BDCA2 Effector Reporter Cell IO細(xì)胞株 BDCA2 Effector Reporter Cell IO細(xì)胞株 BDCA2 Effector Reporter Cell IO細(xì)胞株
| I. Background | |
| 血小板樹(shù)突狀細(xì)胞抗原 2(BDCA2)是一種僅在人 pDC 表面表達(dá)的C型凝集素,。BDCA2 由一個(gè)糖基識(shí)別的胞外域,、一個(gè)跨膜區(qū)和一個(gè)短的不含任何信號(hào)轉(zhuǎn)導(dǎo)結(jié)構(gòu)域的胞內(nèi)段組 成。BDCA-2與跨膜區(qū)的 FcRγ鏈形成 adaptor 來(lái)傳遞細(xì)胞內(nèi)信號(hào),，并誘導(dǎo)類似 B 細(xì)胞受體 (BCR)的信號(hào)轉(zhuǎn)導(dǎo)級(jí)聯(lián)反應(yīng),。 抗體介導(dǎo)的 BDCA2 結(jié)合會(huì)導(dǎo)致 SYK 招募到 FcεRIγ磷酸化的免疫受體 基激活結(jié)構(gòu)域(ITAM),。SYK 激活會(huì)導(dǎo)致 BTK 和 PLCγ2 激活，從而導(dǎo)致鈣離子動(dòng)員,。BDCA2 受體的結(jié)合已被證明可以抑制 TLR7 或 TLR9 誘導(dǎo)的 IFN I 和其他 pDC 衍生的促炎介質(zhì),。 | |
| II. Description | |
BDCA2 Effector Reporter 報(bào)告基因藥靶模型很好的模擬了體內(nèi) BDCA2 的信號(hào)轉(zhuǎn)導(dǎo)過(guò)程， 原理見(jiàn)下圖 所示,。 Figure 1. BDCA2 Effector Reporter 細(xì)胞模型原理圖 | |
| III. Introduction | |
| Expressed gene: | BDCA2 |
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | RPMI-1640+10%FBS+1ug/ml puromycin+800ug/ml hygromycin+5ug/ml blasticidin, |
| Mycoplasma Testing: | Negative |
| Storage: | Liquid nitrogen |
| Application(s): | Functional(Report Gene) Assay |
IV. Representative Data | |
Figure 2. Recombinant BDCA2 Effector Reporter Cell stably expressing BDCA2.
Figure 3. Dose Response of Litifilimab in BDCA2 Effector Reporter Cells (C12).
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