EvoScript RNA 探針大師

EvoScript RNA 探針大師

EvoScript RNA Probes Master 專為靈敏,、高特異性、高精度的一步法 RT-qPCR 而設(shè)計(jì),，尤其適用于基因表達(dá)分析。

For general laboratory use.

供一般實(shí)驗(yàn)室使用,。

Pack Size：200 reactions  包裝尺寸：200 反應(yīng)

Overview概述：

The EvoScript RNA Probes Master as a 1-vial composition, is designed for sensitive, high-specificity, high-precision one-step RT-qPCR especially for gene expression assays.

EvoScript RNA Probes Master 為 1 瓶組合物,，專為靈敏、高特異性,、高精度的一步法 RT-qPCR 而設(shè)計(jì),，尤其適用于基因表達(dá)分析。

Benefits  好處

Ease of use, convenience, speed, reduced error

易于使用,、方便,、快速、減少錯(cuò)誤

Result consistency, stability and tolerance of targets of varying secondary structure

不同二級(jí)結(jié)構(gòu)目標(biāo)的結(jié)果一致性,、穩(wěn)定性和耐受性

Ability to detect low copy or rare transcripts for gene expression applications

能夠?yàn)榛虮磉_(dá)應(yīng)用檢測(cè)低拷貝或稀有轉(zhuǎn)錄本

Superior quantification capability for gene expression applications

基因表達(dá)應(yīng)用的定量能力

Easier assay optimization

更輕松的分析優(yōu)化

Contamination control capability

污染控制能力

Generate consistent and robust results, no matter what sample type you are starting from or what target you are detecting

生成一致且可靠的結(jié)果,，無論您從什么樣本類型開始或您正在檢測(cè)什么目標(biāo)

Rely on a higher sensitivity when detecting low copy numbers, with the input of larger sample volumes into the reaction

在檢測(cè)低拷貝數(shù)時(shí)依靠更高的靈敏度，將更大的樣品體積輸入到反應(yīng)中,、

Obtain fast and specific results

獲得快速和具體的結(jié)果

Contents內(nèi)容

1. EvoScript RNA Probes Master, 5× conc.

    EvoScript RNA Probes Master,，5倍濃度。

2. Water, PCR Grade  

 水,，PCR 級(jí)

Application應(yīng)用：

The EvoScript RNA Probes Master is designed for sensitive, high-specificity, high-precision one-step RT-qPCR especially for gene expression assays. The 1-vial composition is ideally suited for easy reaction assembly, requiring only the addition of oligos, primers and target RNA. The proprietary reaction buffer allows a convenient hot start RT-qPCR. In addition, the enzymes for reverse transcription and for PCR feature hot start for premium specificity1). The master mix is optimized for hydrolysis probes, including Universal Probe Library (UPL) probes and does not require optimization with Mg(OAc)2.

1) The reverse transcription activity will be suppressed at temperatures significantly below 60°C. Primer

EvoScript RNA Probes Master 專為靈敏,、高特異性、高精度的一步法 RT-qPCR 而設(shè)計(jì),，尤其適用于基因表達(dá)分析,。1-vial 組合物非常適合簡(jiǎn)單的反應(yīng)組裝，只需要添加寡核苷酸,、引物和目標(biāo) RNA,。專有的反應(yīng)緩沖液允許方便的熱啟動(dòng) RT-qPCR。此外,，用于逆轉(zhuǎn)錄和 PCR 的酶具有熱啟動(dòng)特性,，具有的特異性1)。預(yù)混液針對(duì)水解探針進(jìn)行了優(yōu)化,，包括通用探針庫 (UPL) 探針,，不需要使用 Mg(OAc) 2進(jìn)行優(yōu)化。

1) 顯著低于 60°C 的溫度會(huì)抑制逆轉(zhuǎn)錄活性,。底漆