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The?K-ε-GV?motif?refers?to?the?glycine?and?valine?remnant?left?on?the?lysine?residue?of?a?protein?after?Ufmylation,?a?form?of?post-translational?modification?（PTM）.?In?proteomics?research,?the?analysis?of?the?K-ε-GV?motif?typically?involves?the?use?of?specific?antibodies?to?enrich?these?modified?peptides,?followed?by?identification?and?quantification?using?mass?spectrometry?techniques.?This?analytical?approach?helps?researchers?better?understand?the?role?of?ufmylation?in?biological?processes?and?how?it?changes?under?disease?conditions.?Furthermore,?antibodies?against?the?K-ε-GV?motif?are?also?used?in?Ufmylation?proteomics?technology,?which?combines?immunoaffinity?enrichment?with?liquid?chromatography-tandem?mass?spectrometry?（LC-MS/MS）?for?large-scale?qualitative?and?quantitative?analysis?of?Ufmylatinated??proteins.