Recombinant Tubulin Beta 3 (TUBb3)

微管蛋白β3(TUBb3)重組蛋白

[ PROPERTIES ]

Source:Prokaryotic expression

Host: E.coli

Residues:Tyr222~Glu438

Tags:Two N-terminal Tags, His-tag and GST-tag

Tissue Specificity: Lung, Brain, Breast Cancer.

Subcellular Location: Cytoplasm, cytoskeleton.

Purity: > 92%

Traits:Freeze-dried powder

Buffer formulation: PBS, pH7.4, containing 1mM DTT, 5%trehalose, 0.01%sarcosyl and Proclin300.

Original Concentration:200µg/mL

Applications:SDS-PAGE; WB; ELISA; IP; CoIP; Purification; Amine ReactiveLabeling.

(May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point:6.1

Predicted Molecular Mass:54.7kDa

Accurate Molecular Mass:54kDa as determined by SDS-PAGE reducingconditions.

[ USAGE ]

Reconstitute in PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

           Store at 2-8oC for one month.

           Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

[SEQUENCE ]

[  IDENTIFICATION  ]

[ IMPORTANT NOTE ]

The kit is designed for research use only, we will not be responsible for any issue if the kit was used in clinical diagnostic or any other procedures.

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