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化工儀器網(wǎng)>產(chǎn)品展廳>試劑標物>行業(yè)專用試劑>生物試劑> CRL-2580MR1中國倉鼠X小鼠B淋巴細胞雜交瘤

CRL-2580MR1中國倉鼠X小鼠B淋巴細胞雜交瘤

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  • 公司名稱 上海復祥生物科技有限公司
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ATCC 細胞

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上海復祥生物科技有限公司是一家專業(yè)從事原代細胞、細胞系,；菌種,；及細胞因子相關(guān)產(chǎn)品的銷售企業(yè)。公司建有自己的細胞庫和菌種庫,。細胞庫管理規(guī)范,，提供的細胞株背景清楚，提供參考文獻和*培養(yǎng)條件,，公司引進ATCC細胞500余種,，ATCC菌種800余種。并和國內(nèi)各大保藏中心有密切聯(lián)系,，細胞種類齊全,，質(zhì)量保證！是生命科學領(lǐng)域*的一部分,！

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ATCC 細胞,細胞系,細胞株,腫瘤細胞,細胞,ATCC 菌種,，CMCC 菌種，標準菌株,，質(zhì)控菌種,，微生物菌種，菌株,，菌種

供貨周期 一周 規(guī)格 T25
應用領(lǐng)域 醫(yī)療衛(wèi)生,化工,生物產(chǎn)業(yè),制藥 主要用途 科學研究

CRL-2580 MR1 中國倉鼠X小鼠B淋巴細胞雜交瘤,，ATCC 細胞|細胞系|細胞株|腫瘤細胞|細胞|貼壁細胞|懸浮細胞|,細胞庫管理規(guī)范,提供的細胞株背景清楚,提供參考文獻優(yōu)培養(yǎng)條件

CRL-2580 MR1 中國倉鼠X小鼠B淋巴細胞雜交瘤 的詳細介紹


ATCC® Number:CRL-2580™  Price:$323.00
Designations:MR1 [derivative of HB-11048]

Depositors:Bristol-Myers Squibb Pharm. Res. Inst.

Isotype:IgG

Biosafety Level:1

Shipped:frozen

Medium & Serum:See Propagation





Organism:Cricetulus migratorius （B cell）; Mus musculus （myeloma） （hamster, Armenian （B cell）; mouse （myeloma））

Morphology:lymphoblast


Source:Cell Type: hybridoma: B lymphocyte;

Cellular Products:immunoglobulin; monoclonal antibody; against mouse CD40 ligand （CD154, CD40L, gp39）

Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.



Comments:Animals were immunized with mouse T cells （Th1）.

Spleen cells were fused with NS-1 myeloma cells.

The antibody detects a 39000 dalton protein selectively expressed on activated mouse T cells.

A culture deposited at the ATCC as HB-11048 in May of 1992 was found to be contaminated with mycoplasma. Progeny were cured by a 21-day treatment with BM Cycline.



Propagation:ATCC complete growth medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate and supplemented with 0.05 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%
Temperature: 37.0°C

Subculturing:Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 to 3 X 10 exp5 viable cells/ml. Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml.

Preservation:Freeze medium: Complete growth medium, 92.5%; DMSO, 7.5%
Storage temperature: liquid nitrogen vapor phase

Related Products:Recommended medium （without the additional supplements or serum described under ATCC Medium）:ATCC 30-2001

recommended serum:ATCC 30-2020

parental cell line:ATCC HB-11048



References:23334: Noelle RJ, et al. A 39-kDa protein on activated helper T cells binds CD40 and transduces the signal for cognate activation of B cells. Proc. Natl. Acad. Sci. USA 89: 6550-6554, 1992. PubMed: 1378631

23483: Roy M, et al. The regulation of the expression of gp39, the CD40 ligand, on normal and cloned CD4+ T cells. J. Immunol. 151: 2497-2510, 1993. PubMed: 8103067


CRL-2580 MR1 中國倉鼠X小鼠B淋巴細胞雜交瘤


















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