染料修飾的脫氧尿苷5'-三磷酸鹽(如氨基烯丙基dUTP)可用于通過常規(guī)酶摻入方法(如逆轉錄、缺口翻譯,、隨機引物標記或PCR)生產含染料的DNA,。這種酶熒光標記方法廣泛用于FISH探針和基于微陣列的實驗,。這種Cy3-dUTP綴合物可以用Spectrum Orange™濾光片組作為紅色熒光色(Spectrum Oranges™是Vysis的商標),。它在功能上與Cyanine 3-dUTP(Perkin-Elmer的NEL578001EA)相當,。
艾美捷花青-3- dUTP [Cy 3-dUTP] :
貨號 AAT-17025
單位大?。?span style="font-family: Calibri;">25納摩爾
分子量:1150.00
溶劑:水
預期用途 僅供研究使用(RUO)
儲存條件:冷凍(<-15°C),;盡量減少光照
校正因子(260納米):0.07
校正因子(280納米):0.073
消光系數(cm -1 M -1):150000
激發(fā)(納米):555
發(fā)射(納米):569
量子產率:0.15
圖:Cy3和iFluor®555 dUTP標記的端粒探針在中期HeLa細胞中的熒光原位雜交,。
花青-3- dUTP [Cy 3-dUTP] 文獻參考:
A Fluorescent DNA Hydrogel Aptasensor Based on the Self-Assembly of Rolling Circle Amplification Products for Sensitive Detection of Ochratoxin A.
Authors: Hao, Liling and Wang, Wei and Shen, Xueqing and Wang, Shuliu and Li, Qian and An, Faliang and Wu, Shijia
Journal: Journal of agricultural and food chemistry (2020): 369-375
Engineering nicking enzymes that preferentially nick 5-methylcytosine-modified DNA.
Authors: Gutjahr, Alice and Xu, Shuang-yong
Journal: Nucleic acids research (2014): e77
Digital detection of multiple minority mutants in stool DNA for noninvasive colorectal cancer diagnosis.
Authors: Deng, Lili and Qi, Zongtai and Zou, Binjie and Wu, Haiping and Huang, Huan and Kajiyama, Tomoharu and Kambara, Hideki and Zhou, Guohua
Journal: Analytical chemistry (2012): 5645-52
Digital analysis of the expression levels of multiple colorectal cancer-related genes by multiplexed digital-PCR coupled with hydrogel bead-array.
Authors: Qi, Zongtai and Ma, Yinjiao and Deng, Lili and Wu, Haiping and Zhou, Guohua and Kajiyama, Tomoharu and Kambara, Hideki
Journal: The Analyst (2011): 2252-9
Microisolation and microcloning of bovine X-chromosomes for identification of sorted buffalo (Bubalus bubalis) spermatozoa.
Authors: Zhuang, Xin-jie and Lu, Yang-qing and Zhang, Ming and Lu, Sheng-sheng and Lu, Ke-huan
Journal: Animal reproduction science (2011): 32-6
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