細(xì)胞名稱

人黑色素瘤細(xì)胞WM35

貨物編碼

BFN60808633

產(chǎn)品規(guī)格

T25培養(yǎng)瓶x1

1.5ml凍存管x2

細(xì)胞數(shù)量

1x10^6

1x10^6

保存溫度

37℃

-198℃

運(yùn)輸方式

常溫保溫運(yùn)輸

干冰運(yùn)輸

安全等級(jí)

1

用途限制

僅供科研      2類

培養(yǎng)體系

DMEM高糖+10%FBS+1%三抗

培養(yǎng)溫度

37℃

二氧化碳濃度

5%

簡(jiǎn)介

人黑色素瘤細(xì)胞WM35取自24歲女性供體

注釋

Part of: Cancer Cell Line Encyclopedia (CCLE) project.

Part of: COSMIC cell lines project.

Part of: Wistar Institute melanoma cell line collection.

Microsatellite instability: Stable (MSS) (Sanger).

Omics: Deep exome analysis.

Omics: DNA methylation analysis.

Omics: Transcriptome analysis.

Caution: The reported STR profile from Wistar of this cell line was changed at one point between February 2016 when we retrieved them and entered them in the Cellosaurus and May 2018. The major changes were: D18S51: 20->14,15 and FGA: 19->19,24.

基因突變

Heterozygous for BRAF p.Val600Glu (c.1799T>A) (PubMed=18632627; Wistar).

HLA信息

/

STR信息

Amelogenin        X

CSF1PO        10

D2S1338        18,25

D3S1358        16,18

D5S818        11,12

D7S820        10,13

D8S1179        11,13

D13S317        11

D16S539        12,13

D18S51        14,15

D19S433        15,16

D21S11        29,32.2

FGA        19,24

TH01        9.3

TPOX        8

vWA        17

參考文獻(xiàn)

PubMed=23039341; DOI=10.1186/1476-4598-11-75

Byron S.A., Loch D.C., Wellens C.L., Wortmann A., Wu J., Wang J., Nomoto K., Pollock P.M.

Sensitivity to the MEK inhibitor E6201 in melanoma cells is associated with mutant BRAF and wildtype PTEN status.

Mol. Cancer 11:75-75(2012)

PubMed=27087056; DOI=10.1038ep24569

Haridas P., McGovern J.A., Kashyap A.S., McElwain D.L.S., Simpson M.J.

Standard melanoma-associated markers do not identify the MM127 metastatic melanoma cell line.

Sci. Rep. 6:24569-24569(2016)

PubMed=27397505; DOI=10.1016/j.cell.2016.06.017

Iorio F., Knijnenburg T.A., Vis D.J., Bignell G.R., Menden M.P., Schubert M., Aben N., Goncalves E., Barthorpe S., Lightfoot H., Cokelaer T., Greninger P., van Dyk E., Chang H., de Silva H., Heyn H., Deng X., Egan R.K., Liu Q., Mironenko T., Mitropoulos X., Richardson L., Wang J., Zhang T., Moran S., Sayols S., Soleimani M., Tamborero D., Lopez-Bigas N., Ross-Macdonald P., Esteller M., Gray N.S., Haber D.A., Stratton M.R., Benes C.H., Wessels L.F.A., Saez-Rodriguez J., McDermott U., Garnett M.J.

A landscape of pharmacogenomic interactions in cancer.

Cell 166:740-754(2016)

PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747

Dutil J., Chen Z., Monteiro A.N., Teer J.K., Eschrich S.A.

An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.

Cancer Res. 79:1263-1273(2019)