羅氏雙雜交探針®尿嘧啶-DNA 糖基化酶 

羅氏雙雜交探針®尿嘧啶-DNA 糖基化酶 

LightCycler® Uracil-DNA Glycosylase  

LightCycler ® 尿嘧啶-DNA糖基化酶水解單鏈和雙鏈DNA中U-DNA位點的尿嘧啶-糖苷鍵,，切除尿嘧啶并在DNA中產(chǎn)生對堿和熱敏感的脫堿基位點,。

該酶對單鏈 DNA 的活性高于對雙鏈 DNA 的活性

該酶對 RNA 和天然無尿嘧啶 DNA 無活性。

Contents 內(nèi)容

The enzyme is supplied in storage buffer at 2 U/µl.

該酶以 2 U/µl 的濃度提供在儲存緩沖液中,。

Application應(yīng)用：

LightCycler® Uracil-DNA Glycosylase (LightCycler® UNG) is ideally suited for preventing carryover contamination between PCRs. This technique involves incorporating deoxyuridine triphosphate (dUTP) into all amplification reactions, and pretreating all successive LightCycler® FastStart Enzyme-based PCR mixtures with LightCycler® UNG. Prior to the thermal-cycling procedure, LightCycler® UNG is added to the LightCycler® amplification reaction mixture, then incubated for 10 minutes at +40°C. During the initial activation of FastStart DNA Polymerase (+95°C for 10 minutes), the LightCycler® UNG becomes inactive.

LightCycler ® 尿嘧啶-DNA 糖基化酶 (LightCycler ®  UNG) 非常適合防止 PCR 之間的殘留污染,。該技術(shù)包括將脫氧尿苷三磷酸 (dUTP) 加入所有擴增反應(yīng)中，并使用 LightCycler ®  UNG預(yù)處理所有連續(xù)的基于LightCycler ®  FastStart 酶的 PCR 混合物,。在熱循環(huán)程序之前,，將 LightCycler ®  UNG 添加到 LightCycler ® 擴增反應(yīng)混合物中，然后在 +40°C 下孵育 10 分鐘,。在 FastStart DNA 聚合酶的初始激活期間（+95°C 10 分鐘）,，LightCycler ®  UNG 變得不活躍。

Note: LightCycler® UNG is designed only for use with the FastStart Enzyme or FastStart Enzyme-based LightCycler® Kits.

注意： LightCycler®  UNG 僅設(shè)計用于 FastStart 酶或基于 FastStart 酶的 LightCycler ® 試劑盒,。