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上海起發(fā)實(shí)驗(yàn)試劑有限公司

Dual-Labeled BHQ Probes 說明書

時(shí)間：2017-10-30閱讀：1498

世界*實(shí)驗(yàn)材料供應(yīng)商Biosearch Technologies正式授權(quán)上海起發(fā)為其中國(guó)代理,，Biosearch Technologies在一直是行業(yè)的*,一直為廣大科研客戶提供zui為的產(chǎn)品和服務(wù),上海起發(fā)一直秉承為中國(guó)科研客戶帶來的產(chǎn)品,，的服務(wù),， 簽約Biosearch Technologies就是為了給廣大科研客戶帶來更加完善的產(chǎn)品和服務(wù),您的滿意將是我們zui大的收獲

Biosearch Technologies中國(guó)代理,，Biosearch Technologies上海代理,，Biosearch Technologies北京代理,，Biosearch Technologies廣東代理,，Biosearch Technologies江蘇代理 Biosearch Technologies湖北代理, Biosearch Technologies天津, Biosearch Technologies黑龍江代理, Biosearch Technologies內(nèi)蒙古代理, Biosearch Technologies吉林代理, Biosearch Technologies福建代理, Biosearch Technologies江蘇代理, Biosearch Technologies浙江代理, Biosearch Technologies四川代理,

 

Biosearch Technologies是一家致力于創(chuàng)新性研發(fā)新型核酸產(chǎn)品以促進(jìn)新基因的發(fā)現(xiàn)與應(yīng)用的高科技公司。從上世紀(jì)八十年代開始,一代一代的Biosearch創(chuàng)新者就專注于寡核苷酸化學(xué)合成技術(shù),， 在設(shè)計(jì)和生產(chǎn)熒光探針及引物中經(jīng)過多次改良形成了公司*的寡核苷酸修飾技術(shù),。目前 Biosearch開發(fā)的熒光粹滅技術(shù)Black Hole Quencher® (BHQ® dyes) 已成為熒光探針設(shè)計(jì)/合成的工業(yè)生產(chǎn)標(biāo)準(zhǔn)。以創(chuàng)新研發(fā)為原發(fā)動(dòng)力的Biosearch Technologies公司已經(jīng)通過英國(guó)ISO 9001:2008,、ISO 13485:2003標(biāo)準(zhǔn)認(rèn)證,，獲得加利福尼亞設(shè)備制造許可證以及GMP生產(chǎn)許可證。Biosearch擁有豐富的高品質(zhì)產(chǎn)品線,，不僅包括探針和引物產(chǎn)品,，廣泛應(yīng)用于藥物研發(fā)，DNA測(cè)序/SNP檢測(cè)/基因表達(dá)分析等領(lǐng)域,，也為其他寡核苷酸生產(chǎn)廠商提供原材料,。公司在儀器生產(chǎn)領(lǐng)域的努力也使得我們能對(duì)公司產(chǎn)品的合成和純化進(jìn)一步深入和發(fā)展。

 

Dual-Labeled BHQ® Probes

雙標(biāo)簽BHQ®探頭

 

產(chǎn)品信息

img07.jpg

雙標(biāo)簽BHQ探針是傳統(tǒng)的線性FRET探針,，其包含熒光團(tuán)和猝滅劑,，其共價(jià)連接到通常具有20至30個(gè)堿基的寡核苷酸的5'和3'末端。熒光通過Taq聚合酶的5'核酸外切酶活性釋放,，Taq聚合酶在探針與其互補(bǔ)序列雜交時(shí)切斷熒光染料,。雙標(biāo)簽BHQ探針是檢測(cè)特定目標(biāo)序列量的存在和量化的理想選擇。

我們還提供ValuMix用于基因表達(dá)和qPCR,，其在單個(gè)管中提供一個(gè)BHQ探針和兩個(gè)引物,?？梢試@遞送的0.5nmol，5nmol或20nmol的探針量靈活地調(diào)節(jié)Valumix引物量,。根據(jù)您的偏好,，探針到底漆比可以在1：1至1：4.5之間。ValuMix是一種經(jīng)濟(jì)的選擇,，提供0.5nmol的FAM-BHQ探針,，僅用$ 79混合引物。有關(guān)各種選項(xiàng),，請(qǐng)參閱產(chǎn)品列表選項(xiàng)卡,。

設(shè)計(jì)關(guān)注BHQ探針

請(qǐng)注意，長(zhǎng)度超過30個(gè)堿基的序列不可能作為末端標(biāo)記的探針進(jìn)行有效的猝滅,。對(duì)于這種情況,，請(qǐng)考慮在內(nèi)部定位淬火劑。相反,，短于20個(gè)堿基的序列可能被設(shè)計(jì)為次要粘結(jié)劑（MGB™）探針,，并且不太可能在70℃的適當(dāng)溫度下結(jié)合。請(qǐng)考慮訂購(gòu)BHQplus®探針的順序,，否則請(qǐng)[email protected]與探針設(shè)計(jì)有任何疑問,。

注意：

  • 使用內(nèi)部淬火器構(gòu)建探頭（目前僅適用于FAM）
  • 添加由T（...）注釋的內(nèi)部修飾表示胸苷堿基附加到內(nèi)部修飾。請(qǐng)注意,，添加此類內(nèi)部修改時(shí),，將在序列中引入額外的T基,。
  • 建筑探頭與搖擺/退化基地（什么是擺動(dòng),？）
  • 為了確保對(duì)寡核苷酸進(jìn)行zui有效的處理，在序列輸入字段中輸入擺動(dòng)/退化堿基時(shí)使用IUPAC代碼,，而不是使用括號(hào)（例如（A / G）,，（A / G / C / T）等）

 

?部分相關(guān)產(chǎn)品如下：

貨號(hào)品名NOTE
DLO-FB1-1Dual-labeled Probe, 5' FAM/3' BHQ-1Provides > 60 nmol pure.
DLO-FB1-2Dual-labeled Probe, 5' FAM/3' BHQ-1Provides > 25 nmol pure.
DLO-FB1-5Dual-labeled Probe, 5' FAM/3' BHQ-1Provides > 10 nmol pure.
DLO-FT-1Dual-labeled Probe, 5' FAM/3' TAMRAProvides > 60 nmol pure.
DLO-FT-2Dual-labeled Probe, 5' FAM/3' TAMRAProvides > 25 nmol pure.
DLO-FT-5Dual-labeled Probe, 5' FAM/3' TAMRAProvides > 10 nmol pure.
DLO-HB1-1Dual-labeled Probe, 5' HEX/3' BHQ-1Provides > 60 nmol pure.
DLO-HB1-2Dual-labeled Probe, 5' HEX/3' BHQ-1Provides > 25 nmol pure.
DLO-HB1-5Dual-labeled Probe, 5' HEX/3' BHQ-1Provides > 10 nmol pure.
DLO-JB1-1Dual-labeled Probe, 5' JOE/3' BHQ-1Provides > 30 nmol pure.
DLO-JB1-2Dual-labeled Probe, 5' JOE/3' BHQ-1Provides > 10 nmol pure.
DLO-RB2-1Dual-labeled Probe, 5' ROX/3' BHQ-2Provides > 30 nmol pure.
DLO-RB2-2Dual-labeled Probe, 5' ROX/3' BHQ-2Provides > 10 nmol pure.
DLO-TEB1-1Dual-labeled Probe, 5' TET/3' BHQ-1Provides > 60 nmol pure.
DLO-TEB1-2Dual-labeled Probe, 5' TET/3' BHQ-1Provides > 25 nmol pure.
DLO-TEB1-5Dual-labeled Probe, 5' TET/3' BHQ-1Provides > 10 nmol pure.
DLO-TB2-1Dual-labeled Probe, 5' TAMRA/3' BHQ-2Provides > 60 nmol pure.
DLO-TB2-2Dual-labeled Probe, 5' TAMRA/3' BHQ-2Provides > 25 nmol pure.
DLO-TB2-5Dual-labeled Probe, 5' TAMRA/3' BHQ-2Provides > 10 nmol pure.
DLO-TET-1Dual-labeled Probe, 5' TET/3' TAMRAProvides > 60 nmol pure.
DLO-TET-2Dual-labeled Probe, 5' TET/3' TAMRAProvides > 25 nmol pure.
DLO-TET-5Dual-labeled Probe, 5' TET/3' TAMRAProvides > 10 nmol pure.
DLO-CAB2-2Dual-labeled Probe, 5' CAL Fluor Red 610/3' BHQ-2Provides > 25 nmol pure.
DLO-CAB2-5Dual-labeled Probe, 5' CAL Fluor Red 610/3' BHQ-2Provides > 10 nmol pure.
DLO-CAB2-1Dual-labeled Probe, 5' CAL Fluor Red 610/3' BHQ-2Provides > 60 nmol pure.
DLO-Q6B2-5Dual-labeled Probe, 5' Quasar 670/3’ BHQ-2Provides 10 nmol pure.
DLO-Q6B2-2Dual-labeled Probe, 5' Quasar 670/3’ BHQ-2Provides > 20 nmol pure.
DLO-Q6B2-1Dual-labeled Probe, 5' Quasar 670/3’ BHQ-2Provides > 40 nmol pure.
DLO-Q5B2-5Dual-labeled Probe, 5' Quasar 570/3’ BHQ-2Provides > 10 nmol pure.
DLO-Q5B2-2Dual-labeled Probe, 5' Quasar 570/3’ BHQ-2Provides > 25 nmol pure.
DLO-Q5B2-1Dual-labeled Probe, 5' Quasar 570/3’ BHQ-2Provides > 60 nmol pure.
DLO-Q6B3-2Dual-labeled Probe, 5' Quasar 670/3’ BHQ-3Provides > 20 nmol pure.
DLO-Q6B3-1Dual-labeled Probe, 5' Quasar 670/3’ BHQ-3Provides > 40 nmol pure.
DLO-COB1-1Dual-labeled Probe, 5' CAL Fluor® Orange 560/3' BHQ-1Provides > 60 nmol pure.
DLO-COB1-2Dual-labeled Probe, 5' CAL Fluor® Orange 560/3' BHQ-1Provides > 25 nmol pure.
DLO-COB1-5Dual-labeled Probe, 5' CAL Fluor® Orange 560/3' BHQ-1Provides > 10 nmol pure.
DLO-B2P6-2Dual-labeled Probe, 5’ BHQ-2/3’ Pulsar 650Provides > 10 nmol pure.
DLO-B2P6-1Dual-labeled Probe, 5’ BHQ-2/3’ Pulsar 650Provides > 40 nmol pure.
DLO-CGB1-5Dual-labeled Probe, 5' CAL Fluor Gold 540/3' BHQ-1Provides > 10 nmol pure.
DLO-CGB1-2Dual-labeled Probe, 5' CAL Fluor Gold 540/3' BHQ-1Provides > 25 nmol pure.
DLO-CGB1-1Dual-labeled Probe, 5' CAL Fluor Gold 540/3' BHQ-1Provides > 60 nmol pure.
DLO-C635B2-5Dual-labeled Probe, 5' CAL Fluor® Red 635/3' BHQ-2Provides > 3 nmol pure.
DLO-C590B2-5Dual-labeled Probe, 5' CAL Fluor Red 590/3' BHQ-2Provides > 10 nmol pure.
DLO-F(T-B1)-2Dual-labeled Probe, 5' FAM/Internal T-BHQ-1/3’ C3Provides > 10 nmol pure.
DLO-F(T-B1)-1Dual-labeled Probe, 5' FAM/Internal T-BHQ-1/3’ C3Provides > 30 nmol pure.
DLO-C590B2-2Dual-labeled Probe, 5' CAL Fluor Red 590/3' BHQ-2Provides > 25 nmol pure.
DLO-C590B2-1Dual-labeled Probe, 5' CAL Fluor Red 590/3' BHQ-2Provides > 60 nmol pure.
DLO-C635B2-2Dual-labeled Probe, 5' CAL Fluor® Red 635/3' BHQ-2Provides > 10 nmol pure.
DLO-C635B2-1Dual-labeled Probe, 5' CAL Fluor® Red 635/3' BHQ-2Provides > 40 nmol pure.
DLO-RFB-5ValuProbe™, 5' FAM/3' BHQ-1Provides 10 nmol pure.
DLO-Q7B2-5Dual-labeled Probe, 5' Quasar 705/3’ BHQ-2Provides > 3 nmol pure.
DLO-Q7B2-2Dual-labeled Probe, 5' Quasar 705/3’ BHQ-2Provides > 10 nmol pure.
DLO-Q7B2-1Dual-labeled Probe, 5' Quasar 705/3’ BHQ-2Provides > 40 nmol pure.
DLO-FT-25Dual-labeled Probe, 5' FAM/3' TAMRAProvides 5 nmol pure.
DLO-TEB1-25Dual-labeled Probe, 5' TET/3' BHQ-1Provides 5 nmol pure.
DLO-TET-25Dual-labeled Probe, 5' TET/3' TAMRAProvides 5 nmol pure.
DLO-HB1-25Dual-labeled Probe, 5' HEX/3' BHQ-1Provides 5 nmol pure.
DLO-Q6B2-25Dual-labeled Probe, 5' Quasar 670/3’ BHQ-2Provides 5 nmol pure.
DLO-Q5B2-25Dual-labeled Probe, 5' Quasar 570/3’ BHQ-2Provides 5 nmol pure.
DLO-CAB2-25Dual-labeled Probe, 5' CAL Fluor Red 610/3' BHQ-2Provides 5 nmol pure.
DLO-COB1-25Dual-labeled Probe, 5' CAL Fluor® Orange 560/3' BHQ-1Provides 5 nmol pure.
DLO-CGB1-25Dual-labeled Probe, 5' CAL Fluor Gold 540/3' BHQ-1Provides 5 nmol pure.
DLO-C590B2-25Dual-labeled Probe, 5' CAL Fluor Red 590/3' BHQ-2Provides 5 nmol pure.
DLO-TB2-25Dual-labeled Probe, 5' TAMRA/3' BHQ-2Provides 5 nmol pure
DLO-Q7B3-2Dual-labeled Probe, 5' Quasar 705/3’ BHQ-3Provides > 10 nmol pure.
DLO-Q7B3-1Dual-labeled Probe, 5' Quasar 705/3’ BHQ-3Provides > 40 nmol pure.
DLO-Q6B3-5Dual-labeled Probe, 5' Quasar 670/3’ BHQ-3Provides 10 nmol pure.
DLO-Q6B3-25Dual-labeled Probe, 5' Quasar 670/3’ BHQ-3Provides 5 nmol pure.
DLO-Q7B3-5Dual-labeled Probe, 5' Quasar 705/3’ BHQ-3Provides > 3 nmol pure.
DLOM-RFB-05FAM Dual-labeled BHQ-1 Probe with Primers, Mixed0.5 nmol
DLOM-RFB-5FAM Dual-labeled BHQ-1 Probe with Primers, Mixed5 nmol
DLOM-RFB-20FAM Dual-labeled BHQ-1 Probe with Primers, Mixed20 nmol
DLOM-COB1-5CAL Fluor Orange 560 Dual-labeled BHQ-1 Probe with Primers, Mixed5 nmol
DLOM-COB1-20CAL Fluor Orange 560 Dual-labeled BHQ-1 Probe with Primers, Mixed20 nmol
DLOM-CAB2-5CAL Fluor Red 610 Dual-labeled BHQ-2 Probe with Primers, Mixed5 nmol
DLOM-CAB2-20CAL Fluor Red 610 Dual-labeled BHQ-2 Probe with Primers, Mixed20 nmol
DLOM-Q6B2-5Quasar 670 Dual-labeled BHQ-2 Probe with Primers, Mixed5 nmol
DLOM-Q6B2-20Quasar 670 Dual-labeled BHQ-2 Probe with Primers, Mixed20 nmol
DLOM-F(T-B1)-5FAM Dual-labeled Internal T-BHQ-1 Probe with Primers, Mixed5 nmol
DLOM-F(T-B1)-20FAM Dual-labeled Internal T-BHQ-1 Probe with Primers, Mixed20 nmol

 

我們公司zui大優(yōu)勢(shì)是強(qiáng)大的采購(gòu)，

1：基本什么都能進(jìn)口,，血清,，抗體，耗材,，還有部分限制進(jìn)口的,，

2：貨品全，現(xiàn)經(jīng)營(yíng)過700多個(gè)品牌,，基本所有生物試劑耗材都可以進(jìn)口,，特別是冷偏的產(chǎn)品那就更有優(yōu)勢(shì)，

3：提供加急服務(wù),，一般1-2周到貨,，超過時(shí)限加急費(fèi)全免

4：價(jià)格公道,，絕大部分價(jià)格有優(yōu)勢(shì)，當(dāng)然不能保證100%產(chǎn)品都是,因?yàn)橐馕吨鴽]有服務(wù).

5：良好的信譽(yù),，大部分客戶我們提供貨到付款服務(wù),，客戶包括清華，北大 交大 復(fù)旦,，中山等100多所大學(xué),，ROCHE，阿斯利康,，國(guó)藥 ,，fisher等500多家公司

6：我們*代理的品牌有：Antibody Research Corporation，arcticzymes,，Biorelevant,，AmberGen, Inc. ，clemente-associates,，clodronaiposomes,，Columbia Biosciences，enzyme research,，Gene Bridges GmbH,，Genovis，AmberGen, Inc.  Biotechnology GmbH,，Haematologic Technologies HTI Haemtech,，hookelabs，Immudex,，Innovative Research of America,，inspiralis ，List Biological Laboratories, Inc.,，lumafluor,，Microsurfaces，multiplicom,，nanotools,，Pel-Freez Biologicals，pentapharm,，progen,，Protein Ark，QA-Bio,Inc,，QA-Bio,IncQuickZyme Biosciences,，Teknova，TriLink BioTechnologies, Inc.,，Zyagen Laboratories 等

7：我們還是invitrogen,qiagen,Midland BioProducts Corporationam,sigma;neb,roche,merck, rnd,BD, GE,pierce,BioLegend等知名*批發(fā),，歡迎合作,。

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