CD68巨噬細(xì)胞（鼠單克隆抗體）

廣州健侖生物科技有限公司

CD68 是一種分子量為110KDa 的糖蛋白,，與溶酶體顆粒有關(guān),。此抗體可研究人體許多組織中的巨噬細(xì)胞,，包括脾臟,、腸固有層,、肺泡和骨髓中的枯否細(xì)胞和巨噬細(xì)胞,，外周血的單核細(xì)胞也呈陽性反應(yīng)，同時(shí)也可以研究骨髓前體細(xì)胞和外周血粒細(xì)胞,。近年來有文獻(xiàn)報(bào)道,，CD68也可以作為組織細(xì)胞的標(biāo)志物。主要用于巨噬細(xì)胞方面的研究以及急慢性髓樣白血病和組織細(xì)胞來源的腫瘤如惡性纖維組織細(xì)胞瘤的研究。

我司還提供其它進(jìn)口或國產(chǎn)試劑盒：登革熱,、瘧疾,、流感、A鏈球菌,、合胞病毒,、腮病毒、乙腦,、寨卡,、黃熱病、基孔肯雅熱,、克錐蟲病,、違禁品濫用、肺炎球菌,、軍團(tuán)菌,、化妝品檢測、食品安全檢測等試劑盒以及日本生研細(xì)菌分型診斷血清,、德國SiFin診斷血清,、丹麥SSI診斷血清等產(chǎn)品。

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【產(chǎn)品介紹】

細(xì)胞定位：細(xì)胞漿/細(xì)胞膜

克隆號(hào)：KP-1

同型：IgG1/K

適用組織：石蠟/冰凍

陽性對(duì)照：扁桃體

抗原修復(fù)：熱修復(fù)（檸檬酸）

抗體孵育時(shí)間：30-60min

CD68巨噬細(xì)胞（鼠單克隆抗體）

經(jīng)Percoll密度梯度離心,，細(xì)胞主要在相鄰梯度的界面處形成細(xì)胞帶,。如表2所示，分布在11%～19%Percoll梯度間的主要是細(xì)胞間質(zhì),、細(xì)胞碎片及少量死細(xì)胞,，記為1帶；分布在19%～27%percoll梯度間的細(xì)胞較少,，平均密度為2.35×105個(gè)／ml,，記為2帶；分布在27%～35% Percoll梯度間的細(xì)胞zui多,，平均密度達(dá)1.58×106,，記為3帶；分布在35%～43% Percoll梯度間的細(xì)胞也較少,，平均密度為2.68×105,，記為4帶。統(tǒng)計(jì)學(xué)分析顯示，2、3,、4帶中細(xì)胞分布差異極顯著(P<0.01)，其中3帶與2,、4帶之間相比差異極為顯著(P<0.01),，2、4帶之間相比差異不顯著(P>0.05),。
3.分離細(xì)胞的形態(tài)學(xué)鑒定
電鏡觀察顯示,，分離細(xì)胞圓形或卵圓形，胞質(zhì)中除可見有少量具有板層狀嵴的線粒體外其他細(xì)胞器不發(fā)達(dá),；核質(zhì)比較大,，核大呈圓形，常染色質(zhì)細(xì)密均勻,，異染色質(zhì)少,，呈塊狀附著于核膜內(nèi)面或散在于核內(nèi)。其形態(tài)結(jié)構(gòu)特征與7～8d小鼠睪丸切片上的精原細(xì)胞**(圖1),。
4.精原細(xì)胞的分離純度
1帶中主要為死細(xì)胞及細(xì)胞碎片,；2、4帶中細(xì)胞接種后,，大部分細(xì)胞延展后伸出突起,，從形態(tài)學(xué)判定，主要為支持細(xì)胞及少量管周肌樣細(xì)胞,。主要收集3帶中細(xì)胞,，接種培養(yǎng)約3～4h，其中的支持細(xì)胞開始貼壁而精原細(xì)胞尚未貼壁,，此時(shí)進(jìn)行精原細(xì)胞的純化；純化細(xì)胞體外培養(yǎng)約7～8h后,，絕大部分支持細(xì)胞已貼壁,，大部分精原細(xì)胞開始貼壁，此時(shí)進(jìn)行細(xì)胞計(jì)數(shù),。結(jié)果如表4所示,，3帶中精原細(xì)胞的純度平均達(dá)到68.76%。

我司還提供其它進(jìn)口或國產(chǎn)試劑盒：登革熱,、瘧疾,、流感、A鏈球菌,、合胞病毒,、腮病毒、乙腦,、寨卡,、黃熱病、基孔肯雅熱,、克錐蟲病,、違禁品濫用,、肺炎球菌、軍團(tuán)菌,、化妝品檢測,、食品安全檢測等試劑盒以及日本生研細(xì)菌分型診斷血清、德國SiFin診斷血清,、丹麥SSI診斷血清等產(chǎn)品,。

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【公司名稱】 廣州健侖生物科技有限公司
【市場部】     歐

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【騰訊  】 
【公司地址】 廣州清華科技園創(chuàng)新基地番禺石樓鎮(zhèn)創(chuàng)啟路63號(hào)二期2幢101-103室

After Percoll density gradient centrifugation, the cells formed cell strands predominantly at adjacent gradient interfaces. As shown in Table 2, interstitial cells, cell debris and a small number of dead cells distributed between 11% and 19% of Percoll gradient were recorded as a band. Fewer cells distributed between 19% and 27% percoll gradients, The average density was 2.35 × 105 cells / ml, which was recorded as 2 bands. The cells distributed in 27% -35% Percoll gradient had the highest average density of 1.58 × 106, which was recorded as 3 bands. The distribution was between 35% and 43% of Percoll gradient Between the cells less, the average density of 2.68 × 105, recorded as 4 band. Statistical analysis showed that there was significant difference in the distribution of cells in 2,3,4 band (P <0.01), among which the difference between 3 band and 2,4 band was extremely significant (P <0.01) Compared with the difference was not significant (P> 0.05).
3. Isolation of cells morphological identification
Electron microscopy showed that the cells isolated round or oval, in addition to the cytoplasm can be seen in a small amount of mitochondria with lamellar ridges other underdeveloped organelles; relatively large nuclear, nuclear large circular, dense chromatin, Heterochromatin less, was attached to the nucleus inside the patch or scattered in the nucleus. The morphological features of the spermatogonia were consistent with the spermatogonia in 7-8 day mouse testis sections (Figure 1).
Separation of spermatogonia purity
1 with dead cells and cell debris in the band; 2,4 cells with inoculation, the majority of cells after the extension of the protruding process, from the morphological determination, mainly for supporting cells and a small amount of tubular muscle-like cells. Mainly collected cells with 3, inoculation culture of about 3 ~ 4h, in which the supporting cells began to adhere to the spermatogonia have not yet attached (Figure 2), then spermatogonia purified; purified cells cultured in vitro about 7 ~ 8h After most of the supporting cells have been adherent, most of the spermatogonia began to attach (Figure 3), at this time for cell counting. As shown in Table 4, the purity of the spermatogonia in the third band reached an average of 68.76%.