| M-MLV Reverse Transcriptase M-MLV Reverse Transcriptase · M-MLV Reverse Transcriptase · M-MLV RT 5X Buffer | 10,000u | 200u/μl | M1701 |
QC Tests
Activity, SDS-PAGE/purity, DNase, RNase, endonuclease, first-strand cDNA synthesis.
Source
Recombinant E. coli strain.
Storage Buffer
20mM Tris-HCl （pH 7.5 at 25°C）, 0.2M NaCl, 0.1mM EDTA, 1mM DTT, 0.01% Nonidet® P-40 and 50% glycerol.
Storage Conditions
Store at –20°C.
Unit Definition
One unit is the amount of enzyme required to incorporate 1nmol of deoxynucleotide into acid-precipitable material in 10 minutes at 37°C. The reaction conditions are: 50mM Tris-HCl （pH 8.3 at 25°C）, 7mM MgCl2, 40mM KCl, 10mM DTT, 0.1mg/ml BSA, 0.5mM [3H]dTTP, 0.025mM oligo（dT）, 0.25mM poly（A） and 0.01% NP-40.
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