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BD Horizon™ Fixable Viability Stain 700 564997

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江蘇省科學(xué)器材有限公司成立于1963年,總部位于江蘇省南京市,,是一個有著超60經(jīng)營實(shí)驗(yàn)室設(shè)備/耗材及分析儀器的綜合供應(yīng)商,,公司具有自營和代理各類商品和技術(shù)的進(jìn)出口權(quán)。

 

2005年公司根據(jù)省政府有關(guān)文件,,在省科技廳的直接領(lǐng)導(dǎo)下,,整體改制成為股份制公司。

 

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BD Horizon™ Fixable Viability Stain 700 564997


BD Horizon™ Fixable Viability Stain 700 564997

BD Horizon™ Fixable Viability Stain 700 (FVS700) is useful for discrimination of viable from non-viable mammalian cells in multicolor flow cytometric applications. This dye reacts with and covalently binds to cell-surface and intracellular amines. Permeable plasma cell membranes, such as those present in necrotic cells, allow for the intracellular diffusion and covalent binding of the dye to the higher levels of total cellular amines when compared to living cells with impermeable plasma membranes.  Therefore, necrotic cells present in a typical in vitro assay label with higher levels of dye increasing their FVS700 fluorescence intensities 10-20 fold over that of viable cells. The FVS700-labeled cells can be fixed with formaldehyde for downstream decontamination, freezing and/or permeabilization and subsequent intracellular staining while maintaining stable viability stain fluorescence.


BD Horizon™ Fixable Viability Stain 700 is excited by the Red laser (with an excitation maximum of 657 nm), and has a fluorescence emission maximum of 700 nm.



BD Horizon™ Fixable Viability Stain 700 564997


Flow cytometric analysis of human Jurkat cells stained with BD Horizon™ Fixable Viability Stain 700. Cells from the human Jurkat (Acute T cell leukemia, ATCC TIB-152) cell line were treated with 0.025% DMSO (Left Panel) or 5 μM camptothecin (Right Panel) for 16 hours and then stained with BD Horizon™ Fixable Viability Stain 700 (Cat. No. 564997) in serum-free buffer. The cells were then either left unfixed (solid line histograms) or fixed in BD Cytofix™ Fixation Buffer (Cat. No. 554655) and permeabilized in BD Phosflow™ Perm/Wash Buffer I (Cat. No. 557885, dashed line histograms). Histograms were derived from gated events with the forward and side light-scattering characteristics of Jurkat cells. Flow cytometric analysis was performed using a BD LSRFortessa™ Flow Cytometry System. Please note that FVS700 is also compatible with BD Phosflow™ Perm Buffer III (Cat. No.558050) or BD Pharmingen™ Transcription Factor Buffer Set (Cat. No. 562574/562725). Fixable Viability Stain 700 has been tested on mouse (data not shown).




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