Background: The human CD3/T cell receptor （TcR） complex is made up of at least five CD3 proteins （gamma, delta, epsilon, h, z） in association with either alpha/beta or gamma/delta proteins of the TcR （2）. The TcR recognizes antigens in association with MHC molecules after which protein chains of the CD3 complex mediate activation signals triggered by TcR antigen binding. CD3 is expressed on greater than 95% of circulating human peripheral T cells. The CD3 complex mediates signal transduction. Subcellular location: Membrane; Single-pass type I membrane protein.
Specificity : · anti- CD3 is a rabbit polyclonal antibody unconjugated
· specific for CD3 of human, mouse, rat, rabbit, pig and cattle original
· epitope mapping in the middle of CD3 of human, mouse, rat, rabbit
and pig, original. recognizes the 20 kd epsilon chain （3） of the CD3
molecule complex
· Protein G affinity chromatography purification, purity :>95%
· Isotype: IgG
· use for western blotting, elisa, immunoprecipitation and
Immunohistochemistry
· mol wt: Recognizes 20 kDa
· Optimal working dilutions must be determined by the end user.
Storage: Store at –20 ºC for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20ºC. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody, the antibody is stable for at least two weeks at 2-4 ºC.
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
提供商 |
北京索萊寶科技有限公司 |
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