RNAi基因敲除的優(yōu)點(diǎn)及應(yīng)用

①.干細(xì)胞比用同源重組法更加簡(jiǎn)便,，周期大大縮短,。

②.對(duì)于哺乳動(dòng)物,，如對(duì)于一些敲除后小鼠在胚胎時(shí)就會(huì)死亡的基因,，可以在體外培養(yǎng)的細(xì)胞中利用RNAi技術(shù)研究它的功能,。

③.由于RNAi能特異的阻斷基因的表達(dá),，它成為研究信號(hào)傳導(dǎo)通路的良好工具。

④.RNAi還被用來(lái)研究在發(fā)育過(guò)程中起作用的基因,，如可用RNAi來(lái)阻斷某些基因的表達(dá),，來(lái)研究他們是否在胚胎干細(xì)胞的增殖和分化過(guò)程中其起著關(guān)鍵作用。

YAL011W BY4743, homozygous diploid [30397]    Saccharomyces cerevisiae Meyen ex E.C. Hans ..
YLR262C-A BY4743, heterozygous diploid [2602 ..    Saccharomyces cerevisiae Meyen ex E.C. Hans ..
W    Hypocrea parapilulifera
YML059C BY4743, heterozygous diploid [20511]    Saccharomyces cerevisiae Meyen ex E.C. Hans ..
yD2890    Homo sapiens, human
W-10, 75, 78, 126(-) 1150 2-5    Ascobolus immersus Persoon
S5    Burkholderia sp.
yD6457 [I66G3]    Homo sapiens, human
YIL103W BY4743, heterozygous diploid [22262]    Saccharomyces cerevisiae Meyen ex E.C. Hans ..
M [CIP 103003, DSM 3133]    Actinotalea fermentans (Bagnara et al.) Yi ..
T/G HA-VSMC    Homo sapiens, human aorta/smooth muscle
EU-47 P45-4    Cryphonectria parasitica (Murrill) Barr, te ..
YBR022W BY4743, homozygous diploid [33159]    Saccharomyces cerevisiae Meyen ex E.C. Hans ..
YHR184W BY4743, homozygous diploid [32878]    Saccharomyces cerevisiae Meyen ex E.C. Hans ..
HD 9468    Haemophilus ducreyi (Neveu-Lamaire) Bergey ..
205    干細(xì)胞Marinobacterium jannaschii (Bowditch et al ..