Z經(jīng)典的RNA提取試劑——MRC公司TRIZOL
具體成交價以合同協(xié)議為準
- 公司名稱 北京嘉美諾斯生物科技有限公司
- 品牌
- 型號
- 產(chǎn)地 http://www.jiamay.com/vshow_21_1301.html
- 廠商性質(zhì) 代理商
- 更新時間 2017/11/26 13:09:38
- 訪問次數(shù) 697
產(chǎn)品標簽
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關(guān)鍵詞:嘉美生物,,實驗技術(shù)服務(wù),Annexin V,,重組蛋白,,磷酸化抗體,各種動物ELISA試劑盒,,RNA干擾,,雙鏈短RNA,TRIZOL,,Northern分析,,RT-PCR

TRI Reagent—zui經(jīng)典的RNA提取試劑,即TRIZOL
☆有效分離RNA,,亦可使用同一系統(tǒng)連續(xù)純化DNA及protein
☆快速簡便,;1小時內(nèi)可得高純度之RNA,且回收率較其它方法高30-150 %
☆本產(chǎn)品為Single Step RNA Isolation技術(shù)創(chuàng)始人Dr. Piotr Chomczynski所研發(fā),,質(zhì)量值得信賴
☆純化之RNA可直接用于Northern分析,,點雜交,分子篩選,體外轉(zhuǎn)染,,RNA酶保護試驗,,分子克隆,和PCR等實驗
☆免DNase處理,,不殘留protein或DNA
☆可用于動,、植物細胞或組織、細菌,、全血,、病毒,酵母菌之液態(tài)或固態(tài)樣品
☆可搭配BCP使用,,取代傳統(tǒng)使用之高毒性氯仿(chloroform)
☆于室溫可保存長達2年,,配方優(yōu)于其它類似試劑。
☆快速簡便,;1小時內(nèi)可得高純度之RNA,且回收率較其它方法高30-150 %
☆本產(chǎn)品為Single Step RNA Isolation技術(shù)創(chuàng)始人Dr. Piotr Chomczynski所研發(fā),,質(zhì)量值得信賴
☆純化之RNA可直接用于Northern分析,,點雜交,分子篩選,體外轉(zhuǎn)染,,RNA酶保護試驗,,分子克隆,和PCR等實驗
☆免DNase處理,,不殘留protein或DNA
☆可用于動,、植物細胞或組織、細菌,、全血,、病毒,酵母菌之液態(tài)或固態(tài)樣品
☆可搭配BCP使用,,取代傳統(tǒng)使用之高毒性氯仿(chloroform)
☆于室溫可保存長達2年,,配方優(yōu)于其它類似試劑。
不同樣本使用TRI Reagent試劑抽提RNA產(chǎn)量 | |
tissue | yield (µg/mg tissue) |
liver | 6月10日 |
spleen | 6月10日 |
kidney | 3月4日 |
skeletal muscle | 1 - 1.5 |
brain | 1 - 1.5 |
placenta | 1月4日 |
cultured cells | yield (µg RNA/106 cells) |
epithelial cells | 8月15日 |
fibroblasts | 5月7日 |
產(chǎn)品訂購信息:
目錄號 | 產(chǎn)品描述 |
TR118 | TRI Reagent(適用于動植物組織,,細胞團和單層生長的細胞) |
TB126 | TRI Reagent BD (適用于血液樣本) |
TS120 | TRI Reagent BD LS (適用于細胞懸浮液和其它液體樣) |
附件:TRIZOL的英文介紹
TRI REAGENT - RNA / DNA / PROTEIN ISOLATION REAGENT Cat. No. TR 118
Store at 4 - 25 C
Store at 4 - 25 C
PRODUCT DESCRIPTION
TRI Reagent? is a complete and ready-to-use reagent for the isolation of total RNA or the simultaneous isolation of RNA, DNA and proteins from samples of human, animal, plant, yeast, bacterial and viral origin. TRI Reagent is the improved version of the popular single-step method of total RNA isolation (1, 2). This highly reliable technique performs well with small and large quantities of tissues or cultured cells and allows simultaneous processing of a large number of samples. TRI Reagent and the single-step method are subjects of the international patents.
TRI Reagent? is a complete and ready-to-use reagent for the isolation of total RNA or the simultaneous isolation of RNA, DNA and proteins from samples of human, animal, plant, yeast, bacterial and viral origin. TRI Reagent is the improved version of the popular single-step method of total RNA isolation (1, 2). This highly reliable technique performs well with small and large quantities of tissues or cultured cells and allows simultaneous processing of a large number of samples. TRI Reagent and the single-step method are subjects of the international patents.
TRI Reagent combines phenol and guanidine thiocyanate in a mono-phase solution to facilitate the immediate and most effective inhibition of RNase activity. A biological sample is homogenized or lysed in TRI Reagent and the homogenate is separated into aqueous and organic phases by bromochloropropane (cat. no. BP 151) or chloroform addition and centrifugation. RNA remains exclusively in the aqueous phase, DNA in the interphase and proteins in the organic phase. RNA is precipitated from the aqueous phase by addition of isopropanol, washed with ethanol and solubilized. DNA and proteins are sequentially precipitated from the interphase and the organic phase with ethanol and isopropanol, washed with ethanol and solubilized.
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