上海恒遠生物科技有限公司專業(yè)代理美國進口原裝,、分裝ELISA試劑盒，種屬標(biāo)本齊全,，待檢樣本可以是體液,，血清,，血漿,，細胞培養(yǎng)上清液,，尿液,，組織勻漿,心房水標(biāo)本等等,。本公司提供免費代測服務(wù),，咨詢“人胰島素樣生長因子結(jié)合蛋白4(IGFBP-4 ) "的詳細說明書,。

恒遠生物提供免費代測服務(wù),，產(chǎn)品僅供科研使用,。

產(chǎn)品名稱：人胰島素樣生長因子結(jié)合蛋白4（IGFBP-4 ）

 

英文名稱：Human insulin-like growth factors binding protein 4,IGFBP-4 ELISA KIT

產(chǎn)品規(guī)格：96人份/48人份

主要組成成分：試劑盒

性狀：液體

適應(yīng)癥：科研

貯藏：2-8℃

人胰島素樣生長因子結(jié)合蛋白4（IGFBP-4 ）計算

  以標(biāo)準(zhǔn)物的濃度為橫坐標(biāo),，OD值為縱坐標(biāo)，在坐標(biāo)紙上繪出標(biāo)準(zhǔn)曲線,，根據(jù)樣品的OD值由標(biāo)準(zhǔn)曲線查出相應(yīng)的濃度,；再乘以稀釋倍數(shù),；或用標(biāo)準(zhǔn)物的濃度與OD值計算出標(biāo)準(zhǔn)曲線的直線回歸方程式，將樣品的OD值代入方程式,，計算出樣品濃度,，再乘以稀釋倍數(shù)，即為樣品的實際濃度,。

Steps description（英文）

Standard, Sample diluent

 

Add Standard, Sample diluent, incubate for 30 min at 37℃.

 

Wash 5 time,Add HRP-Conjugate reagent, incubate for 30 min at 37℃.

 

Wash 5 times,Add Chromogen Solution A and B, incubate for 30 min at 37℃.

 

Add Stopp Solution

 

Read absorbance at 450nm within 15 min

 

calculate

Calculate

Take the standard density as the horizontal, the OD value for the vertical ,draw the standard curve on graph paper, Find out the corresponding density according to the sample OD value by the Sample curve, multiplied by the dilution multiple, or calculate the straight line regression equation of the standard curve with the standard density and the OD value ,with the sample OD value in the equation, calculate the sample density, multiplied by the dilution factor, the result is the sample actual density.

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