Merck蛋白抽提

MERCK蛋白抽提

1,、BugBuster®蛋白抽提試劑——尤其適用于GST融合蛋白的抽提

BugBuster蛋白抽提試劑用于溫和破壞大腸桿菌細(xì)胞壁以釋放活性蛋白,。作為激烈的機(jī)械法（如弗式壓榨法和超聲波法）的替代方法，這種試劑處理起來非常簡便,、快速,，且成本低廉，適合制備表達(dá)蛋白用于下游的純化等應(yīng)用,。

2,、BugBuster Master Mix預(yù)混蛋白抽提試劑

BugBuster Master Mix是將BugBuster蛋白抽提試劑，Benzonase核酸酶和rLysozyme™溶菌酶溶液按*化配比混合好的一種非常方便使用的蛋白抽提試劑,，有助于在*化條件下獲得可溶活性蛋白,。

Product	Size	Cat.No.	Price（￥）
BugBuster® Protein Extraction Reagent	100 ml	70584-3	750.00
BugBuster® Protein Extraction Reagent	500 ml	70584-4	3,789.00
BugBuster® Master Mix	100 ml	71456-3	1,700.00
BugBuster® Master Mix	500 ml	71456-4	5,200.00
CytoBuster™ Protein Extraction Reagent	50 ml	71009-3	824.00
CytoBuster™ Protein Extraction Reagent	250 ml	71009-4	3,122.00

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Comparison of E. coli lysis methods

50 ml samples of an induced 500 ml culture of BL21（DE3） containing pET-41a（+） encoding GST were harvested by centrifugation and resuspended in 2 ml 1X PBS, another commercially available protein extraction reagent, or BugBuster Reagent. The sample in PBS was sonicated with 10 pulses at 50% duty for 30 s total. Samples in lysis reagent were treated according to their respective protocols. Extracts were clarified by centrifugation and assayed for GST enzymatic activity.

3、Benzonase® 核酸酶——賽多利斯病毒純化試劑盒推薦使用

應(yīng)用范圍

² 蛋白抽提操作中降低樣品粘度
² 雙向電泳中樣品制備過程
² 重組蛋白樣品制備中去除核酸污染

Product	Size	Cat.No.	Price（￥）
Benzonase® Nuclease, Purity > 90%	10 ku	70746-3	1,200.00
Benzonase® Nuclease, Purity > 90%	2.5 ku	70746-4	400.00

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Viscosity reduction by Benzonase Nuclease

E. coli BL21（DE3） cells containing a pET construct were suspended in BugBuster Reagent （5 ml/g wet weight）. Aliquots of the suspension were treated with the indicated amounts of Benzonase Nuclease for 10 min at

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