ESGRO和LIF在干細(xì)胞實(shí)驗(yàn)中的優(yōu)點(diǎn)

來源：上海葉舟生物科技有限公司 2012年02月29日 10:05

LIF與ESGRO®用于干細(xì)胞培養(yǎng)的優(yōu)勢(shì)：

1.適合胚胎干細(xì)胞,、神經(jīng)干細(xì)胞、造血干細(xì)胞及間充質(zhì)干細(xì)胞等多種干細(xì)胞培養(yǎng)

2.消除了飼養(yǎng)層細(xì)胞的干擾與污染

3.一致地抑制ES細(xì)胞的分化

4.減少了支原體污染的危險(xiǎn)

5.方便使用的包裝形式

產(chǎn)品名稱	產(chǎn)地	貨號(hào)	規(guī)格
ESGRO® （LIF）	Millipore	ESG1106	1 million units/1 mL
ESGRO® （LIF）	Millipore	ESG1107	10 million units/1 mL
Rat ESGRO®	Millipore	ESG2206	1 million units/1 mL
Rat ESGRO®	Millipore	ESG2207	10 million units/1 mL
Leukemia Inhibitory Factor, recombinant human	Millipore	LIF1005	5UG
Leukemia Inhibitory Factor, recombinant human	Millipore	LIF1010	10UG
Leukemia Inhibitory Factor, recombinant mouse	Millipore	LIF2005	5UG
Leukemia Inhibitory Factor, recombinant mouse	Millipore	LIF2010	10UG
Leukemia Inhibitory Factor, recombinant rat	Millipore	LIF3005	5UG
Leukemia Inhibitory Factor, recombinant rat	Millipore	LIF3010	10UG

Specific Activity:

ESGRO® is assessed both on ES {embryonic stem} cells （D3 & MBL-1） and on murine M1 myeloid leukemic cells（4）. A standard of 50 Units is defined as the concentration of ESGRO®, in 1.0mL of tissue culture medium, that induces the differentiation of 50% of M1 colonies（4）. 10E7 units is equivalent to approximay 100 μg of pure protein, and is sufficient to treat 10L of ES cell culture media.

Embryonic Stem Cell Assay: Differentiation inhibition at 1000 units/mL Murine myeloid leukemic, M1 Assay: Specific Activity >/= 108 units/mg

Promotional Text:

High Quality. Validated. Reliable.

EmbryoMax ESGRO® （LIF）

Description:

ESGRO® （LIF）

Trade Name:

Chemicon （Millipore）

Qty/Pk:107 units

Key Applications:

Stem Cell Culture

Application Notes:

Applications for ESGRO® include use as a reagent for the in vitro maintenance of the pluripotential phenotype of murine ES cells.

SUGGESTED PROTOCOLS:

ES Cells: In D3 and MBL-1 pluripotential ES cell cultures it is routinely found that 1000 U of ESGRO® per 1.0 mL of tissue culture media is required to maintain ES cells with a stem cell phenotype. Similar concentrations of mLIF have also been used for germline transmission of genetically altered ES cells（E3）.

At the recommended concentration 107 units of ESGRO® is sufficient for 10.0 L of tissue culture media and 106 units of ESGRO® is sufficient for 1.0 L of tissue culture media.

Visit www.esgro-lif.com for additional information

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ESGRO和LIF在干細(xì)胞實(shí)驗(yàn)中的優(yōu)點(diǎn)

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