cellsignal 細胞檢測試劑盒  BrdU Cell Proliferation Assay Kit 貨號：6813S

Figure 1.C2C12 cells wereseeded at varying density inserum free medium in a 96-well plate and incubatedovernight. Serum was addedto the plate at variousconcentrations and cells wereincubated for 24 hr Finally,10μM BrdU was added to theplate and cells were incubatedfor 4 hr.

Figure 2.Treatment of MCF10A cells with HumanEpidermal Growth Factor(hEGF) #8916 increases cellproliferation as detected byBrdU Cell Proliferation AssayKit #6813. MCF 10A cells wereseeded at 1x104 cells/well in a96-well plate and incubatedovernight. Cells were thenstarved in serum free mediumovernight. hEGF was added tothe plate and cells wereincubated for 24 hr. Finally, 10μM BrdU was added to theplate and cells were incubatedfor 4 hr.

Figure 3. jurkat cells wereseeded at 4x104 cells/well in a96-well plate and incubatedovernight. Cells were thentreated with variousconcentrations of doxorubicinfor 2 hr. Finally, 10 μM BrdUwas added to the plate andcells were incubated for 4 hr.

BrdU 細胞增殖檢測試劑盒使用抗 BrdU 抗體檢測細胞增殖過程中摻入細胞 DNA 的 5-溴-2'-脫氧尿嘧啶 （BrdU）,。當(dāng)使用含有 BrdU 的標記培養(yǎng)基培養(yǎng)細胞時，這種嘧啶類似物代替胸苷摻入增殖細胞新合成的 DNA 中,。去除標記培養(yǎng)基后,，固定細胞，并使用我們的固定/變性溶液使 DNA 變性,。然后加入 BrdU 小鼠單克隆抗體以檢測摻入的 BrdU（DNA 變性對于提高摻入的 BrdU 對檢測抗體的可及性是必要的）,。然后使用抗小鼠 IgG、HRP 連接的抗體來識別結(jié)合的檢測抗體,。添加 HRP 底物 TMB 以顯色,。顯色的吸光度大小與摻入細胞中的 BrdU 的數(shù)量成正比，這是細胞增殖的直接指標,。

特異性/靈敏度

該產(chǎn)品僅用于研究,，不能用于人體,。

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cellsignal 細胞檢測試劑盒  BrdU Cell Proliferation Assay Kit 貨號：6813S