CD3(T細(xì)胞)兔單克隆抗體

廣州健侖生物科技有限公司

CD3是一種能直接與T細(xì)胞抗原受體(TCR)相連的蛋白質(zhì)復(fù)合物,，與TCR組成復(fù)合受體分子,，是T細(xì)胞識(shí)別抗原的主要識(shí)別單位，具有穩(wěn)定TCR 結(jié)構(gòu)和傳遞活化信號(hào)的作用,，是幼稚和成熟T細(xì)胞的的標(biāo)志之一,。此抗體可用于T細(xì)胞淋巴瘤的研究。

我司還提供其它進(jìn)口或國(guó)產(chǎn)試劑盒：登革熱,、瘧疾,、流感、A鏈球菌,、合胞病毒,、腮病毒、乙腦,、寨卡,、黃熱病、基孔肯雅熱,、克錐蟲病,、違禁品濫用、肺炎球菌,、軍團(tuán)菌,、化妝品檢測(cè)、食品安全檢測(cè)等試劑盒以及日本生研細(xì)菌分型診斷血清,、德國(guó)SiFin診斷血清,、丹麥SSI診斷血清等產(chǎn)品。

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【產(chǎn)品介紹】

細(xì)胞定位：細(xì)胞膜

克隆號(hào)：MRQ-39

同型：IgG

適用組織：石蠟/冰凍

陽(yáng)性對(duì)照：扁桃體

抗原修復(fù)：熱修復(fù)（EDTA）

抗體孵育時(shí)間：30-60min

CD3(T細(xì)胞)兔單克隆抗體

2,、細(xì)胞大小的測(cè)定
⑴將酵母菌斜面制成一定濃度的菌懸液（10－2）。
⑵取一滴酵母菌菌懸液制成水浸片,。
⑶移去鏡臺(tái)測(cè)微尺,，換上酵母菌水浸片，先在低倍鏡下找到目的物,，然后在高倍鏡下用目鏡測(cè)微尺來(lái)測(cè)量酵母菌菌體的長(zhǎng),，寬各占幾格,，不足一格的部分估計(jì)到小數(shù)點(diǎn)后一位數(shù),。測(cè)出的格數(shù)乘上目鏡測(cè)微尺每格的校正值，即等于該菌的長(zhǎng)和寬,。一般測(cè)量菌體的大小要在同一個(gè)標(biāo)本片上測(cè)定10～20個(gè)菌體,，求出平均值,，才能代表該菌的大小。待測(cè)微生物需用培養(yǎng)至對(duì)數(shù)生長(zhǎng)期的菌體進(jìn)行測(cè)定,。
⑷同樣方法用油鏡測(cè)定枯草桿菌染色標(biāo)本的細(xì)胞大小,。
（二）微生物細(xì)胞的顯微直接計(jì)數(shù)法
1、視待測(cè)菌懸液濃度,，加無(wú)菌水適量稀釋（斜面一般稀釋到10－2）,，以每小格的菌數(shù)可數(shù)為度。
2,、取潔凈的血球計(jì)數(shù)板一塊,，在計(jì)數(shù)區(qū)上蓋上一塊蓋玻片。
3,、將酵母菌懸液搖勻,，用滴管吸取少許，從計(jì)數(shù)板中間平臺(tái)兩側(cè)的溝槽內(nèi)沿蓋玻片的下邊緣滴入一小滴,，不宜過(guò)多,，讓菌懸液利用液體的表面張力充滿計(jì)數(shù)區(qū)，勿使氣泡產(chǎn)生,，并用吸水紙吸去溝槽中流出的多余菌懸液,。也可以將菌懸液直接滴加在計(jì)數(shù)區(qū)上，注意不要使計(jì)數(shù)區(qū)兩邊平臺(tái)沾上菌懸液,，以免加蓋蓋玻片后,，造成計(jì)數(shù)區(qū)深度的升高。然后加蓋蓋玻片,，勿使產(chǎn)生氣泡,。
4、靜置片刻,，將血球計(jì)數(shù)板置載物臺(tái)上夾穩(wěn),，先在低倍鏡下觀察到計(jì)數(shù)區(qū)后，再轉(zhuǎn)換高倍鏡觀察并計(jì)數(shù),。由于活細(xì)胞的折光率和水的折光率相近,，觀察時(shí)應(yīng)適當(dāng)關(guān)小孔徑光闌并減弱光照的強(qiáng)度。
5,、計(jì)數(shù)時(shí)若計(jì)數(shù)區(qū)是由16個(gè)大方格組成,，按對(duì)角線方位，數(shù)左上,、左下,、右上、右下的4個(gè)大方格（即100小格）的菌數(shù),。如果是25個(gè)大方格組成的計(jì)數(shù)區(qū),，除數(shù)上述四個(gè)大方格外,，還需數(shù)中央1個(gè)大方格的菌數(shù)（即80個(gè)小格）。如菌體位于大方格的雙線上,，計(jì)數(shù)時(shí)則數(shù)上線不數(shù)下線,，數(shù)左線不數(shù)右線，以減少誤差,。
6,、對(duì)于出芽的酵母菌，芽體達(dá)到母細(xì)胞大小一半時(shí),，即可作為兩個(gè)菌體計(jì)算,。每個(gè)樣品重復(fù)計(jì)數(shù)2～3次，每次數(shù)值不應(yīng)相差過(guò)大,，否則應(yīng)重新操作,，求出每一個(gè)小格中細(xì)胞平均數(shù)（N），按公式計(jì)算出每mL(g)菌懸液所含酵母菌細(xì)胞數(shù)量,。
7,、測(cè)數(shù)完畢，取下蓋玻片,，用水將血球計(jì)數(shù)板沖洗干凈,，切勿用硬物洗刷或抹擦，以免損壞網(wǎng)格刻度,。洗凈后自行晾干或用吹風(fēng)機(jī)吹干,，放入盒內(nèi)保存。

CD3(T細(xì)胞)兔單克隆抗體

我司還提供其它進(jìn)口或國(guó)產(chǎn)試劑盒：登革熱,、瘧疾,、流感、A鏈球菌,、合胞病毒,、腮病毒、乙腦,、寨卡,、黃熱病、基孔肯雅熱,、克錐蟲病,、違禁品濫用、肺炎球菌,、軍團(tuán)菌,、化妝品檢測(cè)、食品安全檢測(cè)等試劑盒以及日本生研細(xì)菌分型診斷血清、德國(guó)SiFin診斷血清,、丹麥SSI診斷血清等產(chǎn)品。

想了解更多的產(chǎn)品及服務(wù)請(qǐng)掃描下方二維碼：

【公司名稱】 廣州健侖生物科技有限公司
【市場(chǎng)部】    楊永漢

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【騰訊  】 
【公司地址】 廣州清華科技園創(chuàng)新基地番禺石樓鎮(zhèn)創(chuàng)啟路63號(hào)二期2幢101-103室

2, the determination of cell size
⑴ yeast slant made a certain concentration of bacterial suspension (10-2).
⑵ take a drop of yeast suspension made of water immersion tablets.
⑶ remove the mirror micrometer scale, put the yeast immersion film, first find the object at low magnification, and then under high magnification with eyepiece micrometer to measure the length and width of yeast cells, each of several grid , Less than a single part of the estimated number of decimal places. Multiply the number of cells measured by each eyepiece micrometer ruler calibration, that is equal to the length and width of the bacteria. General measurement of the size of the cells in the same specimen measured 10 to 20 cells, to find the average, in order to represent the size of the bacteria. The microorganisms to be tested need to be cultured to the logarithmic growth phase.
⑷ the same method with the oil microscope to determine the cell size of B. subtilis stained specimens.
(B) Micro-cell direct micro-counting method
1, depending on the concentration of bacterial suspension test, add appropriate amount of sterile water dilution (beveled generally diluted to 10-2), in order to count the number of bacteria per cell for the degree.
2, take a clean blood count plate, covered in a count area cover glass.
3 shake the suspension of yeast, with a little pipette, counting plate from the middle of the groove on both sides of the cover along the bottom edge of a small drop, not too much, so that the bacterial suspension of liquid Of the surface tension is full of counting area, do not make bubbles, and use suction paper to absorb excess bacteria in the trench suspension. The bacterial suspension can also be dropped directly on the counting area, be careful not to make the platform on both sides of the counting area stained with bacterial suspension, so as not to cover the cover glass, resulting in increased count zone depth. Then cover with glass, do not make bubbles.
4, let it stand for a moment, the hemocytometer plate set on stage stationary, first observed under low magnification count area, and then converted to observe and count high magnification. Due to the refractive index of living cells and water similar to the observation should be appropriate off aperture aperture and reduce the intensity of light.
5, counting the count area is composed of 16 large square, according to the diagonal line, the number of upper left, lower left, upper right, lower right of the four large grid (ie 100 small cell) bacteria. If it is a counting area composed of 25 large squares, in addition to the above four large squares, it also needs to count the number of bacteria in one large square in the center (ie, 80 small squares). Such as bacteria located in the large double-line grid, count the number of on-line countless off the assembly line, the number of left-right line on the right to reduce the error.
6, budding yeast, bud body to half the size of the mother cell, you can calculate as two cell body. Repeat counting for 2 ~ 3 times for each sample, each value should not differ too much, or should be re-operated to calculate the average number of cells (N) in each cell, according to the formula to calculate the bacterial suspension Yeast cells contained in the number.
7, the test is completed, remove the coverslip, water to wash the blood count plate, do not use hard objects to scrub or wipe, so as not to damage the grid scale. Wash yourself or dry with a hair dryer, into the box to save.