Recombinant Ectonucleotide Pyrophosphatase/Phosphodiesterase 2 (ENPP2)  

外核苷酸焦磷酸酶/磷酸二酯酶2(ENPP2)重組蛋白

[ PROPERTIES ]

Residues: Arg637~Lys905,with two N-terminal Tags, His-tag and T7-tag

Host:E. coli

Subcellular Location: Secreted

Purity: >95%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation:Supplied as lyophilized form in 20mM Tris,150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,0.01% sarcosyl, 5% trehalose, and preservative.

Predicted isoelectric point: 6.5

Predicted Molecular Mass: 35.0kDa

Applications:SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in ddH₂O.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

           Store at 2-8oC for one month.

           Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

[SEQUENCE ]

The target protein is fused with two N-terminal Tags, His-tag and T7-tag, itssequence is listed below.

MGSSHHHHHH SSG LVPRGSH MASMTGGQM GRGSEF -RLHT KGSTEERH LLYGRPAVLYRT RYDILYHTDF ESGYSEIFLM PLWTSYTVSK QAEVSSVPDH LTSCVRPDVRVSPSFSQNCL AYKNDKQMSY GFLFPPYLSS SPEAKYDAFL VTNMVPMYPA FKRVWNYFQRVLVKKYASER NGVNVISGPI FDYDYDGLHD TEDKIKQYVE GSSIPVPTHY YSIITSCLDFTQPADKCDGP LSVSSFILPH RPDNEESCNS SEDESKWVEE LMKMHTARVRDIEHLTSLDFFRKTSRSYPE ILTLK

[  IDENTIFICATION  ]

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