Enzo Life Sciences的CYTO-ID®綠色/紅色長期細胞示蹤試劑盒采用專有的非共價細胞標(biāo)記技術(shù),，將含有疏水性脂肪鏈的熒光染料穩(wěn)定地結(jié)合入細胞膜的脂質(zhì)雙分子層中。標(biāo)記緩沖液對哺乳動物細胞是等滲的,，不含去污劑或有機溶劑,。染料對細胞沒有毒性，可在細胞中保留長達96小時,，并在有絲分裂時傳遞給子細胞,。由于該染料不對細胞內(nèi)的蛋白質(zhì)進行共價修飾，與基于硫醇反應(yīng)的氯甲基或胺反應(yīng)的琥珀酰亞胺酯類熒光染料的分子探針相比,，細胞正常的生理反應(yīng)得到更好的保留,。

也可以使用CELLESTIAL®染料進行雙重標(biāo)記。標(biāo)記的細胞可以通過共聚焦熒光顯微鏡等儀器進行觀察,。此外,，染料標(biāo)記的和未標(biāo)記的細胞群可通過流式細胞儀進行分析。在延長96小時的培養(yǎng)后,，沒有觀察到熒光轉(zhuǎn)移到相鄰細胞,。這與Calcein AM和BCECF AM標(biāo)記形成鮮明對比，后者在生理溫度下只能在存活的細胞內(nèi)保留幾個小時,。

Enzo Life Sciences的CYTO-ID®長期細胞示蹤試劑盒適用于多種應(yīng)用,，如追蹤細胞譜系，長期細胞活力,、細胞毒性,、細胞粘附、細胞遷移和細胞-細胞融合研究,。

產(chǎn)品特點

● 可使用各種CELLESTIAL® 熒光探針進行雙重標(biāo)記

● 熒光從染料標(biāo)記的細胞轉(zhuǎn)移到未標(biāo)記的細胞的情況極少

● 適用于長期細胞存活率,、細胞毒性、細胞粘附、細胞遷移和細胞間融合測定

實驗示例

圖1.明場（A）和熒光顯微鏡圖像（B）顯示了用CYTO-ID® Green Tracer dye對Jurkat細胞的染色,。使用標(biāo)準(zhǔn)的FITC（綠色）濾光片組對膜結(jié)合信號進行成像,。

圖2.流式細胞術(shù)分析Jurkat細胞混合群隨時間變化的熒光。將用 CYTO-ID®綠色示蹤染料染色的Jurkat細胞與未染色的Jurkat細胞群混合并孵育120小時,。

圖3.明場（A）和熒光顯微鏡圖像（B）顯示了用CYTO-ID® Red Tracer dye對Jurkat細胞的染色,。使用標(biāo)準(zhǔn)的Texas Red（紅色）濾光片組對膜結(jié)合信號進行成像。

圖4.熒光顯微鏡圖像顯示了用CYTO-ID® Red Tracer dye對HeLa細胞的染色,。使用標(biāo)準(zhǔn)的Texas Red（紅色）濾光片組對膜結(jié)合信號進行成像(20x),。

圖5.流式細胞術(shù)分析Jurkat細胞混合群隨時間變化的熒光。將用 CYTO-ID®紅色示蹤染料染色的Jurkat細胞與未染色的Jurkat細胞群混合并孵育96小時,。

產(chǎn)品信息

部分產(chǎn)品引用文獻

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2. A liquid biopsy-based method for the detection and quantification of circulating tumor cells in surgical osteosarcoma patients: H. Zhang , et al.; Int. J. Oncol. 50, 1075 (2017), Application(s): Use with HOS cells (osteosarcoma). Microscopy

3. INBRX-120, a CD8α-targeted detuned IL-2 that selectively expands and activates tumoricidal effector cells for safe and durable in vivo responses: F. J. Sulzmaier, et al.; J. Immunother. Cancer 11, e006116 (2023)

4. Epigenetic modulation of immune synaptic-cytoskeletal networks potentiates γδ T cell-mediated cytotoxicity in lung cancer: R.R. Weng, et al.; Nat. Commun. 12, 2163 (2021)

5. Characterization and Cellular Internalization of Spherical Cellulose Nanocrystals (CNC) into Normal and Cancerous Fibroblasts: N.A.H. Shazali, et al.; Materials (Basel) 12, 3251 (2019), Application(s): C6 and NIH3T3 cell tracing

6. Time resolved 3D live-cell imaging on implants: A. Ingendoh-Tsakmakidis, et al.; PLoS One 13, e0205411 (2018)

7. Nerve guidance conduit with a hybrid structure of a PLGA microfibrousbundle wrapped in a micro/nanostructured membrane: S.W. Peng, et al.; Int. J. Nanomedicine 12, 421 (2017), Application(s):Tracking in vitro cells migration (immortalized neuron progenitor KT 98)

8. Thioreductase-containing epitopes inhibit the development of type 1 diabetes in the NOD mouse model: E. Malek Abrahimians, et al.; Front. Immunol. 7, 67 (2016), Application(s): Flow cytometry analysis using isolated mouse spleen B cells

9. Syngeneic murine ovarian cancer model reveals that ascites enriches for ovarian cancer stem-like cells expressing membrane GRP78: L. Mo, et al.; Mol. Cancer Ther. 14, 747 (2015) 

10. An in vitro blood-brain barrier model combining shear stress and endothelial cell/astrocyte co-culture: Y. Takeshita, et al.; J. Neurosci. Methods 232, 165 (2014), Application(s): Confocal microscopy using adult human astrocyte cells

11. Vascular endothelial growth factor-C modulates proliferation and chemoresistance in acute myeloid leukemic cells through an endothelin-1-dependent induction of cyclooxygenase-2: K.T. Hua, et al.; Biochim. Biophys. Acta 1843, 387 (2014)

12. Expanding the Ig superfamily code for laminar specificity in retina: expression and role of contactins: M. Yamagata, et al.; J. Neurosci. 32, 14402 (2012)

詳情請聯(lián)系Enzo Life Sciences金牌代理——欣博盛生物