Recombinant Phosphoenolpyruvate Carboxykinase 2, Mitochondrial (PCK2)

磷酸烯醇式丙酮酸羧激酶2(PCK2)重組蛋白

[ PROPERTIES ]

Source:Prokaryotic expression

Host: E.coli

Residues: Leu33~Val198

Tags:N-terminal His Tag

Subcellular Location: Mitochondrion

Purity: > 99%

Traits:Freeze-dried powder

Buffer formulation: 100mM NaHCO₃, 500mM NaCl, pH8.3, containing1mMEDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300.

Original Concentration:200µg/mL

Applications:SDS-PAGE; WB; ELISA; IP; CoIP; Purification; Amine ReactiveLabeling.

(May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point:7.9

Predicted Molecular Mass: 21.8kDa

Accurate Molecular Mass: 24kDa as determined by SDS-PAGE reducingconditions.

[ USAGE ]

Reconstitute in 100mM NaHCO3, 500mM NaCl (pH8.3) to a concentrationof0.1-1.0 mg/mL. Do not vortex.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

           Store at 2-8oC for one month.

           Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

[SEQUENCE ]

[  IDENTIFICATION  ]

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