| I. Background | |
| cAMP 反應(yīng)元件結(jié)合蛋白 (CREB) 位于細(xì)胞核內(nèi)，是一種轉(zhuǎn)錄因子,，在 Ser133 位點(diǎn)被不同的受體活化蛋白激酶（如蛋白激酶 A (PKA),、鈣調(diào)蛋白依賴性蛋白激酶 (CaMK)、絲裂原活化蛋白激酶 (MAPK) 和其他激酶磷酸化后,，與靶基因啟動(dòng)子的 cAMP 反應(yīng)元件 (CRE)結(jié)合,。CRE 是 CREB 的主要結(jié)合位點(diǎn)，負(fù)責(zé)激活 CREB,。CRE 是許多細(xì)胞外和細(xì)胞內(nèi)信號(hào)通路的焦點(diǎn),，包括 cAMP、鈣,、GPCR（G 蛋白偶聯(lián)受體）和神經(jīng)營(yíng)養(yǎng)因子,。cAMP/PKA 信號(hào)通路對(duì)許多生命過程和生物體至關(guān)重要。在 cAMP/PKA 信號(hào)通路中,，CREB 通過 PKA 的磷酸化被激活,，并與 CRE 結(jié)合,，一般基序?yàn)?5'-TGACGTCA-3'。由于 CRE 是 cAMP/PKA信號(hào)通路的調(diào)節(jié)劑,，因此可以研究各種抑制劑的作用,。 | |
| II. Description | |
| CRE-Luc HEK293 報(bào)告基因細(xì)胞是由 CRE 調(diào)控并表達(dá) Luc 熒光素酶報(bào)告基因的 HEK293 細(xì)胞。Forskolin 是天然存在的直接腺苷酸環(huán)化酶（AC）/cAMP/CREB 的 激活劑,，原理見下圖所示,。
Figure 1：CRE-Luc HEK293 原理示意圖 | |
| III. Introduction | |
| Host Cell: | HEK293 |
| Expressed gene: | CRE-Luciferase |
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Synonym(s): | CRE cell line, CREB cell line, luciferase reporter |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | DMEM+10%FBS+150ug/ml hygromycin |
| Mycoplasma Testing: | Negative |
| Storage: | Liquid nitrogen |
| Application(s): | • Monitor cAMP/PKA signaling pathway activity. • Screen for activators or inhibitors of the cAMP/PKA signaling pathway. |
| IV. Description of Host Cell Line | |
| Organism: | Homo sapiens, human |
| Tissue: | Embryonic kidney |
| Disease: | Normal |
| Morphology: | Epitheloid cell |
| Growth Properties: | Adherent |
| Ⅴ. Representative Data | |
| |
Figure 2. Detect Luciferase assay by Ultra Luciferase Detection Kit CBPH0001（we strongly suggest to purchase from Cobioer). Induction of CRE activity by Forskolin in CRE-Luc HEK293(C5). | |
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