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上海起發(fā)實(shí)驗(yàn)試劑有限公司

PRP-X100洗脫液濃縮液

時(shí)間：2018-7-6閱讀：1963

Hamilton Company專注于精密測(cè)量設(shè)備,，自動(dòng)化液體處理工作站和樣品管理系統(tǒng)的開(kāi)發(fā),，制造和定制,。Hamilton的流程針對(duì)質(zhì)量和靈活性進(jìn)行了優(yōu)化,。無(wú)論是具有快速交付時(shí)間框架的定制針頭,，特殊長(zhǎng)度的pH傳感器，還是全面自動(dòng)化您的檢測(cè)工作流程的全面解決方案,，相信Hamilton產(chǎn)品將始終滿足您的需求,。

Hamilton公司60多年來(lái)一直是的制造商，總部位于內(nèi)華達(dá)州里諾市; 馬薩諸塞州富蘭克林; 蒂米什瓦拉,，羅馬尼亞; 和瑞士的Bonaduz; 和遍布的子公司,。

 

PRP-X100洗脫液濃縮液

PRP-X100 Eluent Concentrate

 

Hamilton為PRP-X100陰離子交換HPLC色譜柱提供了一種快速簡(jiǎn)便的方法來(lái)制備4.0 mM對(duì)羥基苯甲酸/ 2.5％甲醇，pH 8.5流動(dòng)相,。

  • 為PRP-X100陰離子交換柱快速制備流動(dòng)相
  • 沒(méi)有計(jì)算,，只需混合并運(yùn)行樣品 

零件號(hào)/ REF 

描述 

79325

Eluent Concentrate，PRP-X100陰離子交換,，（60 mL瓶裝,，每瓶1瓶）

79335

Eluent Concentrate，PRP-X100陰離子交換,，（60 mL瓶裝,，每瓶6瓶）

 

 

 

HPLC樹(shù)脂規(guī)格

Reversed Phase Columns

Packing Name

Support Material

Exchange Capacity

Pore Size

Temp. Limits

Phase Limits

Buffer Strength

Maximum Pressure

PRP-1

PSDVB*

N/A

100 Å

5-85 °C

pH 1-13. 0-100% aqueous, organic modifier

0.0-0.5N

5,000 psi

PRP-C18

C18 bonded to PSDVB*

N/A

100 Å

5-85 °C

pH 1 – 13. 0-100% aqueous, organic modifier

0.0-0.5N

5,000 psi

PRP-3

PSDVB*

N/A

300 Å

5-85 °C

pH 1-13. 0-100% aqueous, organic modifier

0.0-0.5N

5,000 psi

PRP-h5

Fluorinated PSDVB*

N/A

300 Å

5-85 °C

pH 1 – 13. 0-100% aqueous, organic modifier

0.0-0.5N

5,000 psi

HxSil C18

Silica

N/A

110 Å

5-60 °C

pH 1.5 – 10. 0-100% aqueous, organic modifier

N/A

6,000 psi

HxSil C8

Silica

N/A

110 Å

5-60 °C

pH 1.5 – 10. 0-100% aqueous, organic modifier

N/A

6,000 psi

Anion Exchange Columns

Packing Name

Support Material

Exchange Capacity

Pore Size

Temp. Limits

Phase Limits

Buffer Strength

Maximum Pressure

PRP-X100

PSDVB* with Tri- methyl ammonium Exchanger

0.19meq/gm

100 Å

pH Dependent**

pH 1-13. 0-100% aqueous, organic modifier

0.0-0.5N

5,000 psi

PRP-X110

PSDVB* with Tri-methyl ammonium Exchanger

0.11meq/gm

100 Å

pH Dependent**

pH 1-13. 0-100% aqueous, organic modifier

0.0-0.5N

5,000 psi

PRP-X500

Poly(meth-acryl amido-propyl Trimethyl-ammonium chloride)

1.6meq/gm

Superficially porous

pH Dependent**

pH 1-13. 0-100% aqueous, organic modifier

0.0-2.0N

5,000 psi

PRP-X600

Poly (di-methyl amido-propyl meth-acrylamide)

1.6meq/gm

Superficially porous

pH Dependent**

pH 1-13. 0-100% aqueous, organic modifier

0.0-1.0N

5,000 psi

RCX-10

PSDVB* with Tri-methyl ammonium Exchanger

0.35meq/gm

100 Å

pH Dependent**

pH 1-13. 0-100% aqueous, organic modifier

0.0-1.0N

5,000 psi

RCX-30

PSDVB* with Tri-methyl ammonium Exchanger

1.0meq/gm

100 Å

pH Dependent**

pH 1-13. 0-100% aqueous, organic modifier

0.0-1.0N

5,000 psi

Cation Exchange Columns

Packing Name

Support Material

Exchange Capacity

Pore Size

Temp. Limits

Phase Limits

Buffer Strength

Maximum Pressure

PRP-X200

PSDVB* Sulfonate Exchanger

35ueq/gm

100 Å

5-60 °C

pH 1-13. 0-100% aqueous, organic modifier

0.0-0.5N

5,000 psi

PRP-X400

PSDVB* Sulfonate Exchanger

2.5meq/gm

N/A

5-60 °C

pH 1-13. 0-100% aqueous, organic modifier

0.0-5.0N

5,000 psi

PRP-X800

PSDVB* Itaconate Exchanger

1.6meq/gm

100 Å

5-60 °C

pH 1-13. 0-100% aqueous, organic modifier

0.0-0.5N

5,000 psi

HC-40 Calcium

PSDVB* Sulfonate Exchanger

5meq/gm

Gel-type

25-90 °C

100% Water

Water

1,000 psi

HC-75 Calcium

PSDVB* Sulfonate Exchanger

5meq/gm

Gel-type

25-90 °C

100% Water 0-40% Acetonitrile

Water

400 psi

HC-75 Hydrogen

PSDVB* Sulfonate Exchanger

5meq/gm

Gel-type

25-90 °C

100% Water 0-40% Acetonitrile

Water

400 psi

HC-75 Lead

PSDVB* Sulfonate Exchanger

5meq/gm

Gel-type

25-90 °C

100% Water 0-40% Acetonitrile

Water

400 psi

Ion Exclusion Columns

Packing Name

Support Material

Exchange Capacity

Pore Size

Temp. Limits

Phase Limits

Buffer Strength

Maximum Pressure

PRP-X300

PSDVB* Sulfonate Exchanger

0.17meq/gm

100 Å

5-60 °C

pH 1-13. 0-100% aqueous, organic modifier

0.0-0.5N

5,000 psi

*PSDVB is Poly(styrene-divinylbenzene)
**pH 1-7.9 Temp. 5-60 °C; pH 8-13 Temp. 5-30 °C

 

 

 

HPLC色譜柱保養(yǎng)和儲(chǔ)存

 

HPLC柱具有有限的壽命。建議您使用標(biāo)準(zhǔn)定期監(jiān)測(cè)色譜柱的保留特性和性能,。如果您打算長(zhǎng)時(shí)間儲(chǔ)存??色譜柱,，請(qǐng)使用抑制微生物生長(zhǎng)的流動(dòng)相（含有sodium azide或高濃度甲醇，乙腈等的流動(dòng)相）,。為獲得適性能和色譜柱壽命,，請(qǐng)遵循以下建議。

  1. 定期監(jiān)控色譜柱的性能,。
  2. 僅在可混溶的流動(dòng)相之間切換,。
  3. 避免在色譜柱中沉淀鹽。
  4. 使用過(guò)濾和脫氣的流動(dòng)相。
  5. 不要讓色譜柱變干,。不使用時(shí)請(qǐng)將其蓋緊,。
  6. 對(duì)于長(zhǎng)期儲(chǔ)存，使用抑制細(xì)菌和霉菌生長(zhǎng)的流動(dòng)相,。
  7. 按標(biāo)簽上指示的方向使用該列,。異常高的操作壓力可能表明插入的入口玻璃料堵塞，并且可以通過(guò)使流過(guò)柱的流量反轉(zhuǎn)10-20mL來(lái)清除,。
  8. 使用Hamilton保護(hù)柱去除可能終身性結(jié)合分析柱的顆粒物質(zhì)或雜質(zhì),。

恢復(fù)色譜柱性能
下面列出的步驟可能會(huì)恢復(fù)已經(jīng)污染的色譜柱的性能，但這些過(guò)程不會(huì)改善損壞或超出其使用壽命的色譜柱的性能,。也不能恢復(fù)所有固定相,。PRP-X500和PRP-X600色譜柱的*支持使得無(wú)法通過(guò)洗滌程序恢復(fù)性能。有關(guān)這些色譜柱的技術(shù)幫助,，請(qǐng)HPLC支持,。注意：在執(zhí)行恢復(fù)程序之前，請(qǐng)務(wù)必確保流動(dòng)相是可混溶的,，并且不會(huì)發(fā)生沉淀,。如有必要，在轉(zhuǎn)換到新的流動(dòng)相之前,，先用合適的中間溶劑清洗色譜柱,。應(yīng)使用至少五個(gè)柱體積的中間溶劑。

色譜柱修復(fù)程序
按照下面列出的適當(dāng)修復(fù)程序,，使用1.0或2.0 mL / min的流速,。除非另有規(guī)定。使用每列隨附的性能測(cè)試報(bào)告中列出的測(cè)試混合物和條件評(píng)估色譜柱性能,。

柱修復(fù)程序

Packing Material Name

 

Restoration Procedure

PRP-C18

Flush with 40:40:20 (ACN:IPA:H20) for 5-10 column volumes. Equilibrate with starting phase for 5-10 column volumes.

PRP-1

Flush with 40:40:20 (ACN:IPA:H20) for 5-10 column volumes. Equilibrate with starting phase for 5-10 column volumes.

PRP-3

Flush with 40:40:20 (ACN:IPA:H20) for 5-10 column volumes. Equilibrate with starting phase for 5-10 column volumes.

PRP-h5

Flush with 40:40:20 (ACN:IPA:H20) for 5-10 column volumes. Equilibrate with starting phase for 5-10 column volumes.

HxSil C8

Flush with 40:40:20 (ACN:IPA:H20) for 5-10 column volumes. Equilibrate with starting phase for 5-10 column volumes.

HxSil C18

Flush with 40:40:20 (ACN:IPA:H20) for 5-10 column volumes. Equilibrate with starting phase for 5-10 column volumes.

PRP-X100

Pump approximay 50 mL of methanol with 1% 6 N nitric acid. Equilibrate with starting phase for ~ 200 column volumes.

PRP-X200

Inject several times with 100 µL of 1 N nitric acid. Equilibrate with starting phase for ~ 200 column volumes.

PRP-X300

Inject several times with 100 µL of 1 N nitric acid. Equilibrate with starting phase for ~ 200 column volumes.

PRP-X400

Inject Several times with 100 µL of 0.1 M potassium EDTA. Equilibrate with starting phase for ~ 200 column volumes.

RCX-10

Flush with 50 mL 0.1 N sodium hydroxide. Equilibrate with starting phase for ~ 200 column volumes.

RCX-30

Flush with 150 mL 0.1 N sodium hydroxide. Equilibrate with starting phase for ~ 200 column volumes.

HC-40

Ca2+  Flush with 1% calcium chloride at 0.1 mL/min overnight. Equilibrate with starting phase for ~ 200 column volumes.

HC-75

Ca2+  Flush with 1% calcium chloride at 0.1 mL/min overnight. Equilibrate with starting phase for ~ 200 column volumes.

 

H+      Flush with 0.1 N sulfuric acid at 0.1 mL/min overnight. Equilibrate with starting phase for ~ 200 column volumes.

 

Pb2+  Flush with 1% lead nitrate at 0.1 mL/min overnight. Equilibrate with starting phase for ~ 200 column volumes.

 

 

 

 

PRP-X100色譜柱

 

零件號(hào)/ REF 

描述 

79641

分析型HPLC色譜柱,，CUSTOM ORDER（1.0,2.1,4.1,4.6 mm ID）

79669

負(fù)離子快

79670

負(fù)離子微

79668

負(fù)離子分辨率

79643

制備型HPLC色譜柱＃1，定制（21.2,30 mm內(nèi)徑）

79644

制備型HPLC色譜柱＃2,，定制（50 mm內(nèi)徑）

79645

制備型HPLC色譜柱＃3,，CUSTOM ORDER（100 mm ID）

79421

PRP-X10010μm2.1x 150 mm

79346

PRP-X10010μm2.1x 250 mm

79439

PRP-X10010μm4.1x 100 mm

79434

PRP-X10010μm4.1x 150 mm

79433

PRP-X10010μm4.1x 250 mm

79365

PRP-X10010μm4.1x 50 mm

79354

PRP-X10010μm4.6x 150 mm PEEK

79455

PRP-X10010μm4.6x 250 mm PEEK

79353

PRP-X10012-20μm100Å21.2x 250 mm

79852

PRP-X1005μm2.1x 150 mm PEEK

79190

PRP-X1005μm2.1x 250 mm

79538

PRP-X1005μm4.1x 100 mm

79812

PRP-X1005μm4.1x 150 mm

79810

PRP-X1005μm4.1x 50 mm

79174

PRP-X1005μm4.6x 150 mm PEEK

79181

PRP-X1005μm4.6x 250 mm PEEK

79665

PRP-X1007μm4.6x 150 mm PEEK

79642

半制備型HPLC色譜柱，CUSTOM ORDER（7.0,7.8,10 mm ID）

 

 

貨號(hào)

品名

79477

Analytical Guard Cartridge Holder, PEEK

32908

Analytical Guard Cartridge Holder, Stainless Steel

79293

Analytical Guard Column, PRP-X110

79641

Analytical HPLC Column, CUSTOM ORDER (1.0, 2.1, 4.1, 4.6 mm ID)

79643

Preparative HPLC Column #1, CUSTOM ORDER (21.2, 30 mm ID)

79644

Preparative HPLC Column #2, CUSTOM ORDER (50 mm ID)

79645

Preparative HPLC Column #3, CUSTOM ORDER (100 mm ID)

79732

PRP-X110 7 µm 4.1 x 150 mm

79734

PRP-X110 7 µm 4.1 x 250 mm

79738

PRP-X110 7 µm 4.6 x 150 mm PEEK

79729

PRP-X110 Analytical Guard Column Replacement Cartridges (5/pk), PEEK

79728

PRP-X110 Analytical Guard Column Replacement Cartridges (5/pk), Stainless Steel

79727

PRP-X110 Analytical Guard Column Starter Kit (1 holder, 2 cartridges), PEEK

79726

PRP-X110 Analytical Guard Column Starter Kit (1 holder, 2 cartridges), Stainless Steel

79827

PRP-X110, 7 µm Bulk Resin

79743

PRP-X110S 7 µm 2.1 x 100 mm PEEK

79733

PRP-X110S 7 µm 4.1 x 150 mm

79735

PRP-X110S 7 µm 4.1 x 250 mm

79741

PRP-X110S 7 µm 4.6 x 250 mm PEEK

79642

Semipreparative HPLC Column, CUSTOM ORDER (7.0, 7.8, 10 mm ID)

 

 

 

 

 

上海起發(fā)實(shí)驗(yàn)試劑有限公司是實(shí)驗(yàn)試劑一站式采購(gòu)服務(wù)商

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