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[供應]Origen/Wak/Sigma/MP二甲基亞砜DMS及DMSO-DEX40中國*總代理
DMSO-DEX40用于血液凍存,。
Origen/Wak/Sigma/MP二甲基亞砜DMS及DMSO-DEX40中國*總代理
德國WAK、美國Origen中國代理商
DMSO 屬于非質子極性溶劑,,常用于化學反應,、 PCR反應以及在細胞、組織和器官的保存中用作玻璃化低溫冷凍防護劑,。 DMSO用于細胞冷凍培養(yǎng)基內,可以保護細胞免受冰晶引起的機械性損傷,。它也可以用于主培養(yǎng),、亞培養(yǎng)、重組異倍體、雜交瘤等系列細胞株,,胚胎干細胞( ESC)和造血干細胞的冷凍保藏,。另外常常與 BSA或FBS混合使用。
用 途:
(1) DMSO廣泛應用在人,、動物細胞株及細菌噬菌體λ的低溫防護中,,制備 DMSO溶液用來冷凍細胞的步驟如下:
A.配制冷凍培養(yǎng)基:含有細胞培養(yǎng)用培養(yǎng)基、 10-20% 血清和 5-10% DMSO,;
B.用胰酶或其他合適的方法消化貼壁細胞,;(細胞處于對數(shù)增長期)
C.低速離心(4℃,250×g, 10min)收集細胞,,吸去培養(yǎng)基,;
D.用冷凍培養(yǎng)基懸浮細胞,使細胞密度為 106 -107 cells/ml,;
E.平均分配細胞,,裝入凍存管內;
F.依照標準凍存程序冷凍細胞,,凍存在 -70℃或更低的溫度內,;
(2)細胞融合時,40-50% PEG 溶液內加入10% DMSO,;
(3)有文獻報道 DMSO作為緩沖液的成分,,用于以下的實驗:Poly(A+)RNA 的純化, E. coli感受態(tài)細胞轉化,、 PCR反應,、 cDNA文庫的擴增、 DNA測序,、 DEAE-葡聚糖介導的細胞轉染,、聚凝胺介導的 DNA轉染;
(4)有相應的程序描述到用 DMSO回收膜過濾后的 DNA,,用于接下來的 PCR擴增反應,;
(5)用于 DNA測序的毛細管電泳技術將 5% DMSO (v/w)混入 2 M尿素中,如果需要可以調整 DMSO濃度到*,。
(6)有研究報道不同濃度的 DMSO與寡核苷酸的熔點溫度關系,;
(7)有研究描述利用 DMSO提高雜交瘤細胞中單克隆抗體的產(chǎn)量; 調查發(fā)現(xiàn)低溫冷凍前用含有 DMSO的培養(yǎng)基高溫培養(yǎng)雜交瘤細胞,。
(8)文獻報道利用 DMSO修飾蛋白中的磷酸絲氨酸和磷酸蘇氨酸殘基用作磷酸化狀態(tài)的質譜分析( MS),;
(9)文獻報道水和 DMSO中的亮脯利特(Leuprolide)降解作用;
互溶性:
? *互溶: LDPE, HDPE,,聚丙烯 , PPCO, 聚甲基戊烯,,尼龍, teflon FEP
? 中度互溶:聚苯乙烯, ECTFE/ETFE
? 不 互 溶:聚砜 ,,, flexible and rigid PVC tubing,,聚碳酸脂
注意事項:
僅用于實驗室,不可用于藥品,、家居和其他用處 ,。
使用說明:
DMSO 易于水混合( 1 ml DMSO + 1 ml H2O)形成清澈、無色的溶液,。易吸潮,,免暴露在潮濕環(huán)境。
儲存 /穩(wěn)定性:
DMSO 容易過冷并且室溫下緩慢融化,,收到的產(chǎn)品應為固體并非液體狀態(tài),。固化的 DMSO放在室溫下可以再次液化,對其本身無損害,。屬于熱穩(wěn)定化合物,,在高達 100℃的酸、堿及中性溶液中保持穩(wěn)定,。溫度接近其沸點(189℃),,在堿性和中性溶液中仍保持穩(wěn)定。 150℃加熱 24h,,其純度損失小于0.1%,。
要制備濾膜除菌的 DMSO溶液,推薦使用 teflon或 nylon膜,,盡量不用醋酸纖維素膜,。
產(chǎn)品基本信息:
一、德國WAK中國總代
1.CryoSure-DMSO Quantity Cat #
10 ml Multi-dose 10/case WAK-DMSO-10
50 ml Multi-dose 6/case WAK-DMSO-50
70 ml Multi-dose 6/case WAK-DMSO-70
2.CryoSure-DEX40 Quantity Cat #
8 ml Multi-dose 25/case WAK-DEX40-25
50 ml Multi-dose 5/case WAK-DEX40-50
二,、美國Origen中國總代
1,、CryoPur DMSO
OriGen manufactures sterile filtered CryoPurTM * DMSO and CryoPur-DTM DMSO/Dextran.
The solutions are packaged in syringes or vials. Both packages are shatter resistant. All syringes are shipped in a tray to prevent any damage while in transit, and are fitted with a stopcock, shipped with the actuator blocked for safety.
Our DMSO products are CE marked and certified USP and Pharmacopeia Europa Standards.
VIALS
CryoPurTM: * DMSO
Stock Number Product Description Stock Item European Reference Number
CP-05 * DMSO, 5ml Fill Volum V10005
CP-10 * DMSO, 10ml Fill Volume X V10010
CP-50 * DMSO, 50ml Fill Volume X V10050
CP-70 * DMSO, 70ml Fill Volume X V10070
CP-100 * DMSO, 100ml Fill Volume X V10100
CryoPur-DTM: 55% w/v DMSO, Buffered with 5% w/v Dextran-40
(*CryoPur-D is a mixture of pure DMSO, Dextran-40 and Water for Injection, with the resulting solution being 55% w/v DMSO, 5% Dextran-40, and the remainder water for injection)
Stock Number Product Description Stock Item European Reference Number
CD-05 DMSO/Dextran/remainder water, 5ml fill volume
CD-07 DMSO/Dextran/remainder water, 7.2ml fill volume X V504007
CD-35 DMSO/Dextran/remainder water, 35ml fill volume X V504035
CD-50 DMSO/Dextran/remainder water, 50ml fill volume X V504050
CD-70 DMSO/Dextran/remainder water, 70ml fill volume V504070
CD-100 DMSO/Dextran/remainder water, 100ml fill volume X V5040100
CryoPur-STM: 55% w/v DMSO, Buffered with 5% w/v Dextran-40
(*CryoPur-S is a mixture of pure DMSO, Dextran-40 and remainder 0.72% Saline, with the resulting solution being 55% w/v DMSO, 5% Dextran-40, and the remainder saline.)
Stock Number Product Description Stock Item European Reference Number
CDS-50B DMSO/Dextran/saline, 50ml fill volume V204850-S7
CDS-100B DMSO/Dextran/saline, 100ml fill volume V2048100-S7
SYRINGES
CryoPurTM: * DMSO
Stock Number Product Description Stock Item European Reference Number
SP-10 * DMSO, 10ml in 20ml syringe, stopcock and SCD tubing X S10010
SP-20 * DMSO, 20ml in 20ml syringe, stopcock and SCD tubing X
SP-30 * DMSO, 30ml in 60ml syringe, stopcock and SCD tubing X S10030
SP-50 * DMSO, 50ml in 60ml syringe, stopcock and SCD tubing X S10050
SP-10-1 * DMSO, 10ml in 20ml syringe, stopcock and cap S10010SC
SP-50-1 * DMSO, 50ml in 60ml syringe, stopcock and cap S10050SC
CryoPur-DTM: 55% w/v DMSO, Buffered with 5% w/v Dextran-40
(*CryoPur-D is a mixture of pure DMSO, Dextran-40 and Water for Injection, with the resulting solution being 55% w/v DMSO, 5% Dextran-40, and the remainder water for injection)
Stock Number Product Description Stock Item European Reference Number
SD-05 DMSO/Dextran/remainder water, 5ml in 20ml syringe, stopcock and SCD Tubing S504005
SD-06 DMSO/Dextran/remainder water, 6ml in 20ml syringe, stopcock and SCD Tubing S504006
SD-07 DMSO/Dextran/remainder water, 7.2ml in 20ml syringe, stopcock and SCD Tubing X S504007
SD-10 DMSO/Dextran/remainder water, 10ml in 20ml syringe, stopcock and SCD Tubing X S504010
SD-12 DMSO/Dextran/remainder water, 12ml in 20ml syringe, stopcock and SCD Tubing S504012
SD-10-1 DMSO/Dextran/remainder water, 10ml in 20ml syringe, stopcock and cap S504010SC
CryoPur-STM: 55% w/v DMSO, Buffered with 5% w/v Dextran-40
(*CryoPur-S is a mixture of pure DMSO, Dextran-40 and remainder 0.9% Saline, with the resulting solution being 55% w/v DMSO, 5% Dextran-40, and the remainder saline.)
Stock Number Product Description Stock Item European Reference Number
SDS-05A-B DMSO/Dextran/saline, 5ml fill volume + 2ml air in 20ml syringe, stopcock and SCD Tubing
SDS-15A DMSO/Dextran/saline, 15ml in 20ml syringe, stopcock and SCD tubing SDS-20A DMSO/Dextran/saline, 20ml in 20ml syringe, stopcock and SCD tubing
CryoPurTM: 80% DMSO, remainder water for injection
Stock Number Product Description Stock Item European Reference Number
SPW-10C DMSO, remainder water, 10ml in 20ml syringe, stopcock and SCD tubing SPW-30C DMSO, remainder water, 30ml in 60ml syringe, stopcock and SCD tubing SPW-50C DMSO, remainder water, 50ml in 60ml syringe, stopcock and SCD tubing
Storage
* DMSO: +20 to 30°C (freezes/solidifies at 18°C, DMSO/Dextran: +2 to 8°C
Packaging
10ml vials: 12 to a case, larger vials: 6 to a case, syringes sold individually
Custom Sizes
Custom packages and formulations are possible, please enquire. Minimum order volumes may apply. 8-12 week lead time on custom items.
USA: For research use only.
三、美國SIGMA
中文名(CN) 英文名(EN) CAS No. 規(guī)格(SP) 產(chǎn)地(From) 類別(Key)
二甲基亞砜 (常規(guī)) Dimethyl sulfoxide (≥99.5%, DMSO) 67-68-5 500ML Sigma-D5879
二甲基亞砜 (常規(guī)) Dimethyl sulfoxide (≥99.5%, DMSO) 67-68-5 4L Sigma-D5879
二甲基亞砜 (常規(guī)) Dimethyl sulfoxide (≥99.5%, DMSO) 67-68-5 20L Sigma-D5879二甲基亞砜 (細胞級) Dimethyl sulfoxide (≥99.5%,cell culture) 67-68-5 100ML Sigma-D4540
二甲基亞砜 (細胞級) Dimethyl sulfoxide (≥99.5%,cell culture) 67-68-5 500ML Sigma-D4540
二甲基亞砜 (Hybri-Max) Dimethyl sulfoxide (≥99.7%, Hybri-Max) 67-68-5 5X10ML Sigma-D2650
二甲基亞砜 (Hybri-Max) Dimethyl sulfoxide (≥99.7%, Hybri-Max) 67-68-5 100ML Sigma-D2650
二甲基亞砜 (USP級) Dimethyl sulfoxide (≥99.9%, USP Grade) 67-68-5 500ML Sigma-D1435
二甲基亞砜 (分子生物級) Dimethyl sulfoxide (for molecular biology,≥99.9%) 67-68-5 100ML Sigma-D8418
四,、美國MPBio
貨號 名稱 規(guī)格
196055 DMSO (二甲基亞砜)100ml MPbio
其他產(chǎn)品:
10mL CryoSure-DMSO USP級凍存液 10mL/瓶 WAK-Chemie WAK-DMSO-10-Bag
50mL CryoSure-DMSO USP級凍存液 50mL/瓶 WAK-Chemie WAK-DMSO-50-Bag
70mL CryoSure-DMSO USP級凍存液 70mL/瓶 WAK-Chemie WAK-DMSO-70-Bag
1010mL CryoSure-DMSO USP級凍存液 1010mL/瓶 WAK-Chemie WAK-DMSO-10
6x50mL CryoSure-DMSO USP級凍存液 6x50mL/瓶 WAK-Chemie WAK-DMSO-50
6x70mL CryoSure-DMSO USP級凍存液 6x70mL/瓶 WAK-Chemie WAK-DMSO-70
CryoSure-DEX40 DMSO和右旋糖酐細胞凍存混合液 25x8mL/盒 WAK-Chemie WAK-DEX40-25
CryoSure-DEX40 DMSO和右旋糖酐細胞凍存混合液 5x50mL/盒 WAK-Chemie WAK-DEX40-50-5
50mL DMSO 二甲基亞砜,,EP,USP級凍存液 50mL/瓶 Sigma-Aldrich D2438-50ML
5x10mL DMSO 二甲基亞砜,EP,USP級凍存液 20100mL/瓶 Sigma-Aldrich D2438-5X10ML
100mL DMSO 二甲基亞砜,,純度≥99.5%,,科研級 100mL/瓶 Sigma-Aldrich D4540-100ML
500mL DMSO 二甲基亞砜,純度≥99.5%,,科研級 500mL/瓶 Sigma-Aldrich D4540-500ML
1000mL DMSO 二甲基亞砜,,純度≥99.5%,科研級 1000mL/瓶 Sigma-Aldrich D4540-1L
10mL CryoPur-DMSO CE認證 USP級凍存液 10mL/瓶 OriGen BioMedical CP10-Bag
70mL CryoPur-DMSO CE認證 USP級凍存液 70mL/瓶 OriGen BioMedical CP70-Bag
100mL CryoPur-DMSO CE認證 USP級凍存液 100mL/瓶 OriGen BioMedical CP100-Bag
10mL CryoPur-DMSO CE認證 USP級凍存液 12x10mL/瓶 OriGen BioMedical CP10
70mL CryoPur-DMSO CE認證 USP級凍存液 6x70mL/瓶 OriGen BioMedical CP70
100mL CryoPur-DMSO CE認證 USP級凍存液 6x100mL/瓶 OriGen BioMedical CP100
Cryostore:Freezing Bags Freeze volumemin-max,10-30mL 凍存袋 24袋/箱 OriGen BioMedical CS50
Cryostore:Freezing Bags Freeze volumemin-max,30-70mL 凍存袋 24袋/箱 OriGen BioMedical CS250
歡迎各位新老客戶,,,,訂購?。?nbsp;
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