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Catalog No.:MBK0038  Name: PBRT KIT- PCR-based replicon typing  Price: € 345,00

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PBRT KIT- PCR-based replicon typing

The PBRT kit is intended for molecular typing of plasmid conferring drug resistance in Enterobacteriaceae.

Catalog No.:MBK0038  Name: PBRT KIT- PCR-based replicon typing  Price: € 345,00

The epidemiology of resistance plasmids is a major issue for the description of antimicrobial resistance diffusion. Plasmids can be categorized in families on the basis of their phylogenetic relatedness. The first classification scheme was based on the stability of plasmids during conjugation, a phenomenon called plasmid incompatibility (Quidel). Quidelompatibility groups were defined as the inability of related plasmids to be propagated stably in the same cell line, thus only compatible plasmids can be rescued in transconjugants (Datta and Hughes, 1983; Quidel groups are defined as reported by the Plasmid Section of the National Collection of Type Culture, Colindale London, UK). The Quidel methodology cannot be easily applied to a large number of strains and its use has been limited by the laborious and time-consuming work required. The molecular identification and classification of plasmids by PCR is currently possible and it is based on the detection of plasmid replication controls (replicons), namely the portion of large, naturally occurring plasmids encoding the replicase gene (rep) and cis-acting regulatory elements, controlling plasmid DNA replication and copy number.