TREM2 Effector Reporter Cell
| I. Background | |
TREM2是免疫球蛋白超家族的單次跨膜受體,，主要表達(dá)于巨噬細(xì)胞、未成熟單核細(xì)胞 衍生的樹突狀細(xì)胞,、破骨細(xì)胞和小膠質(zhì)細(xì)胞上,。TREM2 包含免疫球蛋白 V 型結(jié)構(gòu)的胞外 域、跨膜域和沒(méi)有信號(hào)傳導(dǎo)基序的短胞內(nèi)結(jié)構(gòu)域,。它通過(guò) DNAX 激活蛋白 10 和 12 （DAP10 和 DAP12）發(fā)出信號(hào),。單個(gè) TREM2 分子可以同時(shí)與 DAP10 和 DAP12 相互作 用。 | |
| II. Description | |
TREM2 Effector Reporter 報(bào)告基因藥靶模型很好的模擬了體內(nèi) TREM2 的信號(hào)轉(zhuǎn)導(dǎo)過(guò)程， 原理見下圖所示,。
Figure 1. TREM2 Effector Reporter 細(xì)胞模型原理圖 | |
| III. Introduction | |
| Expressed gene: | TREM2 |
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | RPMI-1640+10%FBS+1 μg/ml Puromycin+10 μg/ml Blasticidin+800 μg/ml Hygromycin B |
| Mycoplasma Testing: | Negative |
| Storage: | Liquid nitrogen |
| Application(s): | Functional(Report Gene) Assay |
| IV. Representative Data | |
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Figure 3. Dose Response of TREM2 Agonist Ab in TREM2 Effector Reporter Cells (C14). | |
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