壓電細(xì)胞微管吸吮系統(tǒng)

計(jì)算機(jī)控制的全自動(dòng)微吸管系統(tǒng)

該系統(tǒng)是一種安裝在正常倒置顯微鏡上的計(jì)算機(jī)控制的微量吸管系統(tǒng),，

特點(diǎn)：

• 1)High throughput single cell sorting directly from the Petri dish

• One single cell arrives to each PCR tube

• 10 PCR strips containing 80 tubes can be filled in a cycle

• Glass cover slip for testing single cell deposition in situ

• Drop volume less than 1 ul for adherent cells

• Pick up volume of ~1 nl for suspended cells

• 15-20 seconds per cell. When collecting multiple cells, sorting speed is 1 cell/second.

• Number of cells picked up in a single run: 1-1000.

• Isolates a subpopulation of live adherent cells expressing fluorescent or luminescent markers

• Both unlabeled and fluorescentcells are recognized by computer vision

• Viable cells after sorting

• Any adherent and non-adherent cell type can be sorted

• Cell culture needs minimal preparation before sorting

• Average sorting process takes only a few minutes

• Multichannel detection using the fluorescent filter setup of the microscope

應(yīng)用范圍：

1）計(jì)算機(jī)控制的自動(dòng)化微吸管系統(tǒng)測定單細(xì)胞黏附力（Single Cell Adhesion Assay Using Computer Controlled Micropipette）

2）計(jì)算機(jī)控制的自動(dòng)化微吸管系統(tǒng)測定細(xì)胞與細(xì)胞之間的黏附力（Cell-cell adhesion force assay Using Computer Controlled Micropipette）

1. R. Salánki et al. : Single cell adhesion assay using computer controlled micropipette, PLoS ONE 9(10): e111450 (2014) Open access paper.

2. P. K. Jani et al.: Complement MASP-1 enhances adhesion between endothelial cells and neutrophils by up-regulating E-selectin expression, Molecular Immunology 75, 38–47 (2016)

3. N. Sándor et al.: CD11c/CD18 Dominates Adhesion of Human Monocytes, Macrophages and Dendritic Cells over CD11b/CD18, PLoS ONE 11(9), e0163120 (2016) Open access paper.

其他應(yīng)用：

3）單細(xì)胞分離與抓取分選系統(tǒng) （Single Cell Isolation and Sorting）

1. Z. K?rnyei et al.: Cell sorting in a Petri dish controlled by computer vision Nature Scientific Reports 3, Article number: 1088 (2013) Open access paper.

2. R. Salánki et al.: Automated single cell sorting and deposition in submicroliter drops, Appl. Phys. Lett. 105, 083703 (2014)

3. R. Salánki et al.: High-throughput image based single cell isolation, Microscopy and Analysis, January issue, S10-13 (2015) Open access paper.

4. R. Ungai-Salánki et al.: Automated single cell isolation from suspension with computer vision, Scientific Reports 6, Article number: 20375 (2016) Open access paper.

4）循環(huán)腫瘤細(xì)胞（CTC）分離 （Circulating tumor cell (CTC) isolation）

Marnie Winter et al.: Isolation of Circulating Fetal Trophoblasts Using Inertial Microfluidics for Noninvasive Prenatal Testing, Advanced Materials Technologies 1800066 (2018)

5）單細(xì)胞RNA測序 （Single Cell RNA Sequencing）應(yīng)用文獻(xiàn)

1. Mia Palmkvist: Malaria and polypeptides of plasmodium falciparum at the infected erythrocyte surface, PhD Thesis, Karolinska Institutet, Stockholm (2016)

2. A. Kozlov et al.: A screening of UNF targets identifies Rnb, a novel regulator of Drosophila circadian rhythms, The Journal of Neuroscience  7, 3286-16 (2017)

3. M. Ngara et al.: Exploring parasite heterogeneity using single-cell RNA-seq reveals a gene signature among sexual stage Plasmodium falciparum parasites, Experimental Cell Research (2018)

6）蛋白工程（Potein Engineering)

1. K. Piatkevich et al. : A robotic multidimensional directed evolution approach applied to fluorescent voltage reporters, Nature Chem Biol, doi:10.1038/s41589-018-0004-9 (2018)

2.