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上海橋星貿(mào)易有限公司
主營(yíng)產(chǎn)品： 進(jìn)口透析袋,密理博ECL發(fā)光液,B27無(wú)血清培養(yǎng)基,Gibco膠原酶 |
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上海橋星貿(mào)易有限公司
主營(yíng)產(chǎn)品： 進(jìn)口透析袋,密理博ECL發(fā)光液,B27無(wú)血清培養(yǎng)基,Gibco膠原酶 |
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M3005 Go-Taq 500u Promega試劑
原裝*,，質(zhì)量保證,，現(xiàn)貨供應(yīng)
規(guī)格：500U
M3005 Go-Taq 500u Promega試劑 產(chǎn)品描述：
組分：
GoTaq® DNA Polymerase【子貨號(hào)：M300B，包裝：1 x 500u,，,，運(yùn)保溫度：-20℃】
5X Green GoTaq® Reaction Buffer【子貨號(hào)：M791A，包裝：4 x 1ml,，,，運(yùn)保溫度：-20℃】
5X Colorless GoTaq® Reaction Buffer【子貨號(hào)：M792A，包裝：4 x 1ml,，,，運(yùn)保溫度：-20℃】
描述：
說(shuō)明 | GoTaq® DNA 聚合酶類產(chǎn)品能夠增強(qiáng)聚合酶鏈?zhǔn)椒磻?yīng)(PCR)性能。GoTaq® DNA 聚合酶是一種有配方的Taq DNA 聚合酶,，性能強(qiáng)勁,，擴(kuò)增效果至少等同于或優(yōu)于普通Taq DNA 聚合酶。GoTaq® DNA 聚合酶類產(chǎn)品有多種配方,，可提供zui大限度的靈活性,、可控性以及方便性。 GoTaq® Flexi DNA Polymerase(GoTaq® Flexi DNA 聚合酶)可對(duì)PCR 反應(yīng)中的酶和鎂離子的濃度進(jìn)行優(yōu)化,。所提供的5X 綠色及無(wú)色Flexi 反應(yīng)緩沖液中不包含鎂離子,。MgCl2 (25mM) 單獨(dú)提供，可實(shí)現(xiàn)對(duì)反應(yīng)條件的zui大程度的控制,。MgCl2 也可單獨(dú)提供,。
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特點(diǎn) | • 提高性能：這種新的緩沖液及酶的配方使得PCR 反應(yīng)的性能得以改善,。通過(guò)使用GoTaq®Flexi 反應(yīng)緩沖液，也可以摸索反應(yīng)中的*Mg2+ 濃度,。 • 提高生產(chǎn)效率：直接從PCR 反應(yīng)進(jìn)入凝膠分析,。綠色GoTaq®反應(yīng)緩沖液既是反應(yīng)緩沖液，又是凝膠加樣溶液,。• 不需要改變循環(huán)條件：可將GoTaq® 酶( 不論是無(wú)色的,，還是綠色的) 直接應(yīng)用于您現(xiàn)有的PCR 反應(yīng)，不需要改變?cè)瓉?lái)的循環(huán)參數(shù),。 • 與PCR增強(qiáng)劑兼容：GoTaq® DNA 聚合酶可與甜菜堿及DMSO等PCR增強(qiáng)劑兼容,；兩者都不會(huì)影響GoTaq® 綠色反應(yīng)緩沖液的顏色或特性。 • 快速便捷：GoTaq® Green Master Mix 與PCR Master Mix 提供極大的便利——可在不到一分鐘內(nèi)組裝好反應(yīng)體系,，只要加入模板,、水和引物就可以開(kāi)始了。 |
原廠資料：
Experience improved PCR performance with GoTaq® DNA Polymerase products. GoTaq® DNA Polymerase is a proprietary formulation of Taq DNA polymerase that gives robust amplification equal to and in some cases superior to that of standard Taq DNA polymerase. GoTaq® DNA Polymerase comes in a variety of formulations to give you maximum flexibility, control and convenience. GoTaq® Hot Start Polymerase is also available.
GoTaq® Flexi DNA Polymerase allows you to optimize enzyme and magnesium concentration in your PCR. The supplied 5X Green and Colorless Flexi Reaction Buffers do not contain magnesium. A separate tube of 25mM MgCl2 is supplied, giving you maximum control over your reaction conditions. MgCl2 is also available separay.
GoTaq® DNA Polymerase provides improved amplification with the convenience of reaction buffers containing magnesium. The 5X GoTaq® Green and Colorless Reaction Buffers supplied with GoTaq® DNA Polymerase contain MgCl2 at a concentration of 7.5mM, giving a final concentration of 1.5mM in the 1X reaction.
The 5X Green and 5X Colorless Reaction Buffers supplied with GoTaq® enzymes allow you to go directly from thermal cycler to gel analysis. These buffers contain a compound that increases sample density, so that samples sink easily into wells of an agarose gel. The green buffer also contains two dyes (yellow and blue) that separate to allow easy monitoring during electrophoresis. The blue dye comigrates at the same rate as 3–5kb DNA fragments in a 1% agarose gel. The yellow dye migrates ahead of primers (<50bp). Use the green reaction buffer for direct-to-gel analysis after amplification and the colorless reaction buffer for post-amplification analysis by fluorescence or absorbance without prior DNA purification.
For ultimate convenience, choose GoTaq® Colorless Master Mix or GoTaq® Green Master Mix. Both are premixed, ready-to-use 2X solutions containing GoTaq® DNA Polymerase, dNTPs, MgCl2 and reaction buffers at optimal concentrations for efficient amplification of DNA templates by PCR. GoTaq® Green Master Mix also includes two dyes (blue and yellow) that allow monitoring of progress during electrophoresis. GoTaq® Colorless Master Mix has the same formulation as the GoTaq® Green Master Mix but does not include the dyes. Both also include Nuclease-Free Water. Reactions assembled with the GoTaq® Master Mixes have sufficient density for direct loading onto agarose gels.
PCR Satisfaction Guarantee
Promega's PCR Systems, enzymes and reagents are proven in PCR to ensure reliable, high performance results. Your success is important to us. Our products are backed by a worldwide team of Technical Support scientists. Please contact them for applications or technical assistance. If you are not compley satisfied with any Promega PCR product we will send a replacement or refund your account.That's Our PCR Guarantee!
Product must be within expiration date and have been stored and used in accordance with product literature. See Promega Product Insert for specific tests preformed.
For more information, see the Protocols & Applications Guide.
GoTaq® Green and Colorless Master Mix reaction size is based on a 50µl reaction volume.
M3005 Go-Taq 500u Promega試劑 相關(guān)產(chǎn)品列表：
| Promega 系列 | ||
| 序號(hào) | 貨號(hào) 名稱 | 規(guī)格 |
| 1 | M1801 T4連接酶 | 100U |
| 2 | M1804 T4連接酶 | 500U |
| 3 | M1701 M-MLV | 10000U |
| 4 | M1705 M-MLV | 50000U |
| 5 | M5101 AMV | 300U |
| 6 | M5108 AMV | 1000U |
| 7 | M7741 Pfu | 100U |
| 8 | M7745 Pfu | 500U |
| 9 | C1101 Olig(dt)15 Primer | 20ug |
| 10 | M3001 Go-Taq | 100U |
| 11 | M3005 Go-Taq | 500U |
| 12 | M3008 Go-Taq | 2500U |
| 13 | U1511 dNTP | 200μl |
| 14 | U1515 dNTP | 1000μl |
| 15 | A1360 pGEM-T easy | 1.2ug |
| 16 | A3600 pGEM-T | 1.2ug |
| 17 | C1181 Random Primers | 20μg |
| 18 | N2511 Recombinant RNasin | 2500U |
| 19 | N2515 Recombinant RNasin | 10000U |
| 20 | N2611 RNasin Plus | 2500U |
| 21 | N2615 RNasin Plus | 10000U |
| 22 | N2111 RNasin | 2500U |
| 23 | N2115 RNasin | 10000U |
| 24 | M1871 TdT | 300U |
| 25 | M1875 TdT | 1500U |
| 26 | M2825 CIAP | 1000U |
| 27 | M1821 CIAP | 1000U |
| 28 | M2201 Klenow I | 150U |
| 29 | M2206 Klenow I | 500U |
| 30 | M4211 T4 DNA Polymerase | 100U |
| 31 | M4215 T4 DNA Polymerase | 500U |
| 32 | M4101 T4 Polynucleotide Kinase | 100U |
| 33 | M4103 T4 Polynucleotide Kinase | 1000U |
