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當(dāng)前位置:BPS Bioscience Inc.>>PARP Assay Kits>> 78596PARP15-FL Chemiluminescent Assay Kit
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產(chǎn)品型號(hào)78596
品 牌BPS Bioscience
廠商性質(zhì)生產(chǎn)商
所 在 地
更新時(shí)間:2024-11-26 21:38:27瀏覽次數(shù):283次
聯(lián)系我時(shí),,請(qǐng)告知來(lái)自 化工儀器網(wǎng)PARP1 Chemiluminescent Assay Kit (3
The full length PARP15 (PARP15-FL) Chemiluminescent Assay Kit is designed to measure PARP15 activity for screening and profiling applications. PARP15 is known to catalyze the NAD-dependent ADP-ribosylation of histones. This Assay Kit takes advantage of the sensitive detection of biotinylated NAD+. First, histone proteins are coated on a 96-well plate. Next, a biotinylated NAD+ mix (termed PARP Substrate Mixture) is incubated with the PARP15-FL enzyme in an optimized assay buffer. Finally, the plate is treated with streptavidin-HRP followed by addition of the ELISA ECL substrate to produce chemiluminescence that can be measured using a chemiluminescence reader.
Illustration 1: PARP15-FL Chemiluminescent Assay Kit schematic
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