Capricorn FM1-F說明書

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Capricorn Scientific成立于2013年，是一家新的德國細(xì)胞培養(yǎng)基,，血清和試劑生產(chǎn)商,。我們生產(chǎn)的主要重點(diǎn)是生產(chǎn)用于生物技術(shù)，診斷和研究應(yīng)用的高質(zhì)量動物血清,。嚴(yán)格的測試程序以及全面的可追溯性記錄和文檔保證了我們產(chǎn)品的和可重復(fù)的質(zhì)量,。

作為一家規(guī)模較小的公司，我們可以靈活地提供各種傳統(tǒng)和定制產(chǎn)品,，以更低的成本,，更短的交付周期和個性化的客戶服務(wù)滿足當(dāng)今市場不斷增長的需求。

我們的技術(shù),，經(jīng)驗豐富的專家團(tuán)隊多年來一直致力于細(xì)胞培養(yǎng)領(lǐng)域,。我們致力于為活躍的細(xì)胞培養(yǎng)公司提供高質(zhì)量，可靠性和服務(wù),。

Capricorn FM1-F說明書

冷凍一種含胎牛血清的細(xì)胞保藏培養(yǎng)基

貓編號：FM1-F(50毫升)

產(chǎn)品闡述,，產(chǎn)品說明

凍干粉是一種含有10%DMSO和20%FBS的冷凍保護(hù)介質(zhì)。所用的血清在細(xì)胞保存期間被廣泛測試以保護(hù)細(xì)胞,。

申請

-冷凍保存多種高活力的細(xì)胞類型

-現(xiàn)成的解決方案

商品概述

不育試驗

貯存≤-15°C.凍干粉是一種光敏溶液.在運(yùn)輸和儲存期間,，應(yīng)該保護(hù)它不受光線的影響。

凍結(jié)議定書

在冷凍保存前,，應(yīng)檢查細(xì)胞是否受到污染,。FreezeMe可以與任何標(biāo)準(zhǔn)的冷凍協(xié)議一起使用。

懸浮培養(yǎng)物的低溫保存

-計算冷凍保存的活細(xì)胞數(shù),。細(xì)胞應(yīng)處于生長中期,。離心5 min至顆粒細(xì)胞(200~400 g)。把上清液移到小的在不打擾細(xì)胞的情況下,。

-預(yù)冷(4°C~8°C)的復(fù)蘇細(xì)胞(5x106~107細(xì)胞/ml),。

-進(jìn)入低溫儲存瓶。將小瓶置于4°C,，5 min內(nèi)開始冷凍,。細(xì)胞以1°C/min的速度緩慢冷凍(可編程冷卻器或?qū)⑿∑糠湃雿u葉) 在攝氏-70度至攝氏-90度的冷凍機(jī)內(nèi)放置箱)。

-然后將儲存瓶轉(zhuǎn)移到液氮儲存,。

貼壁培養(yǎng)的冷凍保存

-用溫和的解離劑將細(xì)胞與底物分離,。特別是敏感細(xì)胞使用酸性磷酸酶(CAT)。否避免細(xì)胞損傷,。必要時滅活解離劑,。

-在完整的培養(yǎng)基中復(fù)蘇分離的細(xì)胞，并建立可行的細(xì)胞計數(shù),。

離心5 min至顆粒細(xì)胞(200~400 g),。將上清液移至小體積,，而不干擾細(xì)胞。

-預(yù)冷(4°C~8°C)的復(fù)蘇細(xì)胞(5x106~107細(xì)胞/ml),。

-進(jìn)入低溫儲存瓶,。將小瓶置于4°C，5 min內(nèi)開始冷凍,。細(xì)胞以1°C/min的速度緩慢冷凍(可編程冷卻器或?qū)⑿∑糠湃雿u葉) 在攝氏-70度至攝氏-90度的冷凍機(jī)內(nèi)放置箱),。

-然后將儲存瓶轉(zhuǎn)移到液氮儲存。

凍存細(xì)胞可通過以下程序解凍

離心法,，離心過濾

-從儲存中取出細(xì)胞,，在37°C水浴中迅速解凍。摩羯座科學(xué)推薦使用經(jīng)批準(zhǔn)的安全護(hù)目鏡來保護(hù)眼睛,。我們還建議使用安全手套來保護(hù) 裸露的皮膚,。-將1至2毫升的解凍細(xì)胞放入25毫升的*培養(yǎng)基中。輕輕混合細(xì)胞懸液,。

-80 g離心2~3 min,。

-檢查細(xì)胞顆粒的上清和可見度。在不干擾細(xì)胞的情況下丟棄上清液,。

-輕輕地將細(xì)胞重新懸浮在完整的培養(yǎng)基中,，并進(jìn)行可行的細(xì)胞計數(shù)。

-打平細(xì)胞,。細(xì)胞接種量應(yīng)至少為3x105個活細(xì)胞/ml,。

直接電鍍

-從儲存中取出細(xì)胞，在37°C水浴中迅速解凍,。摩羯座科學(xué)推薦使用經(jīng)批準(zhǔn)的安全護(hù)目鏡來保護(hù)眼睛,。我們還建議使用安全手套來保護(hù) 裸露的皮膚,。

-板細(xì)胞直接與完整的生長培養(yǎng)基,。每1毫升冷凍細(xì)胞使用10至20毫升完整培養(yǎng)基。細(xì)胞接種量應(yīng)至少為3x105細(xì)胞/ml,。

-培養(yǎng)細(xì)胞12至24小時,，用新鮮的*培養(yǎng)基替代培養(yǎng)基，去除冷凍保存液,。

我們建議解凍程序1,，特別是當(dāng)處理敏感細(xì)胞。

預(yù)防措施及免責(zé)聲明

本產(chǎn)品僅供研究用,。請查閱材料安全數(shù)據(jù)表,，以獲取有關(guān)危險和安全處理做法的信息。

警告：冷凍Me含有二甲基亞砜(DMSO),。不要吸入氣體/煙霧/蒸汽/噴霧,。避免接觸眼睛和皮膚,。對眼睛、呼吸系統(tǒng)和皮膚有刺激性,。S-23 S-24/25,。

胎兒牛血清和其他血清

我們生產(chǎn)和測試了我們的生物加工和細(xì)胞培養(yǎng)血清產(chǎn)品，用于不同的哺乳動物細(xì)胞培養(yǎng)應(yīng)用,。我們提供各種各樣的特色血清：

胎牛血清（FBS）
其他牛血清
其他動物血清
人類血清

細(xì)胞培養(yǎng)基

液體培養(yǎng)基為所有細(xì)胞培養(yǎng)應(yīng)用提供基本營養(yǎng),。我們的每種高質(zhì)量細(xì)胞培養(yǎng)基都是根據(jù)初公布的配方或?qū)ε囵B(yǎng)基的一致性能和穩(wěn)定性*的修飾而制造的。使用摩羯座培養(yǎng)基是保證細(xì)胞成功生長的宜方法,。
我們提供各種產(chǎn)品：

經(jīng)典媒體
特別媒體
粉末介質(zhì)

試劑和補(bǔ)品

來自摩羯座的試劑和補(bǔ)充劑允許您定制細(xì)胞培養(yǎng)系統(tǒng)的生長條件,。所有產(chǎn)品都經(jīng)過嚴(yán)格測試和設(shè)計，以提供可重復(fù)性和良性能,。
我們提供各種各樣的產(chǎn)品：

牛血清白蛋白
拾遺
分離
抗生素
細(xì)胞分離
冷凍保存
去除支原體
轉(zhuǎn)染
細(xì)胞遺傳學(xué)
緩沖鹽解決方案

Capricorn Scientific產(chǎn)品目錄：

貨號	品名	規(guī)格	價格
DMEM-LPA	DMEM Low Glucose (1 g/l), with L-Glutamine, with Sodium Pyruvate	500 ml	7.50 €
DMEM-LPSTA	DMEM Low Glucose (1 g/l), with Stable Glutamine, with Sodium Pyruvate	500 ml	9.50 €
DMEM-LPXA	DMEM Low Glucose (1 g/l), w/o L-Glutamine, with Sodium Pyruvate	500 ml	7.50 €
DMEM-HA	DMEM High Glucose (4.5 g/l), with L-Glutamine	500 ml	6.40 €
DMEM-HHSTA	DMEM High Glucose (4.5 g/l), with Stable Glutamine, with 25 mM HEPES	500 ml	9.50 €
DMEM-HPA	DMEM High Glucose (4.5 g/l), with L-Glutamine, with Sodium Pyruvate	500 ml	7.50 €
DMEM-HPSTA	DMEM High Glucose (4.5 g/l), with Stable Glutamine, with Sodium Pyruvate	500 ml	7.00 €
DMEM-HPXA	DMEM High Glucose (4.5 g/l), w/o L-Glutamine, with Sodium Pyruvate	500 ml	7.50 €
DMEM-HXA	DMEM High Glucose (4.5 g/l), w/o L-Glutamine	500 ml	6.40 €
DMEM-HXRXA	DMEM High Glucose (4.5 g/l), w/o L-Glutamine, w/o Phenol Red	500 ml	10.50 €
DMEM-12-A	DMEM/Ham’s F-12, with L-Glutamine	500 ml	8.60 €
DMEM-12-HXA	DMEM/Ham’s F-12, w/o L-Glutamine, with 15 mM HEPES	500 ml	8.60 €
HAM-12-A	Ham’s F-12, with L-Glutamine	500 ml	9.50 €
IMDM-A	IMDM, with L-Glutamine, without Supplements	500 ml	9.00 €
L15-XA	Leibovitz’s L-15 Medium, w/o L-Glutamine	500 ml	12.40 €
MCC-A	McCoy’s Medium, with L-Glutamine	500 ml	12.70 €
M199-A	Medium 199, with L-Glutamine	500 ml	8.90 €
MEM-A	MEM with Earle’s Salts, with L-Glutamine	500 ml	7.20 €
MEM-STA	MEM with Earle’s Salts, with Stable Glutamine	500 ml	9.40 €
MEM-XA	MEM with Earle’s Salts, w/o L-Glutamine	500 ml	6.40 €
MEMA-RXA	MEM Alpha Modification, w/o L-Glutamine, with Nucleosides	500 ml	19.90 €
MEMA-XRXA	MEM Alpha Modification, w/o L-Glutamine, w/o Nucleosides	500 ml	13.00 €
RPMI-A	RPMI 1640, with L-Glutamine	500 ml	6.40 €
RPMI-HA	RPMI 1640, with L-Glutamine, with 25 mM HEPES	500 ml	9.90 €
RPMI-HXA	RPMI 1640, w/o L-Glutamine, with 25 mM HEPES	500 ml	9.50 €
RPMI-STA	RPMI 1640, with Stable Glutamine	500 ml	8.40 €
RPMI-XA	RPMI 1640, w/o L-Glutamine	500 ml	5.80 €
RPMI-XRXA	RPMI 1640, w/o L-Glutamine, w/o Phenol Red	500 ml	9.90 €
FM1-F	FreezeMe One, Cryopreservation Medium with Fetal Bovine Serum	50 ml	65.00 €
FM2-F	FreezeMe Two, Cryopreservation Medium Serum-free	50 ml	70.00 €
TP-A	TumorPlus 263, Complete Medium for Tumor Cells, with L-Glutamine	500 ml	29.90 €
HP-A	HybridomaPrime, Hybridoma Medium, Serum-free	500 ml	34.90 €
HY-A	HybridomaPlus, Hybridoma Medium, Protein-free	500 ml	39.90 €
FP-A	FibroPlus 333 Complete Medium for Fibroblasts, with L-Glutamine	500 ml	29.90 €
ERP-B	EndoPrime Complete Medium for C*tion of Primary Cells	100 ml	on request
ERP-A	EndoPrime Complete Medium for C*tion of Primary Cells	500 ml	on request
HPR-B	HepaPrime Complete Medium for C*tion of Primary Hepatocytes	100 ml	on request
HPR-A	HepaPrime Complete Medium for C*tion of Primary Hepatocytes	500 ml	on request
NKR-G	NK-Prime Complete Medium for C*tion of Primary Natural Killer Cells	20 ml	on request
NKR-B	NK-Prime Complete Medium for C*tion of Primary Natural Killer Cells	100 ml	on request
CHOA-500ML	CHO-ONE A	500 ml	45.00 €
CHOE-500ML	CHO-ONE E	500 ml	49.00 €
CHOE-1000ML	CHO-ONE E	1000ml	89.00 €
CHOF1-100ML	CHO-ONE F1	100 ml	23.00 €
CHOF1-500ML	CHO-ONE F1	500 ml	95.00 €
CHOF2-10ML	CHO-ONE F2	10 ml	15.00 €
CHOF2-50ML	CHO-ONE F2	50 ml	49.00 €
CHO-K1	CHO-ONE Starter Kit		169.00 €
CHO-K2	CHO-ONE Expansion Kit		950.00 €
DMEM-LPA-P10	DMEM Low Glucose (1 g/l) powder medium, with L-Glutamine, with Sodium Pyruvate, w/o Sodium Bicarbonate	10 L	14.90 €
DMEM-LPA-P50	DMEM Low Glucose (1 g/l) powder medium, with L-Glutamine, with Sodium Pyruvate, w/o Sodium Bicarbonate	50 L	62.90 €
DMEM-HA-P10	DMEM High Glucose (4.5 g/l) powder medium, with L-Glutamine, w/o Sodium Bicarbonate	10 L	15.50 €
DMEM-HA-P50	DMEM High Glucose (4.5 g/l) powder medium, with L-Glutamine, w/o Sodium Bicarbonate	50 L	66.50 €
DMEM-HPA-P10	DMEM High Glucose (4.5 g/l) powder medium, with L-Glutamine, with Sodium Pyruvate, w/o Sodium Bicarbonate	10 L	15.50 €
DMEM-HPA-P50	DMEM High Glucose (4.5 g/l) powder medium, with L-Glutamine, with Sodium Pyruvate, w/o Sodium Bicarbonate	50 L	66.50 €
MEM-A-P10	MEM with Earle’s Salts powder medium, with L-Glutamine, w/o Sodium Bicarbonate	10 L	14.90 €
MEM-A-P50	MEM with Earle’s Salts powder medium, with L-Glutamine, w/o Sodium Bicarbonate	50 L	62.90 €
RPMI-A-P10	RPMI 1640 powder medium, with L-Glutamine, w/o Sodium Bicarbonate	10 L	15.50 €
RPMI-A-P50	RPMI 1640 powder medium, with L-Glutamine, w/o Sodium Bicarbonate	50 L	66.50 €
RPMI-HA-P10	RPMI 1640 powder medium, with L-Glutamine, with 25 mM HEPES, w/o Sodium Bicarbonate	10 L	20.10 €
RPMI-HA-P50	RPMI 1640 powder medium, with L-Glutamine, with 25 mM HEPES, w/o Sodium Bicarbonate	50 L	89.90 €
FBS-52B	Fetal Bovine Serum, Collected in Australia	100 ml	on request
FBS-52A	Fetal Bovine Serum, Collected in Australia	500 ml	on request
FBS-12B	Fetal Bovine Serum, Collected in South America	100 ml	on request
FBS-12A	Fetal Bovine Serum, Collected in South America	500 ml	on request
FBS-22B	Fetal Bovine Serum, Collected in USA	100 ml	on request
FBS-22A	Fetal Bovine Serum, Collected in USA	500 ml	on request
FBS-HI-12B	Fetal Bovine Serum, Heat Inactivated, Collected in South America	100 ml	on request
FBS-HI-12A	Fetal Bovine Serum, Heat Inactivated, Collected in South America	500 ml	on request
FBS-HI-22B	Fetal Bovine Serum, Heat Inactivated, Collected in USA	100 ml	on request
FBS-HI-22A	Fetal Bovine Serum, Heat Inactivated, Collected in USA	500 ml	on request
FBS-11B	Fetal Bovine Serum Advanced, Collected in South America	100 ml	on request
FBS-11A	Fetal Bovine Serum Advanced, Collected in South America	500 ml	on request
FBS-HI-11B	Fetal Bovine Serum Advanced, Heat Inactivated, Collected in South America	100 ml	on request
FBS-HI-11A	Fetal Bovine Serum Advanced, Heat Inactivated, Collected in South America	500 ml	on request
FBS-TET-12B	Fetal Bovine Serum, Tetracycline Negative, Collected in South America	100 ml	on request
FBS-TET-12A	Fetal Bovine Serum, Tetracycline Negative, Collected in South America	500 ml	on request
FBS-ES-22B	Fetal Bovine Serum, ES cell pre-tested, Collected in USA	100 ml	on request
FBS-ES-22A	Fetal Bovine Serum, ES cell pre-tested, Collected in USA	500 ml	on request
FBS-DIA-12B	Fetal Bovine Serum, Dialysed, Collected in South America	100 ml	on request
FBS-DIA-12A	Fetal Bovine Serum, Dialysed, Collected in South America	500 ml	on request
FBS-IG-12B	Fetal Bovine Serum, IgG Stripped, Collected in South America	100 ml	on request
FBS-IG-12B	Fetal Bovine Serum, IgG Stripped, Collected in South America	500 ml	on request
FBS-ED-12F	Fetal Bovine Serum Exosome Depleted	50 ml	on request
FBS-ED-12B	Fetal Bovine Serum Exosome Depleted	100 ml	on request
FBS-ED-12A	Fetal Bovine Serum Exosome Depleted	500 ml	on request
NCS-1B	Newborn Calf Serum	100 ml	12.00 €
NCS-1A	Newborn Calf Serum	500 ml	38.00 €
NCS-HI-1B	Newborn Calf Serum, Heat Inactivated	100 ml	13.10 €
NCS-HI-1A	Newborn Calf Serum, Heat Inactivated	500 ml	43.00 €
CS-1B	Calf Serum	100 ml	8.00 €
CS-1A	Calf Serum	500 ml	29.50 €
ABS-1B	Adult Bovine Serum	100 ml	8.00 €
ABS-1A	Adult Bovine Serum	500 ml	29.50 €
ABS-1C	Adult Bovine Serum	1000 ml	on reques
HOS-1B	Horse Serum	100 ml	7.20 €
HOS-1A	Horse Serum	500 ml	32.00 €
DHS-1B	Donor Horse Serum	100 ml	9.00 €
DHS-1A	Donor Horse Serum	500 ml	35.00 €
GOA-1B	Goat Serum	100 ml	24.00 €
GOA-1A	Goat Serum	500 ml	105.00 €
POR-1B	Porcine Serum	100 ml	10.50 €
POR-1A	Porcine Serum	500 ml	39.00 €
MOU-1B	Mouse Serum	100 ml	499.00 €
RAT-1B	Rat Serum	100 ml	499.00 €
RAB-1B	Rabbit Serum	100 ml	99.00 €
HUM-3B	Human Serum, Type AB, Male	100 ml	134.00 €
BSA-1U	Bovine Serum Albumin, Fraction V, pH 7.0, Origin USA	50 g	56.00 €
BSA-1S	Bovine Serum Albumin, Fraction V, pH 7.0, Origin USA	100 g	77.00 €
BSA-1V	Bovine Serum Albumin, Fraction V, pH 7.0, Origin USA	250 g	on request
BSA-1T	Bovine Serum Albumin, Fraction V, pH 7.0, Origin USA	500 g	299.00 €
BSA-FAF-1U	Bovine Serum Albumin, Fatty Acid Free, Origin USA	50 g	99.00 €
BSA-FAF-1S	Bovine Serum Albumin, Fatty Acid Free, Origin USA	100 g	178.00 €
BSA-PF-1U	Bovine Serum Albumin, Protease Free, Origin USA	50 g	88.00 €
BSA-PF-1S	Bovine Serum Albumin, Protease Free, Origin USA	100 g	151.00 €
HSA-1R	Human Serum Albumin	10 g	56.00 €
HSA-1U	Human Serum Albumin	50 g	227.00 €
HSA-1S	Human Serum Albumin	100 g	420.00 €
N2-K	N2 Supplement (100x), Serum-free	5 ml	45.00 €
C21-H	NCS21 Supplement (50x), Serum-free	10 ml	45.00 €
ITS-H	ITS (100x), Insulin, Transferrin, Selenium	10 ml	25.00 €
HCS-F	Hybridoma Supplément, Serum-free	50 ml	99.00 €
GLN-B	L-Glutamine, 200 mM	100 ml	8.00 €
STA-B	Stable Glutamine, 200 mM	100 ml	14.50 €
NPY-B	Sodium Pyruvate, 100 mM	100 ml	10.50 €
NEAA-B	MEM Nonessential Amino Acids (100x)	100 ml	8.50 €
ACC-1B	Accutase Cell Detachment Solution	100 ml	26.00 €
ACM-1G	AccuMax Cell Detachment Solution	20 ml	29.00 €
ACM-1F	AccuMax Cell Detachment Solution	50 ml	69.00 €
TRY-1B	Trypsin-EDTA (0.05 %) in DPBS (1x)	100 ml	7.00 €
TRY-1B10	Trypsin-EDTA (0.5 %) in DPBS (10x)	100 ml	11.50 €
TRY-2B	Trypsin (0.25 %) in DPBS (1x)	100 ml	7.00 €
TRY-2B10	Trypsin (2.5 %) in DPBS (10x)	100 ml	11.50 €
TRY-3B	Trypsin-EDTA (0.25 %) in HBSS (1x) with Phenol Red	100 ml	9.50 €
TRY-4B	Trypsin-EDTA (0.05 %) in HBSS (1x) with Phenol Red	100 ml	8.50 €
AMP-B	Amphotericin B Solution (250 µg/ml)	100 ml	14.00 €
AAS-B	Antibiotic/Antimycotic Solution (100x)	100 ml	14.00 €
GEN-10B	Gentamicin Solution (10 mg/ml)	100 ml	50.00 €
PS-B	Penicillin/Streptomycin (100x)	100 ml	8.00 €
PSG-B	Penicillin/Streptomycin (100x), with L-Glutamine	100 ml	15.00 €
MYX-B	MycoXpert, Mycoplasma Removal Reagent (50x)	100 ml	99.00 €
LSM-B	Lymphocytes Separation Media, Density 1.077 g/ml	100 ml	12.00 €
LSM-A	Lymphocytes Separation Media, Density 1.077 g/ml	500 ml	36.50 €
FM1-F	FreezeMe One, Cryopreservation Medium with Fetal Bovine Serum	50 ml	65.00 €
FM2-F	FreezeMe Two, Cryopreservation Medium Serum-free	50 ml	70.00 €
MYX-B	MycoXpert, Mycoplasma Removal Reagent (50x)	100 ml	99.00 €
PBS-10XA	Dulbecco's PBS (10x), w/o Ca & Mg, w/o Phenol Red	500 ml	13.00 €
PBS-1A	Dulbecco's PBS (1x), w/o Ca & Mg, w/o Phenol Red	500 ml	6.50 €
PBS-2A	Dulbecco's PBS (1x), with Ca & Mg, w/o Phenol Red	500 ml	8.00 €
HBSS-1A	Hanks' Balanced Salts (1x), with Ca & Mg, w/o Phenol Red	500 ml	7.50 €
HBSS-2A	Hanks' Balanced Salts (1x), w/o Ca & Mg, w/o Phenol Red	500 ml	7.00 €
HBSS-3A	Hanks' Balanced Salts (1x), w/o Ca & Mg, with Phenol Red	500 ml	7.50 €
HEP-B	HEPES Buffer Solution (1 M)	100 ml	39.00 €
PBS-1A-P10	Dulbecco's PBS (1x) powder, w/o Ca & Mg, w/o Phenol Red	10 L	10.90 €
PBS-1A-P50	Dulbecco's PBS (1x) powder, w/o Ca & Mg, w/o Phenol Red	50 L	43.10 €

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6：我們還是Santa,Advanced Biotechnologies Inc,Athens Research & Technology,bangs,BBInternational,crystalchem,dianova,FD Neurotechnologies,Inc. FormuMax Scientific,Inc, Genebridege, Glycotope Biotechnology GmbH; iduron,Innovative Research of America, Ludger, neuroprobe,omicronbio, Polysciences,prospecbi, QA-BIO,quickzyme,RESEARCH DIETS,INC,sterlitech,；sysy,TriLink BioTechnologies,Inc,；worthington-biochem,zyagen等幾十家國外公司授權(quán)代理。

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Capricorn FM1-F說明書

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