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產品英文名稱：HEK 293 HCP ELISA kit
產品中文名稱：HEK 293 細胞 ELISA 試劑盒
HEK 293 HCP ELISA kit 的應用
本試劑盒是用于確定在HEK 293細胞表達產品中宿主細胞蛋白污染的存在。該試劑盒是用于研究和制造使用，不用于人類或動物的診斷應用,。這是HEK HCP檢測第二代ELISA試劑盒。這種新的試劑盒，取代原來的F150 ELISA試劑盒，不再是市售,。第二代測定使用一個更廣泛的反應性抗體,。這樣的試劑盒可以產生與使用舊F150試劑盒不同的HEK HCP污染水平的報告。
HEK 293 細胞 ELISA 試劑盒的英文描述
Cygnus is pleased to announce the re-introduction of HEK 293 Host Cell Protein ELISA kit. This 2nd Generation kit utilizes antibodies derived from the same pool of goat anti-HEK antiserum used in the 1st Generation kit, however this antiserum has now been affinity purified with new and a more represenatitve preparation of HCPs typically found in virus, vaccine and recombinant protein products.
HEK 293 HCP ELISA kit 的使用步驟規(guī)則
The HEK assay is a two-site immunoenzymetric assay. Samples containing HEK HCPs are reacted simultaneously with a horseradish peroxidase (HRP) enzyme labeled anti-HEK antibody (goat polyclonal) in microtiter strips coated with an affinity purified capture anti-HEK antibody. The immunological reactions result in the formation of a sandwich complex of solid phase antibody-HCP-enzyme labeled antibody. The microtiter strips are washed to remove any unbound reactants. The substrate, tetramethyl benzidine (TMB) is then reacted. The amount of hydrolyzed substrate is read on a microtiter plate reader and is directly proportional to the concentration of HEK HCPs present.
HEK 293 HCP ELISA kit 的保存和穩(wěn)定性
* All reagents should be stored at 2°C to 8°C for stability until the expiration date printed.
* The substrate reagent should not be used if its stopped absorbance at 450nm is greater than 0.1.
* Reconstituted wash solution is stable until the expiration date of the kit.
HEK 293 HCP ELISA kit 的使用注意事項
*只用于研究或制造。
*停止反應試劑是0.5N H2SO4,。避免接觸眼睛,，皮膚,，和服裝。在用本試劑盒的試劑濃度,，其他沒有被認為是有害的,。
*本試劑盒只能由合格的技術人員使用,。
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