Recombinant HEK293 cell line expressing the full length human CD40 (also known as Tumor necrosis factor receptor superfamily member 5; TNFRSF5). Expression is confirmed by flow cytometry and western blot. An NF-?B luciferase reporter construct is stably integrated into the genome. Thus, the firefly luciferase gene is controlled by 4 copies of an NF-kB response element located upstream of a TATA promoter. Following activation by a CD40 agonist, NF-?B transcription factors bind to the DNA response elements to induce transcription of the luciferase gene. Activation of CD40 is quantified by measuring luciferase activity.

Illustration of CD40/NF-κB Luciferase Reporter HEK293 Cell Line

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