Bionexus BN2055說(shuō)明書(shū)

時(shí)間：2018-10-11閱讀：1676

Bionexus BN2055說(shuō)明書(shū)

DNA標(biāo)記和DNA梯形圖
Bionexus提供各種即用型，室內(nèi)穩(wěn)定的DNA標(biāo)記和DNA梯形圖,。Bionexus雙鏈DNA分子量標(biāo)記用于常規(guī)瓊脂糖凝膠電泳。DNA大小范圍從50 bp到10 kb,，用于瓊脂糖凝膠電泳,。
Bionexus DNA標(biāo)記和梯子包括DNA質(zhì)量標(biāo)記（定量質(zhì)粒DNA Ladder），Hi-Lo DNA Ladders和Lo DNA Marker,。Mass DNA Ladder適用于100bp-4000bp范圍內(nèi)的大小測(cè)定和質(zhì)量定量,。Hi-Lo DNA Ladder的范圍從50bp到10kbp，Lo DNA Marker的范圍是50bp到2kb,。
Bionexus Hi-Lo DNA Ladder和Lo DNA Marker是用EcoRI切割的質(zhì)粒DNA,。

Bionexus DNA標(biāo)記物和梯子是預(yù)先混合的（包括上樣染料），含有染料的即用型分子量標(biāo)記物,，用作視覺(jué)輔助,，監(jiān)測(cè)瓊脂糖凝膠電泳過(guò)程中的遷移進(jìn)程。我們還可以提供沒(méi)有上樣染料的DNA標(biāo)記,。

Room Temprature Satble for over 2 years.

Bionexus BN2055說(shuō)明書(shū)

DNA Mass Marker DNA質(zhì)量標(biāo)記

貨號(hào)：BN2055

DNA質(zhì)量標(biāo)記

定量質(zhì)粒DNA LADDER
100bp - 4000 bp

即用型質(zhì)量梯

· 范圍：標(biāo)記從100 bp - 4000 bp,，具有均勻間隔的條帶,。易于記憶/識(shí)別帶尺寸

·易于 存儲(chǔ)和運(yùn)輸：在室溫下，穩(wěn)定2年以上

·質(zhì)量 梯級(jí)

· 的標(biāo)準(zhǔn)標(biāo)準(zhǔn)

4000 bp - 75ng
2000 bp - 200ng
1000 bp - 100ng
500 bp - 50 ng
100 bp - 20ng

內(nèi)容
1毫升預(yù)混DNA標(biāo)記與上樣染料,。

說(shuō)明
A.使用前將管倒置2-3次*混勻,。
B.每個(gè)車道加載10英尺。在200％恒定電壓下在1.5％瓊脂糖凝膠中電泳,。

注釋：
基于每次施用1μg標(biāo)記物,，體積為10μl（包括染料）。每個(gè)條帶的DNA濃度如所示,。將凝膠在含有溴化乙錠（EtBr）的TAE緩沖液中通過(guò)14cm長(zhǎng)的凝膠,，并在365nm處可視化。裝載染料由溴酚藍(lán)組成,。僅供研究使用,。不適用于動(dòng)物或人類診斷或治療用途。
上面列出的DNA帶量是通過(guò)分光光度計(jì)測(cè)定的,，但批次差異很明顯,。
存在殘留的銨離子并且可以抑制激酶交換反應(yīng)。

標(biāo)記物和染料均含有0.02％ sodium azide,。

每條帶的DNA量（ng）

	10 ul	5 ul	2 ul
4 kb	75	37.5	15
2KB	200	100	40
1 kb	100	50	20
500 bp	50	25	10
100 bp	20	10	4

100個(gè)應(yīng)用（加載染料）

也可以不加載染料,。

Bionexus部分產(chǎn)品列表

Code	Name
BN15LF	Bench Top Incubator Shaker(BN15LF) Replaced by BR11/12/13
BN2050	ALL PURPOSE HI-LO DNA MARKER / MASS LADDER
BNBR15	Bench Top Incubator Shaker(BNBR15) (Replaced by BR11/12/13)
BNE36	MICRO SHAKER(BNE36)
BNECB	Thermoelectric cooling BUCKET, ECB(+3C - ~+20C adjustable)
BNEHB	Thermoelectric Heating BUCKET, EHB(+35C - ~+70C adjustable Temp)
BNEIB	Thermoelectric Ice BUCKET, EIB(+3C fixed)
BNEM-0836	Aluminum Rack Adapter BNE36 (BNEM-0836)
BNEM-1036	Aluminum Rack Adapter for BNE36 (BNEM-1036)
BNEM-1040	Polycarbonated Rack Adapter for BNE36 (BNEM-1040)

BNMBR-024	MICRO BIO SHAKER(BNMBR-024)
BNPR1112	RACK ADAPTER FOR BNSI36(Holds 8/1.5mL Microtube)
BNPR1180	RACK ADAPTER FOR BNSI36
BNPR1808	RACK ADAPTER FOR BNSI36(Holds 8/15mL Centrifuge Tube)
BNPR3504	RACK ADAPTER FOR BNSI36(Holds 4/50mL Centrifuge Tube)
BNPR36	MILD ROCKER(BNPR36)
BNRT5	LO SPEED ROTATOR EXTRACTOR(BNRT5)
BNRT50	HI SPEED ROTATOR EXTRACTOR(BNRT50)
BNSE-0108	Adapters for BNRT50(Holds 24/1.5mL Microtubes for BNRT5&50)
BNSE-1315	Adapters for BNRT50(Holds 24/13-15mm Tubes )

BNSE-1800	Adapters for BNRT50(Holds 16/15mm CentrifudgeTubes for BNRT5&50)
BNSE-3500	Adapters for BNRT50(Holds 8/50 mL CentrifugeTubes)
BNSI36	Mild Shaker, BNSI36(Seesaw shaking mode)
BNVR36D	VOLTEX SHAKER(BNVR36D)
BNPM10	NexusView 10kDa Dual Color (250ul)
P005	Linear Acrylamide, 5 X 1 mL - 5 mg/ml
Miracle Beads	Miracle Beads
BNBAL-8188	Dual Side Aluminum block, BAL-8188(for e--> Bucket series)
BNBAL-8148	Dual Side Aluminum block,BAL-8148 (for e--> Bucket series)
BNETB	e-->Bucket, ETB (+3 to +70 C)

BNR2007	BamH I (Size=10000Units, Concentration=10U/ul)
BN2R2012	BgI I (Size=1000Units, Concentration=40U/ul)
BNR2012	BgI I (Size=1000 Units, Concentration=10U/ul)
BNR2015	BgI II (Size=500 Units, Concentration=10U/ul)
BNR2016	BgI II (Size=500 Units, Concentration=40U/ul)
BNR2017	BgI II (Size=2000 Units, Concentration=10U/ul)
BNR2018	BgI II (Size=2000 Units, Concentration=40U/ul)
BNR2021	BstE II (Size=2000 Units, Concentration=10U/ul
BNR2022	BstE ll (Size=2000 Units, Concentration=40U/ul)
BNR2023	BstE II (Size=10000 Units, Concentration=10U/ul)

BNR2007	BamH I (Size=10000Units, Concentration=10U/ul)
BN2R2012	BgI I (Size=1000Units, Concentration=40U/ul)
BNR2012	BgI I (Size=1000 Units, Concentration=10U/ul)
BNR2015	BgI II (Size=500 Units, Concentration=10U/ul)
BNR2016	BgI II (Size=500 Units, Concentration=40U/ul)
BNR2017	BgI II (Size=2000 Units, Concentration=10U/ul)
BNR2018	BgI II (Size=2000 Units, Concentration=40U/ul)
BNR2021	BstE II (Size=2000 Units, Concentration=10U/ul
BNR2022	BstE ll (Size=2000 Units, Concentration=40U/ul)
BNR2023	BstE II (Size=10000 Units, Concentration=10U/ul)

BNR2024	BstE II (Size=10000 Units, Concentration=40U/ul)
BNR2025	BstX I (Size=1000 Units, Concentration=10U/ul)
BNR2026	BstX I (Size=1000 Units, Concentration=40U/ul)
BNR2027	BstX I (Size=5000 Units, Concentration=10U/ul)
BNR2029	Cla I (Size=500 Units, Concentration=10U/ul)
BNR2030	Cla I (Size=500 Units, Concentration=40U/ul)
BNR2031	Cla I (Size=2500 Units, Concentration=10U/ul)
BNR2032	Cla I (Size=2500 Units, Concentration=40U/ul)
BNR2033	EcoR I (Size=5000Units, Concentration=10U/ul)
BNR2034	EcoR I (Size=5000Units, Concentration=40U/ul)

BN30085	X-Gal (5-Bromo-4-Chloro-3Indolyl-B-D-Galactopyranside)(5g)
BN3007	IPTG (Isopropyl-B-D-Thiogalactopyranoside)(1g)
BN30075	IPTG(Isopropyl-B-D-Thiogalactopyranoside)(5g)
BN3000	Ampicillin USP Grade Sodium Salt(5g)
BN30001	Ampicillin USP Grade Sodium Salt(10g)
BN30100	Ampicillin USP Grade Sodium Salt(100g)
BN3030	Kanamycin Sulfate USP Grade(1g)
BN3031	Kanamycin Sulfate USP Grade(5g)
BN3020	Tetracycline-HCl USP Grade(25g)
BN3022	Tetracycline-HCl USP Grade(100g)
Custom Oligo's by email	Custom Oligo's by email
Custom DNA Sequencing	Custom DNA Sequencing(Inquire for pricing)
BNMBR-022UP	Micro Bio Shaker (BNMBR-022UP)
BNAP1	(BNAP1)Standard Polyclonal Ab* (Rabbits Polyclonal Antibody service)
BNABP4	(BNABP4)Polyclonal Ab.-peptide.(Rabbits Polyclonal Antibody service)
BNABP2	(BNABP2)Polyclonal Ab.-peptide.(Rabbits Polyclonal Antibody service)
BNABP12	(BNABP12)Standard Polyclonal Ab*(Chickens Polyclonal Antibody service)
BNABP42	(BNABP42)Polyclonal Ab.-peptide.(Chickens Polyclonal Antibody service)
BNABP22	(BNABP22)Polyclonal Ab.-peptide.(Chickens Polyclonal Antibody service)
BNABP10	(BNABP10)Standard Polyclonal Ab*(Goats/Sheep's Polyclonal Antibody service)

BNABP40	(BNABP40)Polyclonal Ab.-peptide.(Goats/Sheep's Polyclonal Antibody service)
BNABP20	(BNABP20)Polyclonal Ab-peptide.(Goats/Sheep's Polyclonal Antibody service)
BNABP14	(BNABP14)Standard Polyclonal Ab*(Guinea pig Polyclonal Antibody service)
BNABP44	(BNABP44)Polyclonal Ab.-peptide.(Guinea pig Polyclonal Antibody service)
BNABP24	(BNABP24)Polyclonal Ab-peptide.(Guinea pig Polyclonal Antibody service)
BNABP11	(BNABP11)Standard Polyclonal Ab* (Mice, Polyclonal Antibody service)
BNABP41	(BNABP41)Polyclonal Ab.-peptide.(Mice, Polyclonal Antibody service)
BNABP21	(BNABP21)Polyclonal Ab-peptide.(Mice, Polyclonal Antibody service)
BNABP13	(BNABP13)Standard Polyclonal Ab*(Rats, Polyclonal Antibody service)
BNABP43	(BNABP43)Polyclonal Ab.-peptide.(Rats, Polyclonal Antibody service)

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