日韩av大片在线观看欧美成人不卡|午夜先锋看片|中国女人18毛片水多|免费xx高潮喷水|国产大片美女av|丰满老熟妇好大bbbbbbbbbbb|人妻上司四区|japanese人妻少妇乱中文|少妇做爰喷水高潮受不了|美女人妻被颜射的视频,亚洲国产精品久久艾草一,俄罗斯6一一11萝裸体自慰,午夜三级理论在线观看无码

EGF重組蛋白

EGF重組蛋白說(shuō)明書

▍Backgound

Epidermal Growth Factor (EGF) was originally discovered in crude preparations of nerve growth factor prepared from mouse submaxillary glands as an activity that induced early eyelid opening, incisor eruption, hair growth inhibition, and stunting of growth when injected into newborn mice. Human EGF was isolated from urine based on its inhibitory effect on gastric secretion and named urogastrone, accordingly. EGF is prototypic of a family of growth factors that are derived from membrane-anchored precursors. All members of this family are characterized by the presence of at least one EGF structural unit (defined by the presence of a conserved 6 cysteine motif that forms three disulfide bonds) in their extracellular domain. EGF is initially synthesized as a 130 kDa precursor transmembrane protein containing 9 EGF units. The mature soluble EGF sequence corresponds to the EGF unit located proximal to the transmembrane domain. The membrane EGF precursor is capable of binding to the EGF receptor and was reported to be biologically active.

▍Source

Recombinant Human EGF is expressed from human 293 cells (HEK293). It contains AA Asn 971 - Arg 1023. This protein fused with polyhistidine tag at the C terminus. The protein has a calculated MW of 6.2 kDa.

▍Endotoxin

Less than 1.0 EU per μg by the LAL method. 

▍AA Sequence

NSDSECPLSH DGYCLHDGVC MYIEALDKYA CNCVVGYIGE RCQYRDLKWW ELR

▍Purity

Purity >90% as determined by SDS-PAGE.

▍Formulation

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 150mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

▍Reconstitution

Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at < -20°C. Further dilutions should be made in appropriate buffered solutions.

▍Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

▍SDS-PAGE

Human EGF, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.