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上海乾思生物科技有限公司
中級會員 | 第17年

18916423352

細胞庫
優(yōu)勢品牌
Encapsula NanoSciences Quidel Bioporto Salimetrics Research Products International Corp Quanta BioDesign Peninsula Laboratories International Golden West Diagnostics Ocean NanoTech HaemoScan affinitybiologicals Cytodiagnostics Innovagen AB CLIAwaived Svar Abbexa ABMgood AffinityImmuno AGScientific AHF analysentechnik AG Alomone 英國Antibodies AstaTech Avidity Badrilla BioAcademia Biomatik Biosynth BioTeZ Biotrend Calbiotech CaroteNature Cell Sciences Click Chemistry Tools Cohesion Biosciences CordenPharma Cosmo Bio Creative Biomart Crystal Chem Crystalgen Diagnovum DiaMetra Dianova Diatheva Eagle Manufacturing Echelon Biosciences Edgebio Emfret Epoch Life Science Equitech-Bio Eramask Euro Diagnostica Exocell Fine Science Tools GenwayBio Giotto Biotech Glen Research Glycosynth Gold Biotechnology Golden West Biologicals Haemtech(HTI) Herolab IHC WORLD ImmuChrom GmbH Immunostep Immunotools Immunovision INCELL Corporation Innovative Research Innovrsrch(IRA) Insitus Iris Biotech GmbH Isosep IST Engineering Inc J.G. Finneran Kainos Kamiya Biomedical Labor Diagnostika Nord Larodan Laysanbio Lc laboratories Lee BioSolutions LGC Biosearch Technologies Lifecore List Labs lumiprobe Macrocyclics Medix Biochemica Meridian Life Science Microbix Milenia Biotec Moscerdam Mybiosource Nanoimmunotech Nanoprobes Neuro Probe Paratechs Physitemp Polypure Polysciences Probetex Progen ProScience Inc ProSpec QAbio Qorpak Quanta BioSciences QuickZyme RevMAb Biosciences SCHMIDT BioMedTech Seven Hills Foundation Smartox Biotechnology Spherotech Surgimax Swant SYSY Ted Pella Torpac Trilink USBio VIDIA Ltd ViroStat Virusys VP-scientific Xcessbio XENOMETRIX Zeptometrix ZYTOVISION
自產(chǎn)ELISA試劑盒,試劑盒
進口ELISA試劑盒
人細胞,人細胞系,人細胞株
3h biomedical,3H Biomedical AB,3hbiomedical,3-M Company,4adi,4rtilab,5 prime,5prime a & g pharmaceuticals,A&D Weighing,Aalto Scientific,Aalto Scientific ltd,Aalto Scientific,ltd.,aaltobioreagents,aaltoscientific,aat bioquest AAT Bioquest Inc,AB Biolabs,ab peptides,AB Sciex,Abaxis,Abazyme,Abberior,abbexa abbiotec,Abbkine,abbomax,Abbott Molecular,abcam,ABclonal,ABclonal Inc.,abcore-inc abcr GmbH,abcrGmbH,abcysonline,ABD,ABD Bioquest,Inc,abd serotec,ab-direct,abdserotec Abeomics,abfrontier,abgab,abgene,abgent,ABI Applied Biosystems,abiocode,abionline Abkem Iberia,ABL,Inc,Abliance,abm Inc.,abmart,abmgood,Abmole,Abmole Bioscience Inc. abnova,Abraxis,Absolute Antibody,ABT Beads,abt beads technologies,ABTbeads,abvision,Abways abzoom,academy biomed,academy bio-medical,acanthus research,acanthusresearch,ACC,accelagen,Accelagen inc. Access Biologicals,LLC,accessbiologicals,acciusa,accu-chek,AccuDiag Allergy,AccuDiag Anemia,AccuDiag Autoimmune Diseases,AccuDiag Bone Metabolism AccuDiag Thyroid,accumax accutase,Accumetrics,Accurate,accurate chemical,Accurate Wirecraft Company,accuratechemical,accustandard acdbio,Ace Glass,Ace Hose and Rubber Co,ACEA Biosciences,ACL Incorporated,ACME Microspheres Inc,ACMEmicrospheres,acris acris antibodies,Acris Antibodies GmbH,acris-antibodies,ACROBiosystems,acrometrix,acros,Acros Organics,ACT Test Panels LLC actc,actgene,active motif,activemotif,Adam Equipment,Inc,AddexBio,Addexbio Technologies,addgene ademtech,adg,ADI,ADInstruments,adipogen,Adolf Kuhner,adooq,Advance Tabco advanced immunochemical,advanced immunochemical inc,advanced targeting systems,Advansta,Advansta Inc.,Advansta,Inc,Advantech,Advent Electric Inc adventbio,advnt,advtechind,advy chemical,advy chemical Purified Proteins,advychemical Polyclonal Antisera,aesku,aetltd affiland,affinity,affinity biologicals,affinity bioreagent,Affinity BioReagents,Affinity BioReagents abr,Affinity Biosciences,affinitybiologicals AffinityBioReagents,AffinityImmuno,affix scientific,affixscientific,affymetrix,ag scientific,Agar Scientific,agarscientific agdia,Agela Technologies,agilent,Agilent Seahorse,agilent technologies,agilet,agilet solutions,agiletsolutions agrenvec,agrisera,AGRO-BIO,agscientific,ahdiagnostics,ahf,AHF analysentechnik,AHF analysentechnik AG Air Technology Solutions,Airgas Safety,Air-Tite,AJAX SCIENTIFIC LTD,AK Limited,Akro-Mils,ALA Scientfic,alamarblue Alba Bioscience,Alconox Inc,aldevron,alerchek,alere,Alexis,ALEXIS Biochemicals,Alfa Aesar Aline Biosciences,AlineBiosciences,All Foils Incorporated,Allcells,allele biotech,allelebiotech,allen-bradley,AllerMAbs
大鼠細胞,大鼠細胞系
細胞株,細胞系,傳代細胞
抗體庫,小包裝,流式抗體
SIGMA現(xiàn)貨,SIGMA代理

抗體定制技術(shù)服務|實驗技術(shù)服務

時間:2015/7/1閱讀:290
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關(guān)鍵詞:抗體定制技術(shù)服務|實驗技術(shù)服務
簡介:世界*品牌抗體定制技術(shù)服務|實驗技術(shù)服務原裝,質(zhì)量保證,*,。
抗體定制技術(shù)服務|實驗技術(shù)服務——pCzn1質(zhì)粒+Arctic Express宿主菌低溫表達體系。
我們具備豐富的蛋白表達設(shè)計經(jīng)驗及實驗操作技巧,,承諾客戶不成功不收費,。我們所有的實驗數(shù)據(jù)真實可信,我們提供原始的表達菌株和克隆質(zhì)粒,,客戶可以依據(jù)我們的實驗報告重復我們的實驗結(jié)果。
產(chǎn)品品牌:   http://www.,。,。com/goodsid/fenleiyi/2868603/1.html    
測序?qū)嶒灹鞒蹋?br>抗體檢測服務
ELISA(酶聯(lián)免疫吸附實驗)
Dot Blot(斑點印跡實驗)
Western Blot(免疫印跡實驗)
IHC (免疫組織化學實驗)
抗體標記服務
HRP(辣根過氧化物酶)
Biotin(生物素)
操作方法
⑴準備好脾細胞,。
⑵在50ml沉淀管中混合108脾細胞和107小鼠骨髓瘤細胞,并加入50ml2.5%FCS-1640液,。
⑶室溫400g離心3min,,使細胞沉淀。
⑷移去上清液,。
⑸輕敲管底部,,使沉淀流動,把沉淀管放于40℃水浴中,,使其達融合溫度,。
⑹加預熱至40℃的50%PEG0.8ml,,用1ml吸管緩慢滴加,,邊加邊搖沉淀管,,肉眼觀察可見有顆粒出現(xiàn),滴加過程要求持續(xù)2min,。
⑺加1ml1640液,,邊加邊搖動,持續(xù)1min,。
⑻重復⑺一次,。
⑼加1ml1640液,邊加邊搖動,,持續(xù)0.5min,。
⑽重復⑼一次。
⑾加1640液15ml,。
⑿室溫,,400g離心1min,使細胞沉淀,。
⒀去上清,,輕敲管底部,加25ml含有HAT的*1640液,。
⒁往已于前一日種有飼養(yǎng)細胞的40孔塑料培養(yǎng)盤中滴加融合的細胞液,,每孔1滴(約0.05ml)。
⒂輕搖后,,放入5%CO2飽和濕度的37℃溫箱中培育,。
⒃第3、6,、9,、10日換入含HAT的*1640培養(yǎng)液。注意輕輕吸取上清液,,勿將固定于孔底的細胞吸出,,根據(jù)需要加入適量的飼養(yǎng)細胞。
⒄于第12,、15日加入含有HAT的*1640培養(yǎng)液,。在每次換液前用倒置顯微鏡觀察,大約在10天左右就可觀察到雜交瘤細胞生長出來,。大多數(shù)雜交瘤細胞在10~20天內(nèi)出現(xiàn),,但也有在1個月左右才能出現(xiàn)的。雜交瘤細胞出現(xiàn)后,,吸取上清液,,檢查抗體。
⒅對繼續(xù)生長的雜交瘤細胞進行增殖傳代。在傳代過程中,,依情況取消HAT液和*1640液而代之以10%FCS-1640液,。同時保存于液氮和進行克隆化,在這期間每代都要檢查抗體,,以防止產(chǎn)生抗體細胞的變異和丟失,。

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