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當(dāng)前位置：上海橋星貿(mào)易有限公司>>技術(shù)文章>>N-（3-二甲基氨基丙基）-N′-乙基碳二亞胺 鹽酸鹽
| InChI Key | FPQQSJJWHUJYPU-UHFFFAOYSA-N |
| product line | BioXtra |
| impurities | ≤0.005% Phosphorus (P) |
| ≤0.1% Insoluble matter | |
| ign. residue | ≤0.1% |
| mp | 110-115 °C (lit.) |
| solubility | H2O: 0.5 M, clear, colorless |
| anion traces | sulfate (SO42-): ≤0.05% |
| cation traces | Al: ≤0.0005% |
| Ca: ≤0.0005% | |
| Cu: ≤0.0005% | |
| Fe: ≤0.0005% | |
| K: ≤0.005% | |
| Mg: ≤0.0005% | |
| NH4+: ≤0.05% | |
| Na: ≤0.005% | |
| Pb: ≤0.001% | |
| Zn: ≤0.0005% | |
| storage temp. | −20°C |
包裝
1, 5, 10, 25 g in poly bottle
Application
N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride has been used:
• for post-fixation and crosslinking of small ribonucleic acid (RNAs) species from formalin fixed paraffin embedded (FFPE) tissues, for fluorescent in situ hybridisation (FISH) and immunofluorescence (IF) signals
• for cross-linking of nanofiber matrices with mouse lung extract
• for activation of substrate XNA oligonucleotides
Biochem/physiol Actions
水溶性縮合試劑,。EDAC通常用作與伯胺進(jìn)行酰胺鍵合的羧基活化劑,。另外，它能與磷酸基團(tuán)反應(yīng),。其已用于例如肽合成,、將蛋白質(zhì)與核酸交聯(lián)、以及免疫綴合物的制備,。通常,，EDAC在不含緩沖劑的pH范圍4.0-6.0的條件下使用。特別是,，應(yīng)避免使用胺和羧酸鹽緩沖液。
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