目錄:北京索萊寶科技有限公司>>細胞相關(guān)產(chǎn)品>>細胞培養(yǎng)試劑>> 10491細胞培養(yǎng) RPMI Medium 1640
| 供貨周期 | 現(xiàn)貨 | 規(guī)格 | 500ml |
|---|---|---|---|
| 貨號 | 11875 | 應(yīng)用領(lǐng)域 | 醫(yī)療衛(wèi)生,化工,生物產(chǎn)業(yè),石油 |
| 主要用途 | 細胞培養(yǎng)試劑 |
細胞培養(yǎng) RPMI Medium 1640
平時研究用的細胞，有需要冷凍保存,。冷凍的過程稱為凍存,，把凍上的細胞化開的過程叫細胞復(fù)蘇。細胞復(fù)蘇是一簡單的試驗操作,，但操作中有許多需要注意的事項,，在實驗操作中注意細小問題，才能使復(fù)蘇后的細胞狀態(tài)保持良好,。
細胞復(fù)蘇實驗準備：
主要器材：一次性滴管,、打火機,、酒精燈、大鑷子,、中鑷子,、記號筆、廢液缸,、封口膜,、剪子。
主要試劑： PBS,、培養(yǎng)液,、消化酶（胰酶）、75%酒精,、雙抗,。
PBS配制：NaCL :4g KCL:0.1g Na2HPO4 :1.745g KH2PO4 :0.1g
三蒸水： 500mL，溶解后高壓滅菌,。
培養(yǎng)基配制：5mL雙抗,、50mL血清,，加入培養(yǎng)基中,。
細胞復(fù)蘇操作步驟：
1.佩戴眼鏡和手套，從液氮罐中取出安瓿或冷凍管,。
2.迅速放入38℃水浴中,，并不時搖動，在1分鐘內(nèi)使其*融化,，然后在無菌下取出細胞,。
3.在1000r/min速度下離心5～10分鐘，棄去上層液,，加入適量培養(yǎng)液后接種于培養(yǎng)瓶中,，接種濃度1×109/L，置37℃溫箱靜置培養(yǎng),，次日更換一次培養(yǎng)液,，繼續(xù)培養(yǎng)，觀察生長情況,。若細胞密度較高,，及時傳代?；驘o需離心直接將細胞加入瓶中,，并加入培養(yǎng)基貼壁培養(yǎng)12～24小時后，充去上清,，換入新鮮培養(yǎng)基繼續(xù)培養(yǎng),。
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細胞培養(yǎng) RPMI Medium 1640
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