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在線詢價收藏產(chǎn)品( 聯(lián)系我們,請說明是在 化工儀器網(wǎng) 上看到的信息,,謝謝?。?/p>
產(chǎn)品英文名稱 | Human IGF1 Protein |
產(chǎn)品中文名稱 | 樣生長因子1(IGFI) |
別名 | Long R3 IGF-I; Insulin-like growth factor I; MGF; Somatomedin-C; IBP1 |
標(biāo)簽 | 無標(biāo)簽 |
表達宿主 | 大腸桿菌 |
種屬 | 人 |
序列 | 氨基酸序列來源于:樣生長因子1(IGFI) (P05019-1) (Gly49-Ala118) 表達的蛋白片段。 |
活性 | 通過其誘導(dǎo)MCF-7細胞增殖的能力來測定,。該效應(yīng)的ED50為76.41 ng/mL,。 |
蛋白長度 | 樣生長因子1(IGFI) 由71個氨基酸組成,,預(yù)測分子量為7.8 KD,。 |
純度 | > 99% ,,使用SDS-PAGE檢測 |
特點&優(yōu)勢 | 通過LAL法測定,每微克蛋白里內(nèi)毒素含量<1.0>1.0> |
產(chǎn)品形式 | 凍干粉,,凍干緩沖液為無菌的20 mM Tris,,150 mM NaCl,pH 7.4,。 |
基因ID | 3479 |
蛋白序列鏈接 | /uniprotkb/P05019/entry#P05019-1 |
使用中注意事項 | 開蓋使用前,,請先離心。本產(chǎn)品為凍干粉,,推薦用該產(chǎn)品配套的Reconstitution Buffer進行復(fù)溶,,且濃度不低于100 µg/mL??蛻艨筛鶕?jù)后續(xù)的實驗需求,,進行進一步的稀釋。 |
保存建議 | 本產(chǎn)品為凍干粉形式,,建議:將凍干粉產(chǎn)品保存于-20℃以下的干燥環(huán)境中。本產(chǎn)品溶解之后,,可在4℃保存2-7天,,長期使用請置于-20℃以下。為長期保存,,推薦添加載體蛋白(0.1% HAS/BSA或13%FBS),,并酌情分裝保存,避免反復(fù)凍融,。 |
運輸條件 | 冰袋運輸(藍冰) |
警告 | 本文列出的產(chǎn)品僅供研究使用,,不適用于人類或臨床診斷。我們產(chǎn)品所推薦應(yīng)用,,不是建議使用我們的產(chǎn)品去違反任何或許可證,。對于使用本產(chǎn)品可能發(fā)生的侵權(quán)或其他違規(guī)行為,我們不承擔(dān)任何責(zé)任,。 |
背景 | IGF I, also known as Mechano Growth Factor, somatomedin-C, IGF-I, and IGF1, is a secreted protein that belongs to the insulin family. The insulin family, comprised of insulin, relaxin, insulin-like growth factors I and II ( IGF-I and IGF-II ), and possibly the beta-subunit of 7S nerve growth factor, represents a group of structurally related polypeptides whose biological functions have diverged. The IGFs, or somatomedins, constitute a class of polypeptides that have a key role in pre-adolescent mammalian growth. IGF-I expression is regulated by GH and mediates postnatal growth, while IGF-II appears to be induced by placental lactogen during prenatal development. IGF1 / IGF-I may be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. IGF1 / IGF-I stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also about enhancing glucose uptake. Defects in IGF1 / IGF-I are the cause of insulin-like growth factor I deficiency (IGF1 deficiency) which is an autosomal recessive disorder characterized by growth retardation, sensorineural deafness, and mental retardation. |
Fig.Human IGF1 Protein
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