Capricorn HP-A說明書

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Capricorn Scientific成立于2013年,，是一家新的德國細(xì)胞培養(yǎng)基,，血清和試劑生產(chǎn)商。我們生產(chǎn)的主要重點(diǎn)是生產(chǎn)用于生物技術(shù),，診斷和研究應(yīng)用的高質(zhì)量動(dòng)物血清,。嚴(yán)格的測(cè)試程序以及全面的可追溯性記錄和文檔保證了我們產(chǎn)品的和可重復(fù)的質(zhì)量。

作為一家規(guī)模較小的公司,，我們可以靈活地提供各種傳統(tǒng)和定制產(chǎn)品,，以更低的成本，更短的交付周期和個(gè)性化的客戶服務(wù)滿足當(dāng)今市場(chǎng)不斷增長的需求,。

我們的技術(shù),，經(jīng)驗(yàn)豐富的專家團(tuán)隊(duì)多年來一直致力于細(xì)胞培養(yǎng)領(lǐng)域。我們致力于為活躍的細(xì)胞培養(yǎng)公司提供高質(zhì)量,，可靠性和服務(wù),。

Capricorn HP-A說明書

HybridomaPrime Serum-free Medium for Hybridoma Cells, with L-Glutamine, sterile-filtered

雜交瘤素?zé)o血清雜交瘤細(xì)胞培養(yǎng)基，含L-谷氨酰胺,，無菌過濾

貓編號(hào)：HP-A(500毫升)

產(chǎn)品說明

雜交瘤素是一種在無血清條件下培養(yǎng)氫化多巴細(xì)胞的固定培養(yǎng)基,。該培養(yǎng)基是一種單克隆抗體產(chǎn)生培養(yǎng)基，盡管它也被用來培養(yǎng)一種. 雜交瘤細(xì)胞系的多樣性。

商品概述

pH 7.0 – 7.5

內(nèi)毒素≤1 EU/ml

細(xì)胞培養(yǎng)試驗(yàn)S

柔軟性試驗(yàn)

儲(chǔ)存2°C至8°C

特征( feature的名詞復(fù)數(shù) )

-含有4毫米L-谷氨酰胺

-含有檸檬酸鐵

-不含動(dòng)物成分

-含有酵母抽提物,，使培養(yǎng)基達(dá)到良效果,。

重要信息

-培養(yǎng)基不含胰島素或轉(zhuǎn)鐵蛋白。補(bǔ)充胰島素(如5毫克/升胰島素)可進(jìn)一步改善這種培養(yǎng)基的性能,。

-雜交瘤素需要補(bǔ)充膽固醇補(bǔ)充劑或其他來源的膽固醇,，以生長依賴膽固醇的細(xì)胞系(如NS0和衍生物)。

-這種介質(zhì)不含表面活性劑,。如果用于攪拌懸浮培養(yǎng),，補(bǔ)充1g/l Pluronic F-68。

-在大多數(shù)情況下,，抗生素是不必要的,，也不是建議的,。然而，在需要抗生素的地方，大多數(shù)普通抗生素都與雜交瘤素兼容,，包括青霉素/鏈霉菌切開術(shù),。 CIN,、慶大霉素,、抗PPLO、亞諾辛和兩性霉素B不使用硫酸卡那霉素或新霉素硫酸鹽,。

培養(yǎng)條件

培養(yǎng)容器：搖瓶,、滾筒瓶或生物反應(yīng)器。

溫度范圍：36℃至38℃

孵化器氣氛：空氣中5%至10%CO2的加濕空氣,。確保適當(dāng)?shù)臍怏w交換,，盡量減少文化暴露在光線下。

細(xì)胞恢復(fù)

在37°C水浴中快速解凍(<1分鐘)冷凍細(xì)胞,。

將冷凍瓶的全部內(nèi)容轉(zhuǎn)移到不含抗生素的含30毫升預(yù)溫雜交瘤素的組織培養(yǎng)瓶中,。

37°C在空氣中5%CO2的加濕環(huán)境中孵育。

解凍后3~5天傳代細(xì)胞,。

使雜交瘤細(xì)胞適應(yīng)雜交瘤

成功的適應(yīng)將取決于特定的雜交瘤細(xì)胞株和所使用的培養(yǎng)條件,。我們建議將原始培養(yǎng)基中的備份培養(yǎng)保持到成功為止。新的媒介已經(jīng)實(shí)現(xiàn),。

注：細(xì)胞存活率至少為90%,，細(xì)胞在適應(yīng)前處于對(duì)數(shù)中期的生長階段是至關(guān)重要的。

直接適應(yīng),，直接適應(yīng)作用

傳代雜交瘤細(xì)胞在常規(guī)培養(yǎng)基中加入5%~10%血清或其他無血清培養(yǎng)基培養(yǎng)成預(yù)溫雜交瘤細(xì)胞,。在適應(yīng)過程中，播種密度應(yīng)加倍,。細(xì)胞系的正常播種密度,。

監(jiān)測(cè)細(xì)胞生長,，直到活細(xì)胞密度達(dá)到1×106個(gè)活細(xì)胞/ml。將細(xì)胞傳代至1~2×105個(gè)活細(xì)胞密度為1~2×105個(gè)/ml的新鮮預(yù)熱雜交瘤細(xì)胞中,。

繼續(xù)監(jiān)測(cè)和傳代細(xì)胞3至5代,，直到達(dá)到一致的生長。

注：如果使用直接適應(yīng)法在3~5通道上觀察到次優(yōu)性能,，則使用順序適應(yīng)法,。

順序適應(yīng)

傳代雜交瘤細(xì)胞在常規(guī)培養(yǎng)基中加入5%~10%的血清或其他無血清培養(yǎng)基培養(yǎng)成25：75的新鮮雜交瘤細(xì)胞與原培養(yǎng)基的比例。在適應(yīng)過程中r 種子密度為正常播種密度的兩倍,。

監(jiān)測(cè)細(xì)胞生長，直到活細(xì)胞密度達(dá)到1×106個(gè)活細(xì)胞/ml,。繼代培養(yǎng)細(xì)胞(稀釋至1-2×105個(gè)活細(xì)胞/ml),，使新鮮雜交素與或繼代培養(yǎng)基(50：50，75：25,，90：10,，其次是100雜交)。每個(gè)步驟可能需要多個(gè)通道,。

3.繼續(xù)監(jiān)測(cè)和傳代細(xì)胞,，直到細(xì)胞持續(xù)生長。在*雜交中進(jìn)行了幾次連續(xù)生長和存活的傳代后,，這種培養(yǎng)被認(rèn)為是適合的.

細(xì)胞冷凍保存

在組織培養(yǎng)瓶中準(zhǔn)備所需數(shù)量的細(xì)胞,，在生長中期收獲，活率>90%,。保留條件培養(yǎng)基準(zhǔn)備冷凍保存培養(yǎng)基,。

測(cè)定活細(xì)胞密度，計(jì)算冷凍保存培養(yǎng)基所需體積,，得出終細(xì)胞密度為0.5~1×107個(gè)/ml,。

在預(yù)定使用日制備92.5%培養(yǎng)基(新鮮培養(yǎng)基與條件培養(yǎng)基的比例為50：50)7.5%DMSO的要求體積。過濾,，消毒,，4°C保存，直到使用 ,。重要的是：條件培養(yǎng)基應(yīng)從高活力的中對(duì)數(shù)培養(yǎng)細(xì)胞中獲得.

100×g離心收集細(xì)胞5~10 min,。將球團(tuán)置于預(yù)先確定的4°C冷凍保存介質(zhì)中。

根據(jù)制造商的規(guī)格將此懸浮液配發(fā)到冷凍瓶中(即2毫升冰瓶中的1毫升),。

按照標(biāo)準(zhǔn)程序(每分鐘減少1°C),，在自動(dòng)化或手動(dòng)控制的冷凍設(shè)備中實(shí)現(xiàn)低溫保存。

將冷凍細(xì)胞轉(zhuǎn)移到液氮(蒸汽相),，建議儲(chǔ)存在-200°C至-125°C,。

預(yù)防措施及免責(zé)聲明

本產(chǎn)品僅供研究用,。請(qǐng)查閱材料安全數(shù)據(jù)表，以獲取有關(guān)危險(xiǎn)和安全處理做法的信息,。

Pluronic是巴斯夫的注冊(cè)商標(biāo),。

胎兒牛血清和其他血清

我們生產(chǎn)和測(cè)試了我們的生物加工和細(xì)胞培養(yǎng)血清產(chǎn)品，用于不同的哺乳動(dòng)物細(xì)胞培養(yǎng)應(yīng)用,。我們提供各種各樣的特色血清：

胎牛血清（FBS）
其他牛血清
其他動(dòng)物血清
人類血清

細(xì)胞培養(yǎng)基

液體培養(yǎng)基為所有細(xì)胞培養(yǎng)應(yīng)用提供基本營養(yǎng),。我們的每種高質(zhì)量細(xì)胞培養(yǎng)基都是根據(jù)初公布的配方或?qū)ε囵B(yǎng)基的一致性能和穩(wěn)定性*的修飾而制造的。使用摩羯座培養(yǎng)基是保證細(xì)胞成功生長的宜方法,。
我們提供各種產(chǎn)品：

經(jīng)典媒體
特別媒體
粉末介質(zhì)

試劑和補(bǔ)品

來自摩羯座的試劑和補(bǔ)充劑允許您定制細(xì)胞培養(yǎng)系統(tǒng)的生長條件,。所有產(chǎn)品都經(jīng)過嚴(yán)格測(cè)試和設(shè)計(jì)，以提供可重復(fù)性和良性能,。
我們提供各種各樣的產(chǎn)品：

牛血清白蛋白
拾遺
分離
抗生素
細(xì)胞分離
冷凍保存
去除支原體
轉(zhuǎn)染
細(xì)胞遺傳學(xué)
緩沖鹽解決方案

Capricorn Scientific產(chǎn)品目錄：

貨號(hào)	品名	規(guī)格	價(jià)格
DMEM-LPA	DMEM Low Glucose (1 g/l), with L-Glutamine, with Sodium Pyruvate	500 ml	7.50 €
DMEM-LPSTA	DMEM Low Glucose (1 g/l), with Stable Glutamine, with Sodium Pyruvate	500 ml	9.50 €
DMEM-LPXA	DMEM Low Glucose (1 g/l), w/o L-Glutamine, with Sodium Pyruvate	500 ml	7.50 €
DMEM-HA	DMEM High Glucose (4.5 g/l), with L-Glutamine	500 ml	6.40 €
DMEM-HHSTA	DMEM High Glucose (4.5 g/l), with Stable Glutamine, with 25 mM HEPES	500 ml	9.50 €
DMEM-HPA	DMEM High Glucose (4.5 g/l), with L-Glutamine, with Sodium Pyruvate	500 ml	7.50 €
DMEM-HPSTA	DMEM High Glucose (4.5 g/l), with Stable Glutamine, with Sodium Pyruvate	500 ml	7.00 €
DMEM-HPXA	DMEM High Glucose (4.5 g/l), w/o L-Glutamine, with Sodium Pyruvate	500 ml	7.50 €
DMEM-HXA	DMEM High Glucose (4.5 g/l), w/o L-Glutamine	500 ml	6.40 €
DMEM-HXRXA	DMEM High Glucose (4.5 g/l), w/o L-Glutamine, w/o Phenol Red	500 ml	10.50 €
DMEM-12-A	DMEM/Ham’s F-12, with L-Glutamine	500 ml	8.60 €
DMEM-12-HXA	DMEM/Ham’s F-12, w/o L-Glutamine, with 15 mM HEPES	500 ml	8.60 €
HAM-12-A	Ham’s F-12, with L-Glutamine	500 ml	9.50 €
IMDM-A	IMDM, with L-Glutamine, without Supplements	500 ml	9.00 €
L15-XA	Leibovitz’s L-15 Medium, w/o L-Glutamine	500 ml	12.40 €
MCC-A	McCoy’s Medium, with L-Glutamine	500 ml	12.70 €
M199-A	Medium 199, with L-Glutamine	500 ml	8.90 €
MEM-A	MEM with Earle’s Salts, with L-Glutamine	500 ml	7.20 €
MEM-STA	MEM with Earle’s Salts, with Stable Glutamine	500 ml	9.40 €
MEM-XA	MEM with Earle’s Salts, w/o L-Glutamine	500 ml	6.40 €
MEMA-RXA	MEM Alpha Modification, w/o L-Glutamine, with Nucleosides	500 ml	19.90 €
MEMA-XRXA	MEM Alpha Modification, w/o L-Glutamine, w/o Nucleosides	500 ml	13.00 €
RPMI-A	RPMI 1640, with L-Glutamine	500 ml	6.40 €
RPMI-HA	RPMI 1640, with L-Glutamine, with 25 mM HEPES	500 ml	9.90 €
RPMI-HXA	RPMI 1640, w/o L-Glutamine, with 25 mM HEPES	500 ml	9.50 €
RPMI-STA	RPMI 1640, with Stable Glutamine	500 ml	8.40 €
RPMI-XA	RPMI 1640, w/o L-Glutamine	500 ml	5.80 €
RPMI-XRXA	RPMI 1640, w/o L-Glutamine, w/o Phenol Red	500 ml	9.90 €
FM1-F	FreezeMe One, Cryopreservation Medium with Fetal Bovine Serum	50 ml	65.00 €
FM2-F	FreezeMe Two, Cryopreservation Medium Serum-free	50 ml	70.00 €
TP-A	TumorPlus 263, Complete Medium for Tumor Cells, with L-Glutamine	500 ml	29.90 €
HP-A	HybridomaPrime, Hybridoma Medium, Serum-free	500 ml	34.90 €
HY-A	HybridomaPlus, Hybridoma Medium, Protein-free	500 ml	39.90 €
FP-A	FibroPlus 333 Complete Medium for Fibroblasts, with L-Glutamine	500 ml	29.90 €
ERP-B	EndoPrime Complete Medium for C*tion of Primary Cells	100 ml	on request
ERP-A	EndoPrime Complete Medium for C*tion of Primary Cells	500 ml	on request
HPR-B	HepaPrime Complete Medium for C*tion of Primary Hepatocytes	100 ml	on request
HPR-A	HepaPrime Complete Medium for C*tion of Primary Hepatocytes	500 ml	on request
NKR-G	NK-Prime Complete Medium for C*tion of Primary Natural Killer Cells	20 ml	on request
NKR-B	NK-Prime Complete Medium for C*tion of Primary Natural Killer Cells	100 ml	on request
CHOA-500ML	CHO-ONE A	500 ml	45.00 €
CHOE-500ML	CHO-ONE E	500 ml	49.00 €
CHOE-1000ML	CHO-ONE E	1000ml	89.00 €
CHOF1-100ML	CHO-ONE F1	100 ml	23.00 €
CHOF1-500ML	CHO-ONE F1	500 ml	95.00 €
CHOF2-10ML	CHO-ONE F2	10 ml	15.00 €
CHOF2-50ML	CHO-ONE F2	50 ml	49.00 €
CHO-K1	CHO-ONE Starter Kit		169.00 €
CHO-K2	CHO-ONE Expansion Kit		950.00 €
DMEM-LPA-P10	DMEM Low Glucose (1 g/l) powder medium, with L-Glutamine, with Sodium Pyruvate, w/o Sodium Bicarbonate	10 L	14.90 €
DMEM-LPA-P50	DMEM Low Glucose (1 g/l) powder medium, with L-Glutamine, with Sodium Pyruvate, w/o Sodium Bicarbonate	50 L	62.90 €
DMEM-HA-P10	DMEM High Glucose (4.5 g/l) powder medium, with L-Glutamine, w/o Sodium Bicarbonate	10 L	15.50 €
DMEM-HA-P50	DMEM High Glucose (4.5 g/l) powder medium, with L-Glutamine, w/o Sodium Bicarbonate	50 L	66.50 €
DMEM-HPA-P10	DMEM High Glucose (4.5 g/l) powder medium, with L-Glutamine, with Sodium Pyruvate, w/o Sodium Bicarbonate	10 L	15.50 €
DMEM-HPA-P50	DMEM High Glucose (4.5 g/l) powder medium, with L-Glutamine, with Sodium Pyruvate, w/o Sodium Bicarbonate	50 L	66.50 €
MEM-A-P10	MEM with Earle’s Salts powder medium, with L-Glutamine, w/o Sodium Bicarbonate	10 L	14.90 €
MEM-A-P50	MEM with Earle’s Salts powder medium, with L-Glutamine, w/o Sodium Bicarbonate	50 L	62.90 €
RPMI-A-P10	RPMI 1640 powder medium, with L-Glutamine, w/o Sodium Bicarbonate	10 L	15.50 €
RPMI-A-P50	RPMI 1640 powder medium, with L-Glutamine, w/o Sodium Bicarbonate	50 L	66.50 €
RPMI-HA-P10	RPMI 1640 powder medium, with L-Glutamine, with 25 mM HEPES, w/o Sodium Bicarbonate	10 L	20.10 €
RPMI-HA-P50	RPMI 1640 powder medium, with L-Glutamine, with 25 mM HEPES, w/o Sodium Bicarbonate	50 L	89.90 €
FBS-52B	Fetal Bovine Serum, Collected in Australia	100 ml	on request
FBS-52A	Fetal Bovine Serum, Collected in Australia	500 ml	on request
FBS-12B	Fetal Bovine Serum, Collected in South America	100 ml	on request
FBS-12A	Fetal Bovine Serum, Collected in South America	500 ml	on request
FBS-22B	Fetal Bovine Serum, Collected in USA	100 ml	on request
FBS-22A	Fetal Bovine Serum, Collected in USA	500 ml	on request
FBS-HI-12B	Fetal Bovine Serum, Heat Inactivated, Collected in South America	100 ml	on request
FBS-HI-12A	Fetal Bovine Serum, Heat Inactivated, Collected in South America	500 ml	on request
FBS-HI-22B	Fetal Bovine Serum, Heat Inactivated, Collected in USA	100 ml	on request
FBS-HI-22A	Fetal Bovine Serum, Heat Inactivated, Collected in USA	500 ml	on request
FBS-11B	Fetal Bovine Serum Advanced, Collected in South America	100 ml	on request
FBS-11A	Fetal Bovine Serum Advanced, Collected in South America	500 ml	on request
FBS-HI-11B	Fetal Bovine Serum Advanced, Heat Inactivated, Collected in South America	100 ml	on request
FBS-HI-11A	Fetal Bovine Serum Advanced, Heat Inactivated, Collected in South America	500 ml	on request
FBS-TET-12B	Fetal Bovine Serum, Tetracycline Negative, Collected in South America	100 ml	on request
FBS-TET-12A	Fetal Bovine Serum, Tetracycline Negative, Collected in South America	500 ml	on request
FBS-ES-22B	Fetal Bovine Serum, ES cell pre-tested, Collected in USA	100 ml	on request
FBS-ES-22A	Fetal Bovine Serum, ES cell pre-tested, Collected in USA	500 ml	on request
FBS-DIA-12B	Fetal Bovine Serum, Dialysed, Collected in South America	100 ml	on request
FBS-DIA-12A	Fetal Bovine Serum, Dialysed, Collected in South America	500 ml	on request
FBS-IG-12B	Fetal Bovine Serum, IgG Stripped, Collected in South America	100 ml	on request
FBS-IG-12B	Fetal Bovine Serum, IgG Stripped, Collected in South America	500 ml	on request
FBS-ED-12F	Fetal Bovine Serum Exosome Depleted	50 ml	on request
FBS-ED-12B	Fetal Bovine Serum Exosome Depleted	100 ml	on request
FBS-ED-12A	Fetal Bovine Serum Exosome Depleted	500 ml	on request
NCS-1B	Newborn Calf Serum	100 ml	12.00 €
NCS-1A	Newborn Calf Serum	500 ml	38.00 €
NCS-HI-1B	Newborn Calf Serum, Heat Inactivated	100 ml	13.10 €
NCS-HI-1A	Newborn Calf Serum, Heat Inactivated	500 ml	43.00 €
CS-1B	Calf Serum	100 ml	8.00 €
CS-1A	Calf Serum	500 ml	29.50 €
ABS-1B	Adult Bovine Serum	100 ml	8.00 €
ABS-1A	Adult Bovine Serum	500 ml	29.50 €
ABS-1C	Adult Bovine Serum	1000 ml	on reques
HOS-1B	Horse Serum	100 ml	7.20 €
HOS-1A	Horse Serum	500 ml	32.00 €
DHS-1B	Donor Horse Serum	100 ml	9.00 €
DHS-1A	Donor Horse Serum	500 ml	35.00 €
GOA-1B	Goat Serum	100 ml	24.00 €
GOA-1A	Goat Serum	500 ml	105.00 €
POR-1B	Porcine Serum	100 ml	10.50 €
POR-1A	Porcine Serum	500 ml	39.00 €
MOU-1B	Mouse Serum	100 ml	499.00 €
RAT-1B	Rat Serum	100 ml	499.00 €
RAB-1B	Rabbit Serum	100 ml	99.00 €
HUM-3B	Human Serum, Type AB, Male	100 ml	134.00 €
BSA-1U	Bovine Serum Albumin, Fraction V, pH 7.0, Origin USA	50 g	56.00 €
BSA-1S	Bovine Serum Albumin, Fraction V, pH 7.0, Origin USA	100 g	77.00 €
BSA-1V	Bovine Serum Albumin, Fraction V, pH 7.0, Origin USA	250 g	on request
BSA-1T	Bovine Serum Albumin, Fraction V, pH 7.0, Origin USA	500 g	299.00 €
BSA-FAF-1U	Bovine Serum Albumin, Fatty Acid Free, Origin USA	50 g	99.00 €
BSA-FAF-1S	Bovine Serum Albumin, Fatty Acid Free, Origin USA	100 g	178.00 €
BSA-PF-1U	Bovine Serum Albumin, Protease Free, Origin USA	50 g	88.00 €
BSA-PF-1S	Bovine Serum Albumin, Protease Free, Origin USA	100 g	151.00 €
HSA-1R	Human Serum Albumin	10 g	56.00 €
HSA-1U	Human Serum Albumin	50 g	227.00 €
HSA-1S	Human Serum Albumin	100 g	420.00 €
N2-K	N2 Supplement (100x), Serum-free	5 ml	45.00 €
C21-H	NCS21 Supplement (50x), Serum-free	10 ml	45.00 €
ITS-H	ITS (100x), Insulin, Transferrin, Selenium	10 ml	25.00 €
HCS-F	Hybridoma Supplément, Serum-free	50 ml	99.00 €
GLN-B	L-Glutamine, 200 mM	100 ml	8.00 €
STA-B	Stable Glutamine, 200 mM	100 ml	14.50 €
NPY-B	Sodium Pyruvate, 100 mM	100 ml	10.50 €
NEAA-B	MEM Nonessential Amino Acids (100x)	100 ml	8.50 €
ACC-1B	Accutase Cell Detachment Solution	100 ml	26.00 €
ACM-1G	AccuMax Cell Detachment Solution	20 ml	29.00 €
ACM-1F	AccuMax Cell Detachment Solution	50 ml	69.00 €
TRY-1B	Trypsin-EDTA (0.05 %) in DPBS (1x)	100 ml	7.00 €
TRY-1B10	Trypsin-EDTA (0.5 %) in DPBS (10x)	100 ml	11.50 €
TRY-2B	Trypsin (0.25 %) in DPBS (1x)	100 ml	7.00 €
TRY-2B10	Trypsin (2.5 %) in DPBS (10x)	100 ml	11.50 €
TRY-3B	Trypsin-EDTA (0.25 %) in HBSS (1x) with Phenol Red	100 ml	9.50 €
TRY-4B	Trypsin-EDTA (0.05 %) in HBSS (1x) with Phenol Red	100 ml	8.50 €
AMP-B	Amphotericin B Solution (250 µg/ml)	100 ml	14.00 €
AAS-B	Antibiotic/Antimycotic Solution (100x)	100 ml	14.00 €
GEN-10B	Gentamicin Solution (10 mg/ml)	100 ml	50.00 €
PS-B	Penicillin/Streptomycin (100x)	100 ml	8.00 €
PSG-B	Penicillin/Streptomycin (100x), with L-Glutamine	100 ml	15.00 €
MYX-B	MycoXpert, Mycoplasma Removal Reagent (50x)	100 ml	99.00 €
LSM-B	Lymphocytes Separation Media, Density 1.077 g/ml	100 ml	12.00 €
LSM-A	Lymphocytes Separation Media, Density 1.077 g/ml	500 ml	36.50 €
FM1-F	FreezeMe One, Cryopreservation Medium with Fetal Bovine Serum	50 ml	65.00 €
FM2-F	FreezeMe Two, Cryopreservation Medium Serum-free	50 ml	70.00 €
MYX-B	MycoXpert, Mycoplasma Removal Reagent (50x)	100 ml	99.00 €
PBS-10XA	Dulbecco's PBS (10x), w/o Ca & Mg, w/o Phenol Red	500 ml	13.00 €
PBS-1A	Dulbecco's PBS (1x), w/o Ca & Mg, w/o Phenol Red	500 ml	6.50 €
PBS-2A	Dulbecco's PBS (1x), with Ca & Mg, w/o Phenol Red	500 ml	8.00 €
HBSS-1A	Hanks' Balanced Salts (1x), with Ca & Mg, w/o Phenol Red	500 ml	7.50 €
HBSS-2A	Hanks' Balanced Salts (1x), w/o Ca & Mg, w/o Phenol Red	500 ml	7.00 €
HBSS-3A	Hanks' Balanced Salts (1x), w/o Ca & Mg, with Phenol Red	500 ml	7.50 €
HEP-B	HEPES Buffer Solution (1 M)	100 ml	39.00 €
PBS-1A-P10	Dulbecco's PBS (1x) powder, w/o Ca & Mg, w/o Phenol Red	10 L	10.90 €
PBS-1A-P50	Dulbecco's PBS (1x) powder, w/o Ca & Mg, w/o Phenol Red	50 L	43.10 €

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6：我們還是Santa,Advanced Biotechnologies Inc,Athens Research & Technology,bangs,BBInternational,crystalchem,dianova,FD Neurotechnologies,Inc. FormuMax Scientific,Inc, Genebridege, Glycotope Biotechnology GmbH; iduron,Innovative Research of America, Ludger, neuroprobe,omicronbio, Polysciences,prospecbi, QA-BIO,quickzyme,RESEARCH DIETS,INC,sterlitech,；sysy,TriLink BioTechnologies,Inc,；worthington-biochem,zyagen等幾十家國外公司授權(quán)代理。

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