Product No.：

E-007

Product name：

Recombinant SARS-CoV-2 spike protein

 (S1 subunit, 10 x his tag)

Expression host:

HEK293 cells

Protein sequence

VNLTTRTQLPPAYTNSFTRGVYYPDKVFRSSVLHSTQDLFLPFFSNVTWFHAIHVSGTNGTKRFDNPVLPFNDGVYFASTEKSNIIRGWIFGTTLDSKTQSLLIVNNATNVVIKVCEFQFCNDPFLGVYYHKNNKSWMESEFRVYSSANNCTFEYVSQPFLMDLEGKQGNFKNLREFVFKNIDGYFKIYSKHTPINLVRDLPQGFSALEPLVDLPIGINITRFQTLLALHRSYLTPGDSSSGWTAGAAAYYVGYLQPRTFLLKYNENGTITDAVDCALDPLSETKCTLKSFTVEKGIYQTSNFRVQPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGPKKSTNLVKNKCVNFNFNGLTGTGVLTESNKKFLPFQQFGRDIADTTDAVRDPQTLEILDITPCSFGGVSVITPGTNTSNQVAVLYQDVNCTEVPVAIHADQLTPTWRVYSTGSNVFQTRAGCLIGAEHVNNSYECDIPIGAGICASYQTQTNSPRRARAHHHHHHHHHH

Purity

>90% as determined by SDS-PAGE

Figure 1. Purity of S1 (The protein size is larger than expected because of glycosylation)

Biological activity

EC50 = 3.8 nM

Form

The product is supplied as lyophilized powder from sterile PBS, pH 7.4，

5% mannitol,，0.01% tween80

Reconstitution 

Add deionized water to reconstruct the protein to 1 mg/mL.

Stability and Storage

The powder can be stored at 4 °C for up to twelve months. After reconstruction, store under sterile conditions at -20 °C to -80 °C for up to twelve months. It is recommended that the protein is aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.