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DMEM高糖,，細胞培養(yǎng)

DMEM是現(xiàn)今細胞培養(yǎng)中比較常見的培養(yǎng)基之一，dmem培養(yǎng)基是建立在MEM培養(yǎng)基的基礎上研究出來的,，它與MEM培養(yǎng)基相比較,，DMEM培養(yǎng)基中加大了MEM培養(yǎng)基中各種成分的用量，下文主要介紹一下自己配制DMEM的方法,。

1 制備新鮮三蒸水或Millipore超純水,。

2 稱取所需量的干粉培養(yǎng)基，加入約終體積一半的三蒸水中;若配制一個包裝的培養(yǎng)液,，在將整個包裝的干粉倒入三蒸水后,，需用水洗包裝袋內(nèi)面2次，倒入培養(yǎng)液中,，以保證所有干粉都溶解成培養(yǎng)液,。磁力攪拌或人工攪拌使之*溶解。

3 根據(jù)包裝袋上的要求補加所需量的碳酸氫鈉;根據(jù)實驗需要,，添HEPES(5-20mmol/L),、谷氨酰胺和其他特殊物質。

4 加水定容到終體積,。

5 必要時用1 mol/L 鹽酸和1 mol/L 氫氧化鈉調節(jié)pH。

6 用無菌0.22um濾膜過濾除菌,，分裝于無菌血清瓶中,，4℃冰箱保存。

配制好的培養(yǎng)液用前加入100U/mL青霉素和100U/mL鏈霉素,，并根據(jù)需要加入血清(5%-20%),。 DMEM培養(yǎng)基應用廣泛?；贒MEM培養(yǎng)基中氨基酸,、微生物含量豐富，并具有糖酵解中的丙酮酸和微量Fe離子,，因此在細胞培養(yǎng),、疫苗生產(chǎn)等方面的應用為常見,。

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DMEM高糖,，細胞培養(yǎng)