細胞名稱

人侵襲性脈絡膜黑色素瘤細胞MuM-2C             

貨物編碼

BFN608006384

產品規(guī)格

T25培養(yǎng)瓶x1

1.5ml凍存管x2

細胞數(shù)量

1x10^6

1x10^6

保存溫度

37℃

-198℃

運輸方式

常溫保溫運輸

干冰運輸

安全等級

1

用途限制

僅供科研                          2類

培養(yǎng)體系

DMEM高糖培養(yǎng)基（Hyclone）+10%胎牛血清（Gibco）+1%雙抗（Hyclone）

培養(yǎng)溫度

37℃

二氧化碳濃度

5%

簡介

 人侵襲性脈絡膜黑色素瘤細胞MuM-2C取自女性供體,，貼壁培養(yǎng),。倍增時間64h.

注釋

Problematic cell line: Contaminated. Shown to be a M14 derivative (PubMed=28940260). Originally thought to originate from a female patient with metastatic uveal melanoma.

Registration: International Cell Line Authentication Committee, Register of Misidentified Cell Lines; ICLAC-00501.

STR信息

Amelogenin：X；CSF1PO：11,；D13S317：12,；D16S539：9；D18S51：13,，18,；D19S433：14，15,；D21S11：30,；D2S1338：19；D3S1358：14,，16,；D5S818：11，12,；D7S820：8,，10；D8S1179：13,；FGA：21,；TH01：6，7,；TPOX：8,，11；vWA：18,；

參考文獻

PubMed=12067204; DOI=10.1023/A:1015591624171

Seftor E.A., Meltzer P.S., Kirschmann D.A., Pe'er J., Maniotis A.J., Trent J.M., Folberg R., Hendrix M.J.C.

Molecular determinants of human uveal melanoma invasion and metastasis.

Clin. Exp. Metastasis 19:233-246(2002)

PubMed=15299072

Qin J.-Z., Stennett L., Bacon P., Bodner B., Hendrix M.J.C., Seftor R.E.B., Seftor E.A., Margaryan N.V., Pollock P.M., Curtis A., Trent J.M., Bennett F., Miele L., Nickoloff B.J.

p53-independent NOXA induction overcomes apoptotic resistance of malignant melanomas.

Mol. Cancer Ther. 3:895-902(2004)

PubMed=18689700; DOI=10.1167/iovs.08-2324

Folberg R., Kadkol S.S., Frenkel S., Valyi-Nagy K., Jager M.J., Pe'er J., Maniotis A.J.

Authenticating cell lines in ophthalmic research laboratories.

Invest. Ophthalmol. Vis. Sci. 49:4697-4701(2008)

PubMed=22236444; DOI=10.1111/j.1755-148X.2012.00971.x

Griewank K.G., Yu X., Khalili J., Sozen M.M., Stempke-Hale K., Bernatchez C., Wardell S., Bastian B.C., Woodman S.E.

Genetic and molecular characterization of uveal melanoma cell lines.

Pigment Cell Melanoma Res. 25:182-187(2012)

PubMed=28940260; DOI=10.1002/ijc.31067

Korch C., Hall E.M., Dirks W.G., Ewing M., Faries M., Varella-Garcia M., Robinson S., Storts D.R., Turner J.A., Wang Y., Burnett E.C., Healy L., Kniss D., Neve R.M., Nims R.W., Reid Y.A., Robinson W.A., Capes-Davis A.

Authentication of M14 melanoma cell line proves misidentification of MDA-MB-435 breast cancer cell line.

Int. J. Cancer 142:561-572(2017)