Mini Plasmid Kit采用超順磁性磁珠和優(yōu)化的緩沖液體系,,可方便,、快捷地從大腸桿菌中提取超螺旋質(zhì)粒。提取過(guò)程采用磁性分離,,菌體裂解后不需要通過(guò)高速離心去除雜質(zhì),,有利于實(shí)現(xiàn)高通量操作。提取的質(zhì)??捎糜诿盖小y(cè)序,、PCR等后續(xù)操作,。
產(chǎn)品簡(jiǎn)介
詳細(xì)介紹
質(zhì)粒提取磁珠試劑盒
Mini Plasmid Kit采用超順磁性磁珠和優(yōu)化的緩沖液體系,可方便,、快捷地從大腸桿菌中提取超螺旋質(zhì)粒,。提取過(guò)程采用磁性分離,菌體裂解后不需要通過(guò)高速離心去除雜質(zhì),,有利于實(shí)現(xiàn)高通量操作,。提取的質(zhì)粒可用于酶切,、測(cè)序,、PCR等后續(xù)操作。
產(chǎn)品信息
- 操作簡(jiǎn)單:菌體裂解后無(wú)需離心或抽濾,;
- 輕松實(shí)現(xiàn)高通量:適用于在96孔板中操作,;
- 產(chǎn)物純度高:A 260/280=1.7~2.0。
應(yīng)用實(shí)例
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Agarose gel analysis of plasmids. Plasmid 1~Plasmid 4 were propagated in DH5α. Plasmid 5~Plasmid 7 were propagated in BL21(DE3) pLysS, *0 and JM109, respectively. M: Thermo Scientific GeneRuler 1kb DNA Ladder (# SM0311) A: Plasmid extracted by BeaverBeadsTM Mini Plasmid Kit (without centrifugation) B: Plasmid extracted by BeaverBeadsTM after centrifugation C: Plasmid extracted by spin column after centrifugation purifie |
如上圖所示,,采用本產(chǎn)品提取的質(zhì)粒DNA,,純度及超螺旋的構(gòu)象都能達(dá)到傳統(tǒng)競(jìng)爭(zhēng)產(chǎn)品的水平,由于提取過(guò)程中采用磁珠法去除裂解液中的雜質(zhì)(A),,質(zhì)粒DNA得率會(huì)略低于離心后再提取的樣品(B,、C),但整個(gè)提取過(guò)程避免了高速離心步驟,,有利于實(shí)現(xiàn)高通量及自動(dòng)化操作,。 |