杭州新喬生物可以提供2種熒光示蹤產(chǎn)品可以用于細胞內(nèi)或活體體內(nèi)乏氧部位的熒光示蹤CY7-NO2
Selective and efficient detection andimaging of nitroreductase (NTR) overexpressed in hypoxic tissues is of great importancefor better understanding its biological functions. The effective optical probesequipped for NTR detection in vivo is still lacking, so we developed aninnovative near infrared (NIR) fluorescent on-off probe (Cy-NO2) composed offluorescence reporting unit (an aminocyaine dye) and recognition unit (p-nitrobenzylcarbamategroup). The response and mechanism of the NTR activated reduction of Cy-NO2were evaluated in vitro through kinetic optical studies, mass spectra analysisand docking calculations. The results demonstrated that Cy-NO2 could rapidlyrecognize NTR with high selectivity and sensitivity. The efficient NTR
detection ability of Cy7-NO2 was furthervalidated by fluorescence imaging of hypoxic cells (A549, PC-12 and HUVECcell). Furthermore, the in vivo hypoxia imaging by Cy-NO2 were evaluated on tumor-bearingmouse,cerebral ischemia (CIS) and deep vein thrombosis models. The resultsindicated a rapid and distinct enhancement of its NIR fluorescence highlyappropriate for in vivomonitoring of NTR in all of the three animal models,whichrevealed aspiring clinical value of this NIR fluorescent hypoxia probe.
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