GDLPWPPLRN EFRYFQRMTT TSSVEGKQNL VIMGKKTWFS IPEKNRPLKG RINLVLSREL KEPPQGAHFL SRSLDDALKL TEQPELANKV DMVWIVGGSS VYKEAMNHPG HLKLFVTRIM QDFESDTFFP EIDLEKYKLL PEYPGVLSDV QEEKGIKYKF EVYEKND. 

Usage:
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. 
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