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翌圣明星CP--轉染試劑與PCR產品又登《Cell》期刊

2022-3-26  閱讀(508)

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翌圣明星CP--轉染試劑與PCR產品又登《Cell》期刊


2022年3月17日,,西湖大學生命科學學院陶亮團隊《Cell》期刊(IF:41.582)在線發(fā)表了題為《TFPI is a colonic crypt receptor for TcdB from hypervirulent clade 2 C. difficile》的研究論文,。該研究對于揭示和理解艱難梭菌感染的致病機制,,梭菌毒素的進化和工作方式有著重要科學意義,,并為艱難梭菌感染的預防和治療提供了重要的理論依據,。


1648102171535128.png

圖片來源:論文截圖


團隊在該研究中選擇了翌圣的轉染試劑與PCR產品用于相關基因的研究:

1648102191487797.png
圖片來源:論文截圖

目前轉染試劑系列與PCR系列的產品已經榮登《Nature》《Cell》等多個頂級期刊,,獲得科研大牛們認可,!

以下僅展示部分論文:

轉染試劑系列:


向下滑動查看


[1] Liu R, Yang J, et al. Optogenetic control of RNA function and metabolism using engineered light-switchable RNA-binding proteins. Nat Biotechnol. 2022 Jan 3. (IF 55)華東理工大學生物工程學院楊弋團隊

[2] Zhou J, Chen P, et al. Cas12a variants designed for lower genome-wide off-target effect through stringent PAM recognition. Mol Ther. 2022 Jan 5.(IF 11.454)武漢大學生命科學學院殷雷團隊

[3] Chen S, Cao X, et al. circVAMP3 Drives CAPRIN1 Phase Separation and Inhibits Hepatocellular Carcinoma by Suppressing c-Myc Translation. Adv Sci (Weinh). 2022 Jan 24.(IF 16.808)中國科學院北京生命科學研究院趙方慶團隊

[4] Gu C, Wang Y, et al. AHSA1 is a promising therapeutic target for cellular proliferation and proteasome inhibitor resistance in multiple myeloma. J Exp Clin Cancer Res. 2022 Jan 6.(IF 11.161)南京中醫(yī)藥大學楊燁顧春艷團隊

[5] Zhang Y, Yu X, et al. Splicing factor arginine/serine-rich 8 promotes multiple myeloma malignancy and bone lesion through alternative splicing of CACYBP and exosome-based cellular communication. Clin Transl Med. 2022 Feb.(IF 11.492)南京中醫(yī)藥大學楊燁顧春艷團隊

[6] Qin J, Cai Y, et al. Molecular mechanism of agonism and inverse agonism in ghrelin receptor. Nat Commun. 2022 Jan 13.(IF 14.9)四川大學生物治療國家重點實驗室邵振華團隊

[7] Tang X, Deng Z, et al. A novel protein encoded by circHNRNPU promotes multiple myeloma progression by regulating the bone marrow microenvironment and alternative splicing. J Exp Clin Cancer Res. 2022 Mar 8.(IF 11.161)南京中醫(yī)藥大學楊燁顧春艷團隊

[8] Xie F, Su P, et al. Engineering Extracellular Vesicles Enriched with Palmitoylated ACE2 as 19 Therapy. Adv Mater. 2021 Oct 19. (IF 30.849)蘇州大學生物醫(yī)學研究院周芳芳團隊和浙江大學生命科學研究院張龍團隊

[9] Liang Y, Lu Q, et al. Reactivation of tumour suppressor in breast cancer by enhancer switching through NamiRNA network. Nucleic Acids Res. 2021 Sep 7.(IF 16.9)復旦大學生物醫(yī)學研究院于文強團隊

[10] Fan Y, Wang J, et al. CircNR3C2 promotes HRD1-mediated tumor-suppressive effect via sponging miR-513a-3p in triple-negative breast cancer. Mol Cancer. 2021 Feb 2.(IF 27.403)南京醫(yī)科大學附屬逸夫醫(yī)院蘇東明團隊

[11] Dai L, Dai Y, et al. Structural insight into BRCA1-BARD1 complex recruitment to damaged chromatin. Mol Cell. 2021 Jul 1.(IF 17.97)浙江大學生命科學研究院黃俊團隊和中科院生物物理所周政團隊

[12] Zhang K, Wang A, et al. UBQLN2-HSP70 axis reduces poly-Gly-Ala aggregates and alleviates behavioral defects in the C9ORF72 animal model. Neuron. 2021 Jun 16.(IF 17.17)中國科學院生物與化學交叉研究中心王文元團隊

[13] Li T, Chen X, et al. A synthetic BRET-based optogenetic device for pulsatile transgene expression enabling glucose homeostasis in mice. Nat Commun. 2021 Jan 27.(IF 14.92)華東理工大學生物工程學院楊弋團隊

[14] Pan Y, He X, et al. Neuronal activity recruits the CRTC1/CREB axis to drive transcription-dependent autophagy for maintaining late-phase LTD. Cell Rep. 2021 Jul 20.(IF 9.420)浙江大學腦科學與腦醫(yī)學學院馬歡團隊

[15] Liu H, Xing R, et al. G-protein-coupled receptor GPR17 inhibits glioma development by increasing polycomb repressive complex 1-mediated ROS production. Cell Death Dis. 2021 Jun 12.(IF 8.463)廈門大學生命科學學院陳穎團隊

[16] Yan F, Huang C, et al. Threonine ADP-Ribosylation of Ubiquitin by a Bacterial Effector Family Blocks Host Ubiquitination. Mol Cell. 2020 May 21.(IF 17.97)浙江大學生命科學研究院朱永群團隊

[17] Luo Q, Wu X, et al. TRIM32/USP11 Balances ARID1A Stability and the Oncogenic/Tumor-Suppressive Status of Squamous Cell Carcinoma. Cell Rep. 2020 Jan 7.(IF 9.42)中國醫(yī)學科學院分子腫瘤學國家重點實驗室劉芝華團隊

[18] Sun X, Peng X, et al. ADNP promotes neural differentiation by modulating Wnt/β-catenin signaling. Nat Commun. 2020 Jun 12.(IF 14.911)中國科學院水生生物研究所孫玉華團隊

[19] Yang X, Wang H, et al. Rewiring ERBB3 and ERK signaling confers resistance to FGFR1 inhibition in gastrointestinal cancer harbored an ERBB3-E928G mutation. Protein Cell. 2020 Dec.(IF 14.872)浙江大學醫(yī)學院/轉化醫(yī)學研究院閔軍霞團隊

[20] Zou Y, Wang A, et al. Analysis of redox landscapes and dynamics in living cells and in vivo using genetically encoded fluorescent sensors. Nat Protoc. 2018 Oct.(IF 13.490)華東理工大學生物工程學院楊弋,、趙玉政團隊

[21] Zhang K, Zhao X, et al. Enhanced Therapeutic Effects of Mesenchymal Stem Cell-Derived Exosomes with an Injectable Hydrogel for Hindlimb Ischemia Treatment. ACS Appl Mater Interfaces. 2018 Sep 12.(IF 8.09)南開大學醫(yī)學院李宗金團隊

[22] Hao H, Hu S, et al. Loss of Endothelial CXCR7 Impairs Vascular Homeostasis and Cardiac Remodeling After Myocardial Infarction: Implications for Cardiovascular Drug Discovery. Circulation. 2017 Mar 28.(IF 29.69)中國醫(yī)學科學院/北京協(xié)和醫(yī)學院阜外醫(yī)院王淼團隊



PCR系列:


向下滑動查看


[1] Yu, Q., Liu, S., Yu, L. et al. RNA demethylation increases the yield and biomass of rice and potato plants in field trials. Nat Biotechnol (2021). (IF 54.908

[2] Dong W, Zhu Y, Chang H, et al. An SHR–SCR module specifies legume cortical cell fate to enable nodulation[J]. Nature, 2021, 589(7843): 586-590. (IF43

[3] Bi X, Wang K, Yang L.et al. Tracing the genetic footprints of vertebrate landing in non-teleost ray-finned fishes. Cell. (2021).  (IF 38.637)

[4] Liu S , Hua Y , Wang J , et al. RNA polymerase III is required for the repair of DNA double-strand breaks by homologous recombination[J]. Cell. (2021). (IF 38.637)

[5] Liu C , Li X. et.al. Structure and Degradation of Circular RNAs Regulate PKR Activation in Innate Immunity. Cell. (2019).  (IF 36.216)

[6] Lu, XY.,Shi, XJ., Hu, A.et al.Feeding induces cholesterol biosynthesis via the mTORC1–USP20–HMGCR axis.Nature(2020). (IF 43



科研路上高分paper發(fā)不停,小翌助您過關斬將,每天都出好數據,。


表1. 翌圣生物轉染試劑產品目錄
名稱
貨號
規(guī)格
價格(元)
Hieff TransTM Liposomal Transfection Reagent脂質體核酸轉染試劑
40802ES01
100 μL
168
40802ES02
0.5 mL
738
40802ES03
1.0 mL
1308
40802ES08
5×1mL
4878
Calcium Phosphate Cell Transfection Kit 磷酸鈣法細胞轉染試劑
40803ES70
200 T
625
Polybrene (hexadimethrine bromide) 聚凝胺(10 mg/ml)
40804ES76
500 μL
180
40804ES86
5×500 μL
500
Hieff TransTM Suspension Cell-Free Liposomal Transfection Reagent 懸浮細胞專用脂質體核酸轉染試劑
40805ES01
100 μL
228
40805ES02
0.5 mL
948
40805ES03
1.0 mL
1678
40805ES08
5×1 mL
5268
Hieff TransTM in vitro siRNA/miRNA Transfection Reagent siRNA/miRNA體外轉染試劑
40806ES01
0.1 mL
372
40806ES02
0.5 mL
1472
40806ES03
1.0 mL
2572
Polyethylenimine Linear(PEI) MW25000 線性PEI轉染試劑MW25000
40815ES03
1 g
1855
40815ES08
5×1 g
7255
Polyethylenimine Linear(PEI) MW40000(rapid lysis)線性PEI轉染試劑(速溶型)MW40000
40816ES02
100 mg
655
40816ES03
1 g
1855
40816ES08
5×1 g
7255


點擊產品名稱即可查看詳情

表2. 翌圣生物RT-qPCR產品目錄


方法
分類
產品名稱
貨號
qPCR染料法
高靈敏通用性qPCR預混液(染料法)
Hieff UNICON® Universal Blue qPCR SYBR® Master Mix(男神版)
11184ES
高特異型qPCR預混液(染料法)
Hieff UNICON® Power qPCR SYBR® Green Master Mix  (No Rox)
11195ES
Hieff UNICON® Power qPCR SYBR® Green Master Mix(Low Rox)
11196ES
Hieff UNICON® Power qPCR SYBR® Green Master Mix(High Rox)
11197ES
高靈敏型qPCR預混液(染料法)
Hieff UNICON® qPCR SYBR® Green Master Mix (No Rox)
11198ES
Hieff UNICON® qPCR SYBR® Green Master Mix (Low Rox)
11199ES
Hieff UNICON® qPCR SYBR® Green Master Mix (High Rox)
11200ES
普通型qPCR預混液(染料法)
Hieff® qPCR SYBR® Green Master Mix (No Rox )
11201ES
Hieff® qPCR SYBR® Green Master Mix (Low Rox )
11202ES
Hieff® qPCR SYBR® Green Master Mix (High Rox )
11203ES
miRNA qPCR加A法
Hieff® miRNA Universal  qPCR SYBR Master Mix(加A法)
11171ES
miRNA qPCR莖環(huán)法
Hieff® miRNA Universal  qPCR SYBR Master Mix(莖環(huán)法)
11170ES
RNA一步法RT-qPCR(染料法)
Hifair®  III One Step RT-qPCR SYBR Green Kit
11143ES
qPCR探針法
單重,、普通型qPCR預混液(探針法)
Hieff Unicon® qPCR TaqMan Probe Master Mix
11205ES
多重、高靈敏型qPCR預混液(探針法)
Universal TaqMan multiplex qPCR master mix
11211ES
RNA一步法RT-qPCR(探針法)
Hifair®  III One Step RT-qPCR Probe Kit
11145ES


點擊產品名稱即可查看詳情



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