Recombinant Phosphoinositide-3-Kinase Adaptor Protein 1 (PIK3AP1)  

磷酸肌醇-3-激酶銜接蛋白1(PIK3AP1)重組蛋白

[ PROPERTIES ]

Residues: Leu489~Ser730,with two N-terminal Tags, His-tag and T7-tag.

Host:E. coli

Subcellular Location: Cytoplasm. Cellmembrane; Peripheral membrane protein.

Purity: >95%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation:Supplied as lyophilized form in PBS,pH7.4, containing 5% trehalose, 0.01% sarcosyl.

Predicted isoelectric point: 9.1

Predicted Molecular Mass: 31.5kDa

Applications:SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

           Store at 2-8oC for one month.

           Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

[SEQUENCE ]

The target protein is fused with two N-terminal Tags, His-tag and T7-tag, itssequence is listed below.

MGSS HHHHHH SSG LVP RGS H MASM TG GQM GRGSEF - LE GNSMGMT N LERDQCHLGQEE DVYHTVDDDE AFSVDLASRP PVPVPRPETT APGAHQLPDNEPYIFKVFAEKSQERPGNFY VSSESIRKGP PVRPWRDRPQ SSIYDPFAGM KTPGQRQLIT LQEQVKLGIVNVDEAVLHFK EWQLNQKKRS ESFRFQQENL KRLRDSITRR QREKQKSGKQTDLEITVPIRHSQHLPAKVE FGVYESGPRK SVIPPRTELR RGDWKTDSTS STASSTSNRS

[  IDENTIFICATION  ]

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